##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632866.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	257076
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28905070873983	33.0	33.0	33.0	27.0	33.0
2	30.918957817921548	33.0	33.0	33.0	27.0	33.0
3	31.364405856633837	33.0	33.0	33.0	27.0	33.0
4	31.511269041061787	33.0	33.0	33.0	27.0	33.0
5	31.63489396131883	33.0	33.0	33.0	27.0	33.0
6	34.38054116292458	37.0	33.0	37.0	27.0	37.0
7	34.55153339868366	37.0	37.0	37.0	27.0	37.0
8	34.719145311114225	37.0	37.0	37.0	27.0	37.0
9	34.794232055890085	37.0	37.0	37.0	27.0	37.0
10	34.866996530208965	37.0	37.0	37.0	27.0	37.0
11	34.886095940500084	37.0	37.0	37.0	27.0	37.0
12	34.849756492243536	37.0	37.0	37.0	27.0	37.0
13	34.881910407817145	37.0	37.0	37.0	27.0	37.0
14	34.83714543559103	37.0	37.0	37.0	27.0	37.0
15	34.867509997043676	37.0	37.0	37.0	27.0	37.0
16	34.87033017473432	37.0	37.0	37.0	27.0	37.0
17	34.8249467083664	37.0	37.0	37.0	27.0	37.0
18	34.83460144081906	37.0	37.0	37.0	27.0	37.0
19	34.81665733090603	37.0	37.0	37.0	27.0	37.0
20	34.834624780220636	37.0	37.0	37.0	27.0	37.0
21	34.82630039365791	37.0	37.0	37.0	27.0	37.0
22	34.83004247771087	37.0	37.0	37.0	27.0	37.0
23	34.824176508114334	37.0	37.0	37.0	27.0	37.0
24	34.82771631735362	37.0	37.0	37.0	27.0	37.0
25	34.83334889293439	37.0	37.0	37.0	27.0	37.0
26	34.77006021565607	37.0	37.0	37.0	27.0	37.0
27	34.688527906144486	37.0	37.0	37.0	27.0	37.0
28	34.673703496242354	37.0	37.0	37.0	27.0	37.0
29	34.71487031072523	37.0	37.0	37.0	27.0	37.0
30	34.67811464314055	37.0	37.0	37.0	27.0	37.0
31	34.68648570850643	37.0	37.0	37.0	27.0	37.0
32	34.721961598904606	37.0	37.0	37.0	27.0	37.0
33	34.6426893214458	37.0	37.0	37.0	27.0	37.0
34	34.61306773094338	37.0	37.0	37.0	27.0	37.0
35	34.59860119186544	37.0	37.0	37.0	27.0	37.0
36	34.57857598531174	37.0	37.0	37.0	27.0	37.0
37	34.61234809939473	37.0	37.0	37.0	27.0	37.0
38	34.4614822075962	37.0	37.0	37.0	27.0	37.0
39	34.53247677729543	37.0	37.0	37.0	27.0	37.0
40	34.577840794162036	37.0	37.0	37.0	27.0	37.0
41	34.57883271872909	37.0	37.0	37.0	27.0	37.0
42	34.47727131276354	37.0	37.0	37.0	27.0	37.0
43	34.233335667273494	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	10.0
16	11.0
17	17.0
18	15.0
19	16.0
20	38.0
21	89.0
22	274.0
23	615.0
24	1167.0
25	1982.0
26	3009.0
27	4263.0
28	6026.0
29	7555.0
30	9594.0
31	11993.0
32	15044.0
33	19279.0
34	26508.0
35	46153.0
36	103418.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.698563848822914	19.755636465481025	12.497860554855373	26.047939130840685
2	16.884890071418567	22.937574880580062	36.0298121956153	24.147722852386064
3	19.183043146773716	24.69619878946304	29.977516376480107	26.143241687283137
4	14.205137780267313	17.271546157556518	37.04352020414197	31.479795858034198
5	15.384944530022251	35.90027851685883	34.26185252610124	14.452924427017692
6	31.976925111640135	38.06539700322084	16.26211703931911	13.695560845819912
7	28.321585834539203	31.175605657470946	22.0059437676018	18.496864740388055
8	25.441114689819354	34.85895221646517	20.403693849289702	19.296239244425774
9	26.374690752929098	14.738443106318755	19.379872100079353	39.5069940406728
10	14.735331186108388	28.361262817221366	33.162177721763214	23.74122827490703
11	34.99432074561608	22.83138060340133	23.411364732608256	18.762933918374333
12	23.960229659711523	24.474085484448178	30.276260716675225	21.28942413916507
13	29.27538937901632	21.235743515536264	25.832049666246558	23.656817439200857
14	23.270939333115496	19.787144657610977	25.98920163687003	30.952714372403488
15	24.243025408828515	28.596212793103987	23.49110768799888	23.66965411006862
