##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632863.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	376211
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.738662612204322	33.0	33.0	33.0	27.0	33.0
2	31.287290908559292	33.0	33.0	33.0	27.0	33.0
3	31.715720699288433	33.0	33.0	33.0	27.0	33.0
4	31.82554470762418	33.0	33.0	33.0	27.0	33.0
5	31.941482306471634	33.0	33.0	33.0	33.0	33.0
6	34.84677481519679	37.0	37.0	37.0	27.0	37.0
7	34.97039693150918	37.0	37.0	37.0	27.0	37.0
8	35.15951420878177	37.0	37.0	37.0	33.0	37.0
9	35.219674597499804	37.0	37.0	37.0	33.0	37.0
10	35.28270837375835	37.0	37.0	37.0	33.0	37.0
11	35.3183001028678	37.0	37.0	37.0	33.0	37.0
12	35.238876587872234	37.0	37.0	37.0	33.0	37.0
13	35.283245306490244	37.0	37.0	37.0	33.0	37.0
14	35.25205536254921	37.0	37.0	37.0	33.0	37.0
15	35.274040365645874	37.0	37.0	37.0	33.0	37.0
16	35.289143592292625	37.0	37.0	37.0	33.0	37.0
17	35.24540218122277	37.0	37.0	37.0	33.0	37.0
18	35.22189409666384	37.0	37.0	37.0	33.0	37.0
19	35.26146763385441	37.0	37.0	37.0	33.0	37.0
20	35.16627637150429	37.0	37.0	37.0	33.0	37.0
21	35.24151872220642	37.0	37.0	37.0	33.0	37.0
22	35.27053169630872	37.0	37.0	37.0	33.0	37.0
23	35.23950921158605	37.0	37.0	37.0	33.0	37.0
24	35.22367767024356	37.0	37.0	37.0	33.0	37.0
25	35.240599025546835	37.0	37.0	37.0	33.0	37.0
26	35.17454832527491	37.0	37.0	37.0	33.0	37.0
27	35.14256095648452	37.0	37.0	37.0	33.0	37.0
28	35.12986861096565	37.0	37.0	37.0	33.0	37.0
29	35.16535135867904	37.0	37.0	37.0	33.0	37.0
30	35.1262748829779	37.0	37.0	37.0	33.0	37.0
31	35.117428783315745	37.0	37.0	37.0	33.0	37.0
32	35.1666352126865	37.0	37.0	37.0	33.0	37.0
33	35.14918489889982	37.0	37.0	37.0	33.0	37.0
34	35.07459377849133	37.0	37.0	37.0	33.0	37.0
35	35.086913992413834	37.0	37.0	37.0	33.0	37.0
36	35.063179970814254	37.0	37.0	37.0	27.0	37.0
37	35.07805460233752	37.0	37.0	37.0	33.0	37.0
38	34.936105004904164	37.0	37.0	37.0	27.0	37.0
39	34.99622552237973	37.0	37.0	37.0	27.0	37.0
40	35.03620308816063	37.0	37.0	37.0	27.0	37.0
41	35.06831007067842	37.0	37.0	37.0	33.0	37.0
42	34.933617039374184	37.0	37.0	37.0	27.0	37.0
43	34.766000994122976	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	12.0
16	17.0
17	20.0
18	18.0
19	15.0
20	22.0
21	75.0
22	205.0
23	477.0
24	1067.0
25	1864.0
26	3002.0
27	4560.0
28	6335.0
29	8672.0
30	11382.0
31	14787.0
32	18589.0
33	24340.0
34	35250.0
35	64202.0
36	181299.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.548256164758605	20.81172533498489	13.656697970022142	24.983320530234362
2	15.95088926161117	22.87944796935762	38.23625571820069	22.933407050830517
3	17.643822216787918	27.00080539909785	30.225857298165128	25.129515085949105
4	12.52089917626013	18.12493520923099	39.36833319599906	29.98583241850983
5	13.69311370480927	37.09992530787245	35.64515657436917	13.561804412949115
6	29.52811055498111	40.033651328642705	17.44074468848598	12.9974934278902
7	26.3955599384388	32.29676963193527	23.720465377142084	17.587205052483846
8	23.911315724420604	36.475275842545805	20.763082419174346	18.850326013859245
9	25.701800319501555	15.644412311176442	20.398925071303072	38.254862298018935
10	14.615468447227753	28.36573093290733	34.31000156826887	22.708799051596046
11	33.96817211617949	23.706377538136845	24.33235604487907	17.9930943008046
12	23.086512621906323	25.67123236694302	30.365938263368164	20.876316747782493
13	27.564850575873646	22.4320926288705	26.33841115756849	23.66464563768736
14	21.875490084021997	20.84521717865772	27.788926958541882	29.4903657787784
15	23.793031038433217	28.781455087703446	24.50247334607441	22.923040527788928
16	23.02750318305419	28.57917498425086	25.221218943624724	23.172102889070228
