FastQCFastQC Report
Fri 10 Feb 2017
ERR1632863.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632863.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376211
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10270.2729851067618969No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7470.1985587874889357No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4800.1275879758965049No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4380.11642402800556072No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA3850.10233618900032163No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCCG250.005491703729.59999821
GGTATCA2700.029.4629631
GTATCAA8100.023.0679021
GTATTAG1500.020.9666671
TCGGGAT851.2407581E-619.58823422
AACGGCC1103.8311555E-818.537
TTAACGG1103.8311555E-818.535
GACTGTG609.221078E-418.57
CGGGTCA500.007026304518.49999816
GTTTCGG500.007026304518.49999821
TCGGGGG500.007026304518.49999824
CCAAACG500.007026304518.49999829
GTATAGG1451.546141E-1017.8620681
ATTAGAG1156.3710104E-817.695653
CCATCGG852.7152404E-517.41176419
GCTATAT752.062612E-417.26666619
ACGGGTC752.062612E-417.26666615
TATTAGA1107.773324E-716.8181822
TACCGCA904.4331777E-516.4444457
GGCACCG1251.6489139E-716.2799999
Produced by FastQC (version 0.11.5)