FastQCFastQC Report
Fri 10 Feb 2017
ERR1632865.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632865.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425641
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16590.3897650837207882No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12150.2854518244248087No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA6250.14683735824321437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6010.14119880368667492No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCC303.5957035E-430.83333427
GGTATCA3550.027.6197191
CGCCGTC358.860182E-426.42857226
TAGGACC901.4188117E-1024.6666664
ACTAGCG400.001929200323.12527
CGCAGAA400.001929200323.12529
TCTCCGA400.001929200323.12511
GTATCAA9250.021.4000021
CGATGTT705.089474E-621.14285920
TAGCGCT450.0038219120.55555529
ACCGACG450.0038219120.55555512
GCCGGCA450.0038219120.55555515
ACGGGCG450.0038219120.55555516
AGCGCTC450.0038219120.55555530
CTAGCGC450.0038219120.55555528
CGAGTCG500.00702778118.521
ATAGGAC500.00702778118.53
CGGTTCG500.00702778118.522
CGGGATA500.00702778118.54
GTCCGGC609.223792E-418.515
Produced by FastQC (version 0.11.5)