17	22.30742854408829	27.763940979928815	26.46360685891694	23.465023617065956
18	23.033085156999665	27.418124403592664	27.72646201200922	21.822328427398453
19	24.615176058116322	26.50028840198718	27.128127566711235	21.75640797318526
20	24.0537889641717	26.50321229310148	27.399252015491303	22.043746727235515
21	23.049033653986726	27.123343017615113	27.370278912631473	22.457344415766684
22	22.802895183819718	27.088256324243577	27.165074918064597	22.943773573872107
23	22.735911496474053	27.017551320934263	27.808330963209478	22.43820621938221
24	23.116548957898626	27.471817676782447	26.155535058783503	23.256098306535428
25	23.142066553077928	27.133975348939828	27.2626265579688	22.46133154001345
26	23.129839372054512	27.37054472091459	27.130519841259293	22.3690960657716
27	23.1192070407298	27.09277506505658	26.709745329083944	23.078272565129673
28	22.723684315450637	26.97289552937049	27.461982770307085	22.84143738487179
29	23.823598990991755	27.022335870030386	26.671468936314994	22.482596202662865
30	23.388205023244936	26.73021256688401	27.320306955405343	22.56127545446571
31	23.307930921743385	26.927973929523592	27.053701247438273	22.71039390129475
32	22.19897876457626	26.828827439920683	27.480855158408446	23.491338637094607
33	22.320718958244175	26.96678193885878	27.955057135490456	22.75744196740659
34	23.113359258501212	26.557171374574374	27.555813094247643	22.77365627267677
35	22.992150681399533	27.252260034927207	27.17065689201007	22.58493239166319
36	23.603775540853402	26.956415415817187	26.779387099260788	22.66042194406862
37	22.84382965941985	26.647811999117515	27.098888655568288	23.40946968589435
38	23.217556105483357	26.87002772380393	26.90697507515729	23.00544109555542
39	22.75265741831047	26.862319283593518	27.41546632076149	22.969556977334527
40	22.764087174484533	27.380113819106832	27.42237733612255	22.433421670286087
41	22.93925483305911	26.360207436784144	27.373202803745773	23.327334926410977
42	22.539213366966944	27.117495235386524	27.404833989436778	22.938457408209757
43	23.308994154875855	26.125498722791203	27.351140716247002	23.214366406085947
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	10.5
2	15.0
3	58.5
4	102.0
5	102.0
6	114.0
7	126.0
8	131.5
9	137.0
10	190.0
11	243.0
12	243.0
13	359.5
14	476.0
15	913.0
16	1350.0
17	1605.5
18	1861.0
19	1861.0
20	1824.0
21	1787.0
22	2235.5
23	2684.0
24	3664.0
25	4644.0
26	4644.0
27	6133.0
28	7622.0
29	9367.5
30	11113.0
31	13361.0
32	15609.0
33	15609.0
34	18162.0
35	20715.0
36	22689.0
37	24663.0
38	26448.0
39	28233.0
40	28233.0
41	29534.0
42	30835.0
43	31363.5
44	31892.0
45	31003.0
46	30114.0
47	30114.0
48	29082.5
49	28051.0
50	27842.5
51	27634.0
52	26117.5
53	24601.0
54	24601.0
55	23334.0
56	22067.0
57	19200.0
58	16333.0
59	15053.0
60	13773.0
61	13773.0
62	11949.5
63	10126.0
64	8422.0
65	6718.0
66	5681.0
67	4644.0
68	4644.0
69	3886.0
70	3128.0
71	2596.0
72	2064.0
73	1628.0
74	1192.0
75	1192.0
76	943.5
77	695.0
78	523.0
79	351.0
80	298.5
81	246.0
82	246.0
83	195.5
84	145.0
85	130.0
86	115.0
87	88.5
88	62.0
89	62.0
90	48.5
91	35.0
92	19.5
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	376211.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.34356743425346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.10937293629935	60.009430653597164
2	6.08427160472163	8.194731102017693
3	1.6973120235737356	3.4290914014952114
4	0.8311973557846886	2.2390318072183732
5	0.46572576514841296	1.568181723557072
6	0.31189246499606615	1.2602370749218872
7	0.22980016120210542	1.083289385662141
8	0.1630825846940753	0.8786050431758249
9	0.12740853171925223	0.7722130542781215
>10	0.8469114154630536	10.81017211573042