16	24.70981344038339	27.02313712676407	23.90732701613531	24.359722416717236
17	23.013816925734023	26.969845493161554	25.592042820022094	24.424294761082326
18	24.79539124616845	25.770589242091834	26.361076102008745	23.072943409730975
19	25.425944078793822	25.615771211626132	25.77253419222331	23.185750517356734
20	24.82417650811433	26.420591576031992	25.92190636232087	22.833325553532806
21	24.08237252796838	26.123403195942057	26.98151519395043	22.812709082139136
22	23.79490889853584	25.55236583733993	26.512393222237783	24.140332041886445
23	23.771958486984392	25.651947284071635	26.289112947144034	24.286981281799935
24	23.440927974606733	26.09889682428543	25.8281597659836	24.632015435124245
25	24.731985871882245	26.703776315175283	25.765532371749988	22.79870544119249
26	25.250509576934448	25.415441348083835	25.553532807418815	23.7805162675629
27	24.473696494421883	25.80637632451104	25.89389908042758	23.826028100639498
28	23.789463038167703	25.611881311363177	26.31789820908992	24.2807574413792
29	24.12438345080832	25.595932720285052	25.430611959109367	24.84907186979726
30	23.653705518990492	26.066999642129176	26.586690317260263	23.692604521620066
31	24.133330221413125	26.05299600118253	26.549347274735872	23.26432650266847
32	24.20101448598858	25.73869205993558	26.28872395711774	23.771569496958097
33	24.312265633509156	25.739470039988177	26.374690752929098	23.573573573573572
34	24.571721981048405	25.06768426457546	25.90751373134793	24.45308002302821
35	23.677044920568235	26.15257744791424	26.510837262132597	23.659540369384928
36	24.445689212528592	25.027618291867	26.857427375562093	23.669265120042322
37	24.744822542750004	25.480791672501518	25.950691624266753	23.823694160481725
38	24.56472016057508	25.334531422614326	26.700664394964914	23.40008402184568
39	24.415347990477525	25.72896730927819	26.38052560332353	23.475159096920756
40	24.04697443557547	26.429538346636793	26.272775366039614	23.25071185174812
41	24.9817174687641	24.968880797896343	26.151410477835345	23.89799125550421
42	24.271810670774403	25.64261152344054	25.678787595886043	24.406790209899018
43	23.986680981499635	25.537195226314395	26.366910952403185	24.10921283978279
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	7.0
2	12.0
3	31.0
4	50.0
5	50.0
6	72.0
7	94.0
8	88.5
9	83.0
10	97.5
11	112.0
12	112.0
13	197.0
14	282.0
15	506.0
16	730.0
17	843.0
18	956.0
19	956.0
20	954.5
21	953.0
22	1078.0
23	1203.0
24	1643.5
25	2084.0
26	2084.0
27	2796.0
28	3508.0
29	4460.5
30	5413.0
31	6470.5
32	7528.0
33	7528.0
34	9226.0
35	10924.0
36	12348.5
37	13773.0
38	14648.0
39	15523.0
40	15523.0
41	16413.5
42	17304.0
43	18687.5
44	20071.0
45	20806.0
46	21541.0
47	21541.0
48	21064.5
49	20588.0
50	20883.0
51	21178.0
52	20824.0
53	20470.0
54	20470.0
55	19344.0
56	18218.0
57	15979.5
58	13741.0
59	13359.5
60	12978.0
61	12978.0
62	12193.0
63	11408.0
64	8970.0
65	6532.0
66	5305.0
67	4078.0
68	4078.0
69	3248.5
70	2419.0
71	1954.5
72	1490.0
73	1190.0
74	890.0
75	890.0
76	652.5
77	415.0
78	326.0
79	237.0
80	178.5
81	120.0
82	120.0
83	99.5
84	79.0
85	63.5
86	48.0
87	37.0
88	26.0
89	26.0
90	19.5
91	13.0
92	7.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	257076.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.600442624923545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.49773714247574	53.89899336655718
2	6.225443544540324	7.669801557603138
3	1.9852202607509504	3.6687134031067403
4	1.053485835378269	2.5958077503355486
5	0.5934896224451913	1.8279611717961268
6	0.41598262532459185	1.5374828306563553
7	0.31507130226796276	1.3585972174682308
