FastQCFastQC Report
Fri 10 Feb 2017
ERR1632866.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632866.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257076
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGAGACTGCAGAGGGACCAGGGGCTTTAGCTGTTGGCA5650.21977936485708507No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5440.21161057430487482No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3750.14587125986089716No Hit
CTCTACCACCATGAATCTACTTCTGATCCTTACCTTTGTTGCA3730.14509327980830572No Hit
GTTGTAGACCTTGGTGTAGACTCCAGGCCTGTTCTTCTGGGCA3620.14081438951905273No Hit
CTTTAAAGCCTTAGGCCGTATGACAAAATGAAGAGACTGAAAT3610.140425399492757No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG3550.13809145933498265No Hit
GTGTGGGGTCATGGAGCCCAATCCAGACGTATGAGTAGCTGTT3090.12019791812537928No Hit
ACCCCACACAGGGCACCGAGCCCAATGGAGAAGGTTGGGAGTG3080.11980892809908354No Hit
GTGTTAATCCTGGCTACTGTGTGAGCCTGACCTCAAGCACAGG2800.1089172073628032No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA2690.10463831707355023No Hit
GATTAAGGAGAGTGGCACTGATGACTTCAATGTCTGGATTGGC2680.1042493270472545No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTACC352.3811535E-531.7142852
CTCGGAG250.005488506529.612
CGAACCG453.9923616E-628.77777731
ACCCCGC358.8505977E-426.428576
GCGAACC509.055288E-625.90000230
AGGCGAA509.055288E-625.90000228
GGTATCA1250.025.1600021
CGAACTA451.319312E-424.66666629
CTCTAGT1002.0008883E-1124.05000127
GTATCAA3550.023.9718321
ATCCATA551.8952185E-523.54545413
GGCGAAC551.8952185E-523.54545429
ATCATTA551.8952185E-523.5454541
GCAATTA802.7050191E-823.12500217
TTACCCC400.001927132823.1250024
TGCTTAA753.7174323E-722.234
ATGCTAA502.6942018E-422.25
CACCTCT502.6942018E-422.230
ATATATA502.6942018E-422.22
CTTACCC502.6942018E-422.23
Produced by FastQC (version 0.11.5)