FastQCFastQC Report
Fri 10 Feb 2017
ERR1632862.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632862.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488030
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24880.5098047251193574No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17940.3676003524373502No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9350.1915865827920415No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA5490.11249308444153024No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC5360.10982931377169437No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT5210.10675573222957604No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG5210.10675573222957604No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC5150.10552629961272872No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.10060856914533942No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT251.2325846E-437.010
CCGGTTA405.9332335E-527.7523
AATCGCC405.9332335E-527.7518
CGCCGTT556.249338E-726.9090925
AGGACCG706.5720087E-926.428575
GGTATCA4400.026.0681821
GTTCGCC509.0770955E-625.90000224
TTACCGG601.3347726E-624.66666831
CGGTTAT451.3216882E-424.66666624
GCCGGTT451.3216882E-424.66666622
CGCCGGT451.3216882E-424.66666621
GTATCAA12200.024.5655731
TGTTACG400.001929603723.12516
ATAAGAC400.001929603723.1253
TTTACCG652.677596E-622.7692330
GTTACGG652.677596E-622.7692317
TACCGGG652.677596E-622.7692332
GGACCGT753.7316386E-722.26
TCGCCAT1107.4578566E-1121.86363613
GTCGCCA1107.4578566E-1121.86363612
Produced by FastQC (version 0.11.5)