8	0.24281688048233677	1.1966101851612083
9	0.17903432301986977	0.9925734188769766
>10	1.3310170099010408	15.9574522473617
>50	0.12477995206244472	5.111910891797353
>100	0.03466109779512353	3.753574302307762
>500	0.0012604035561863103	0.4305216569716939
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATAGAGACTGCAGAGGGACCAGGGGCTTTAGCTGTTGGCA	565	0.21977936485708507	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	544	0.21161057430487482	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	375	0.14587125986089716	No Hit
CTCTACCACCATGAATCTACTTCTGATCCTTACCTTTGTTGCA	373	0.14509327980830572	No Hit
GTTGTAGACCTTGGTGTAGACTCCAGGCCTGTTCTTCTGGGCA	362	0.14081438951905273	No Hit
CTTTAAAGCCTTAGGCCGTATGACAAAATGAAGAGACTGAAAT	361	0.140425399492757	No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG	355	0.13809145933498265	No Hit
GTGTGGGGTCATGGAGCCCAATCCAGACGTATGAGTAGCTGTT	309	0.12019791812537928	No Hit
ACCCCACACAGGGCACCGAGCCCAATGGAGAAGGTTGGGAGTG	308	0.11980892809908354	No Hit
GTGTTAATCCTGGCTACTGTGTGAGCCTGACCTCAAGCACAGG	280	0.1089172073628032	No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA	269	0.10463831707355023	No Hit
GATTAAGGAGAGTGGCACTGATGACTTCAATGTCTGGATTGGC	268	0.1042493270472545	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	7.779800525914515E-4	0.0	0.0
7	0.0	0.0	0.001944950131478629	0.0	0.0
8	0.0	0.0	0.0027229301840700805	0.0	0.0
9	0.0	0.0	0.003500910236661532	3.8899002629572577E-4	0.0
10	0.0	0.0	0.004278890289252983	3.8899002629572577E-4	0.0
11	0.0	0.0	0.006612830447027338	3.8899002629572577E-4	0.0
12	0.0	0.0	0.00739081049961879	3.8899002629572577E-4	0.0
13	0.0	0.0	0.00739081049961879	3.8899002629572577E-4	0.0
14	0.0	0.0	0.008168790552210242	3.8899002629572577E-4	0.0
15	0.0	0.0	0.008946770604801693	3.8899002629572577E-4	0.0
16	0.0	0.0	0.010502730709984595	3.8899002629572577E-4	0.0
17	0.0	0.0	0.010502730709984595	3.8899002629572577E-4	0.0
18	0.0	0.0	0.010502730709984595	3.8899002629572577E-4	0.0
19	0.0	0.0	0.010891720736280322	3.8899002629572577E-4	0.0
20	0.0	0.0	0.011280710762576047	3.8899002629572577E-4	0.0
21	0.0	0.0	0.011280710762576047	7.779800525914515E-4	0.0
22	0.0	0.0	0.011280710762576047	7.779800525914515E-4	0.0
23	0.0	0.0	0.011280710762576047	7.779800525914515E-4	3.8899002629572577E-4
24	0.0	0.0	0.011669700788871773	0.0011669700788871774	3.8899002629572577E-4
25	0.0	0.0	0.011669700788871773	0.0011669700788871774	3.8899002629572577E-4
26	0.0	0.0	0.011669700788871773	0.001944950131478629	3.8899002629572577E-4
27	0.0	0.0	0.011669700788871773	0.001944950131478629	7.779800525914515E-4
28	0.0	0.0	0.011669700788871773	0.006223840420731612	7.779800525914515E-4
29	0.0	0.0	0.011669700788871773	0.016726571130716208	7.779800525914515E-4
30	0.0	0.0	0.011669700788871773	0.03539809239291104	7.779800525914515E-4
31	0.0	0.0	0.011669700788871773	0.07896497533803233	7.779800525914515E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTACC	35	2.3811535E-5	31.714285	2
CTCGGAG	25	0.0054885065	29.6	12
CGAACCG	45	3.9923616E-6	28.777777	31
ACCCCGC	35	8.8505977E-4	26.42857	6
GCGAACC	50	9.055288E-6	25.900002	30
AGGCGAA	50	9.055288E-6	25.900002	28
GGTATCA	125	0.0	25.160002	1
CGAACTA	45	1.319312E-4	24.666666	29
CTCTAGT	100	2.0008883E-11	24.050001	27
GTATCAA	355	0.0	23.971832	1
ATCCATA	55	1.8952185E-5	23.545454	13
GGCGAAC	55	1.8952185E-5	23.545454	29
ATCATTA	55	1.8952185E-5	23.545454	1
GCAATTA	80	2.7050191E-8	23.125002	17
TTACCCC	40	0.0019271328	23.125002	4
TGCTTAA	75	3.7174323E-7	22.2	34
ATGCTAA	50	2.6942018E-4	22.2	5
CACCTCT	50	2.6942018E-4	22.2	30
ATATATA	50	2.6942018E-4	22.2	2
CTTACCC	50	2.6942018E-4	22.2	3
>>END_MODULE