##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1632862.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 488030 Sequences flagged as poor quality 0 Sequence length 43 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.769495727721658 33.0 33.0 33.0 27.0 33.0 2 31.277530069872753 33.0 33.0 33.0 27.0 33.0 3 31.72702907608139 33.0 33.0 33.0 27.0 33.0 4 31.84274532303342 33.0 33.0 33.0 27.0 33.0 5 31.956611273897096 33.0 33.0 33.0 33.0 33.0 6 34.8560641763826 37.0 37.0 37.0 27.0 37.0 7 35.032315636333834 37.0 37.0 37.0 33.0 37.0 8 35.21021248693728 37.0 37.0 37.0 33.0 37.0 9 35.29019117677192 37.0 37.0 37.0 33.0 37.0 10 35.332956990348954 37.0 37.0 37.0 33.0 37.0 11 35.37802799008258 37.0 37.0 37.0 33.0 37.0 12 35.314343380529884 37.0 37.0 37.0 33.0 37.0 13 35.342860070077656 37.0 37.0 37.0 33.0 37.0 14 35.3282605577526 37.0 37.0 37.0 33.0 37.0 15 35.347931479622154 37.0 37.0 37.0 33.0 37.0 16 35.33440567178247 37.0 37.0 37.0 33.0 37.0 17 35.30068028604799 37.0 37.0 37.0 33.0 37.0 18 35.30993176648977 37.0 37.0 37.0 33.0 37.0 19 35.30683154724095 37.0 37.0 37.0 33.0 37.0 20 35.33193246316825 37.0 37.0 37.0 33.0 37.0 21 35.316124008769954 37.0 37.0 37.0 33.0 37.0 22 35.31250128065898 37.0 37.0 37.0 33.0 37.0 23 35.31314673278282 37.0 37.0 37.0 33.0 37.0 24 35.3075138823433 37.0 37.0 37.0 33.0 37.0 25 35.29868245804561 37.0 37.0 37.0 33.0 37.0 26 35.258857037477206 37.0 37.0 37.0 33.0 37.0 27 35.19706165604573 37.0 37.0 37.0 33.0 37.0 28 35.18739216851423 37.0 37.0 37.0 33.0 37.0 29 35.20714095444952 37.0 37.0 37.0 33.0 37.0 30 35.185920947482735 37.0 37.0 37.0 33.0 37.0 31 35.17157346884413 37.0 37.0 37.0 33.0 37.0 32 35.19095547404873 37.0 37.0 37.0 33.0 37.0 33 35.16755937135012 37.0 37.0 37.0 33.0 37.0 34 35.107472901256074 37.0 37.0 37.0 33.0 37.0 35 35.09316640370469 37.0 37.0 37.0 33.0 37.0 36 35.08872405384915 37.0 37.0 37.0 33.0 37.0 37 35.09050263303485 37.0 37.0 37.0 33.0 37.0 38 34.9446263549372 37.0 37.0 37.0 27.0 37.0 39 35.0105833657767 37.0 37.0 37.0 27.0 37.0 40 35.04716718234535 37.0 37.0 37.0 27.0 37.0 41 35.04643362088396 37.0 37.0 37.0 27.0 37.0 42 34.94378419359466 37.0 37.0 37.0 27.0 37.0 43 34.72449644489068 37.0 37.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 9.0 16 24.0 17 31.0 18 19.0 19 29.0 20 39.0 21 68.0 22 210.0 23 579.0 24 1230.0 25 2178.0 26 3682.0 27 5589.0 28 8099.0 29 11219.0 30 14640.0 31 18450.0 32 23726.0 33 31671.0 34 44675.0 35 82507.0 36 239356.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.300329897752185 20.321291723869432 13.975985082884248 24.40239329549413 2 15.580189742433866 23.732967235620762 38.62221584738643 22.06462717455894 3 17.85033706944245 27.09485072638977 31.2312767657726 23.82353543839518 4 12.77114111837387 18.097453025428763 39.026084462020776 30.10532139417659 5 13.728049505153372 37.30057578427556 35.17980452021392 13.791570190357149 6 28.77405077556708 39.90000614716308 17.68395385529578 13.641989221974061 7 25.573018052168923 33.03854271253817 23.542405180009425 17.846034055283486 8 23.85304182119952 36.154539679937706 21.06612298424277 18.926295514620005 9 26.121959715591252 15.6564145646784 20.173349998975475 38.04827572075487 10 14.815482654754831 28.442923590762863 33.87168821588837 22.869905538593937 11 33.14058561973649 24.198717291969757 24.259369301067558 18.401327787226197 12 22.61377374341741 26.12585291887794 30.294244206298792 20.966129131405857 13 28.049710058807857 22.491445198041106 26.756756756756754 22.70208798639428 14 22.083888285556217 21.456467840091797 28.162203143249393 28.297440731102597 15 23.975780177448108 28.471610351822633 25.315042108067125 22.23756736266213 16 22.589594901952747 28.837571460770857 25.309304755855173 23.263528881421223 17 22.34493781120013 27.581706042661313 26.250435424051798 23.822920722086756 18 22.928508493330327 26.985021412618078 27.909964551359543 22.17650554269205 19 25.000512263590352 26.258221830625168 28.03065385324673 20.710612052537755 20 24.56324406286499 25.908858062004388 28.023687068417924 21.504210806712702 21 22.676884617748907 27.46572956580538 27.2739380775772 22.58344773886851 22 22.3900170071512 27.203040796672333 27.4290514927361 22.977890703440362 23 22.241255660512675 27.34381083130135 28.08700284818556 22.32793066000041 24 23.235251931233737 27.16902649427289 27.16841177796447 22.427309796528903 25 23.156773149191647 26.681556461692928 28.027375366268465 22.134295022846956 26 23.048173268036802 26.974366329938732 27.32311538225109 22.654345019773377 27 22.918058316087127 26.77110013728664 27.483966149621946 22.826875397004283 28 22.083478474683936 27.226604921828578 27.958527139725014 