>50	0.08974234431641649	4.130218262018448
>100	0.042488632043032576	5.150404902922464
>500	3.9709001909376233E-4	0.19975869483297407
>1k	3.9709001909376233E-4	0.2746347785722415
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1027	0.2729851067618969	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	747	0.1985587874889357	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	480	0.1275879758965049	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	438	0.11642402800556072	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	385	0.10233618900032163	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.6580828311771853E-4	0.0	0.0
2	0.0	0.0	2.6580828311771853E-4	0.0	0.0
3	0.0	0.0	2.6580828311771853E-4	0.0	0.0
4	0.0	0.0	2.6580828311771853E-4	0.0	0.0
5	0.0	0.0	5.316165662354371E-4	0.0	0.0
6	0.0	0.0	0.0010632331324708741	0.0	0.0
7	0.0	0.0	0.0029238911142949035	0.0	0.0
8	0.0	0.0	0.0042529325298834965	0.0	0.0
9	0.0	0.0	0.004784549096118933	0.0	0.0
10	2.6580828311771853E-4	0.0	0.005581973945472089	0.0	0.0
11	2.6580828311771853E-4	0.0	0.008771673342884711	2.6580828311771853E-4	0.0
12	2.6580828311771853E-4	0.0	0.009834906475355586	2.6580828311771853E-4	0.0
13	2.6580828311771853E-4	0.0	0.010366523041591021	2.6580828311771853E-4	0.0
14	2.6580828311771853E-4	0.0	0.010898139607826459	2.6580828311771853E-4	0.0
15	2.6580828311771853E-4	0.0	0.011163947890944178	2.6580828311771853E-4	0.0
16	2.6580828311771853E-4	0.0	0.012227181023415051	2.6580828311771853E-4	2.6580828311771853E-4
17	2.6580828311771853E-4	0.0	0.012492989306532771	2.6580828311771853E-4	2.6580828311771853E-4
18	2.6580828311771853E-4	0.0	0.012492989306532771	2.6580828311771853E-4	2.6580828311771853E-4
19	2.6580828311771853E-4	0.0	0.013290414155885926	2.6580828311771853E-4	2.6580828311771853E-4
20	2.6580828311771853E-4	0.0	0.013556222439003644	5.316165662354371E-4	2.6580828311771853E-4
21	2.6580828311771853E-4	0.0	0.013822030722121363	7.974248493531555E-4	5.316165662354371E-4
22	2.6580828311771853E-4	0.0	0.0143536472883568	0.0010632331324708741	5.316165662354371E-4
23	2.6580828311771853E-4	0.0	0.0143536472883568	0.0013290414155885926	5.316165662354371E-4
24	2.6580828311771853E-4	0.0	0.0143536472883568	0.0018606579818240296	5.316165662354371E-4
25	2.6580828311771853E-4	0.0	0.0143536472883568	0.0021264662649417483	5.316165662354371E-4
26	2.6580828311771853E-4	0.0	0.0143536472883568	0.0023922745480594665	5.316165662354371E-4
27	2.6580828311771853E-4	0.0	0.0143536472883568	0.0023922745480594665	5.316165662354371E-4
28	2.6580828311771853E-4	0.0	0.0143536472883568	0.008240056776649273	5.316165662354371E-4
29	2.6580828311771853E-4	0.0	0.0143536472883568	0.018606579818240297	5.316165662354371E-4
30	2.6580828311771853E-4	0.0	0.0143536472883568	0.03721315963648059	5.316165662354371E-4
31	2.6580828311771853E-4	0.0	0.0143536472883568	0.09037481626002429	5.316165662354371E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACTCCG	25	0.0054917037	29.599998	21
GGTATCA	270	0.0	29.462963	1
GTATCAA	810	0.0	23.067902	1
GTATTAG	150	0.0	20.966667	1
TCGGGAT	85	1.2407581E-6	19.588234	22
AACGGCC	110	3.8311555E-8	18.5	37
TTAACGG	110	3.8311555E-8	18.5	35
GACTGTG	60	9.221078E-4	18.5	7
CGGGTCA	50	0.0070263045	18.499998	16
GTTTCGG	50	0.0070263045	18.499998	21
TCGGGGG	50	0.0070263045	18.499998	24
CCAAACG	50	0.0070263045	18.499998	29
GTATAGG	145	1.546141E-10	17.862068	1
ATTAGAG	115	6.3710104E-8	17.69565	3
CCATCGG	85	2.7152404E-5	17.411764	19
GCTATAT	75	2.062612E-4	17.266666	19
ACGGGTC	75	2.062612E-4	17.266666	15
TATTAGA	110	7.773324E-7	16.818182	2
TACCGCA	90	4.4331777E-5	16.444445	7
GGCACCG	125	1.6489139E-7	16.279999	9
>>END_MODULE