FastQCFastQC Report
Fri 10 Feb 2017
ERR1632864.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632864.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183547
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT57863.152326107209598No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT48502.642374977526192No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26571.4475856320179572No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11130.6063841958735365No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG9230.5028684751044691No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7360.40098721308438706No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG7280.39662865642042633No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7240.394449378088446No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7060.3846426255945343No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6130.3339744043759909No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5290.28820955940440324No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5100.2778579873274965No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4980.27132015233155543No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4760.2593341215056634No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4450.2424447144328156No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4160.22664494652595793No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT3810.20757626112112973No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3800.20703144153813463No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3780.20594180237214443No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3530.19232131279726716No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3490.19014203446528682No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3490.19014203446528682No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3480.18959721488229173No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3370.18360419946934572No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG3360.18305937988635063No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3340.18196974072036046No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT3250.17706636447340462No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3150.17161816864345372No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3130.17052852947746355No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT3110.16943889031147336No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3090.1683492511454832No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3060.16671479239649792No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC3000.16344587489852735No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2950.1607217769835519No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG2930.1596321378175617No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2930.1596321378175617No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2880.15690803990258625No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2850.155273581153601No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA2840.1547287615706059No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT2830.1541839419876108No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC2830.1541839419876108No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2730.1487357461576599No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC2730.1487357461576599No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2720.1481909265746648No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2710.14764610699166972No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2680.14601164824268445No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC2680.14601164824268445No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA2600.14165309157872372No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2560.13947381324674335No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT2440.13293597825080222No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2440.13293597825080222No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG2440.13293597825080222No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG2410.13130151950181695No Hit
TATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCGG2380.1296670607528317No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG2360.12857742158684152No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.12748778242085132No Hit
CTTCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTCGCCG2330.12694296283785625No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG2330.12694296283785625No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2300.12530850408887095No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT2300.12530850408887095No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2300.12530850408887095No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG2270.1236740453398857No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2250.12258440617389552No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2220.12094994742491023No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2200.11986030825892005No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2190.11931548867592497No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2180.11877066909292988No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2150.1171362103439446No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2120.11550175159495933No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC2080.11332247326297896No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT2060.11223283409698878No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC1980.10787427743302806No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA1970.10732945785003296No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG1960.10678463826703788No Hit
TATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATGA1950.1062398186840428No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA1930.10515017951805261No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG1910.10406054035206243No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC1890.10297090118607222No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1880.10242608160307713No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC1870.10188126202008205No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG1860.10133644243708695No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.10133644243708695No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT1860.10133644243708695No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTTT251.2295868E-437.01
TCCGACC200.001837908837.035
CGACCTG251.2295868E-437.037
AACTTTT250.00548446329.62
GGCCGTC358.840904E-426.4285719
GGTATCA9100.025.6153871
GAATCAA451.3173016E-424.6666661
GTATCAA24100.022.9522821
TTCGCCG2100.022.9047624
GCCGCTC2150.022.37209327
ATGGAGG502.6901296E-422.228
CGCTCTC2100.022.0238129
CCGCTCT2200.021.86363628
GCCGGCA2300.021.71739215
CAGCTTC2400.021.58333220
GCAGCTT2350.021.2553219
GGCAGCT2350.021.2553218
GATAACT450.003813751720.55555537
GGAGGGG450.003813751720.55555530
GCTCTCT2250.020.55555530
Produced by FastQC (version 0.11.5)