22.731389463762476 29 23.146322971948447 27.324754625740223 27.1665676290392 22.362354773272138 30 22.838964817736613 27.34258139868451 27.571870581726532 22.246583201852346 31 22.919082843267834 26.874987193410245 27.759768866668033 22.446161096653896 32 21.88635944511608 26.87683134233551 27.948281867917956 23.288527344630452 33 22.17896440792574 26.53238530418212 28.324078437800953 22.964571850091183 34 22.21707681904801 26.77110013728664 28.108927729852674 22.902895313812678 35 22.522385918898426 27.18500911829191 27.684363666168068 22.608241296641598 36 22.772165645554576 27.130504272278344 27.639694281089277 22.457635801077803 37 23.181566706964734 26.49427289305985 27.476589553920867 22.847570846054545 38 22.97912013605721 26.91187017191566 27.4991291518964 22.60988054013073 39 22.536934204864455 26.766797123127677 27.83107595844518 22.865192713562692 40 22.38981210171506 27.05264020654468 28.082904739462737 22.474642952277524 41 22.359486097166158 26.578693932750035 28.199905743499375 22.86191422658443 42 22.505173862262566 26.78257484171055 27.71018175112186 23.002069544905027 43 22.393910210437884 26.480544228838394 27.874925721779398 23.250619838944328 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 12.0 1 23.0 2 34.0 3 129.5 4 225.0 5 225.0 6 288.0 7 351.0 8 344.0 9 337.0 10 477.5 11 618.0 12 618.0 13 999.0 14 1380.0 15 2456.5 16 3533.0 17 3924.0 18 4315.0 19 4315.0 20 3935.5 21 3556.0 22 3776.0 23 3996.0 24 5307.0 25 6618.0 26 6618.0 27 8399.5 28 10181.0 29 13435.5 30 16690.0 31 18653.0 32 20616.0 33 20616.0 34 23027.0 35 25438.0 36 27282.5 37 29127.0 38 31542.5 39 33958.0 40 33958.0 41 36175.0 42 38392.0 43 37116.5 44 35841.0 45 36549.5 46 37258.0 47 37258.0 48 36868.0 49 36478.0 50 37358.0 51 38238.0 52 37977.5 53 37717.0 54 37717.0 55 33037.5 56 28358.0 57 25225.0 58 22092.0 59 19383.5 60 16675.0 61 16675.0 62 14530.0 63 12385.0 64 10235.0 65 8085.0 66 6903.5 67 5722.0 68 5722.0 69 4703.0 70 3684.0 71 3133.5 72 2583.0 73 2153.5 74 1724.0 75 1724.0 76 1261.0 77 798.0 78 626.5 79 455.0 80 344.5 81 234.0 82 234.0 83 180.5 84 127.0 85 113.0 86 99.0 87 80.5 88 62.0 89 62.0 90 46.0 91 30.0 92 18.5 93 7.0 94 3.5 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 488030.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.09656287354166 #Duplication Level Percentage of deduplicated Percentage of total 1 90.25232960844592 56.043594600144665 2 5.4050463855735185 6.7126960543235015 3 1.400585886228235 2.6091470863189996 4 0.631267965511406 1.5679828364172699 5 0.3899840030507157 1.210833308255711 6 0.2701018391461227 1.0063437502077865 7 0.19136800963780398 0.8318306949720895 8 0.15060029601028013 0.7481408599981075 9 0.127726938969427 0.7138263506724037 >10 0.9573076768649836 12.351818150428057 >50 0.14213088367050067 6.264485691642902 >100 0.0788876331967184 8.31489145102272 >500 0.0019971552708029977 0.7393449020659311 >1k 6.657184236009992E-4 0.8850642635298621 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 2488 0.5098047251193574 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 1794 0.3676003524373502 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 935 0.1915865827920415 No Hit ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA 549 0.11249308444153024 No Hit GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC 536 0.10982931377169437 No Hit GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT 521 0.10675573222957604 No Hit GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG 521 0.10675573222957604 No Hit GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC 515 0.10552629961272872 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 491 0.10060856914533942 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.001024527180706104 0.0 0.0 2 0.0 0.0 0.001024527180706104 0.0 0.0 3 0.0 0.0 0.0014343380529885457 0.0 0.0 4 0.0 0.0 0.0016392434891297667 0.0 0.0 5 0.0 0.0 0.0026637706698358706 0.0 0.0 6 0.0 0.0 0.0049177304673892995 0.0 0.0 7 0.0 0.0 0.009630555498637379 2.0490543614122084E-4 0.0 8 0.0 0.0 0.011884515296190808 2.0490543614122084E-4 0.0 9 0.0 0.0 0.014548285966026678 6.147163084236624E-4 0.0 10 0.001024527180706104 0.0 0.017007151199721328 6.147163084236624E-4 0.0 11 0.001024527180706104 0.0 0.02438374690080528 8.196217445648834E-4 0.0 12 0.0012294326168473249 0.0 0.028481855623629693 8.196217445648834E-4 0.0 13 0.0012294326168473249 0.0 0.030530909985041904 8.196217445648834E-4 0.0 14 0.0012294326168473249 0.0 0.03237505891031289 8.196217445648834E-4 0.0 15 0.0012294326168473249 0.0 0.03708788394156097 8.196217445648834E-4 0.0 16 0.0012294326168473249 0.0 0.04057127635596172 0.0012294326168473249 0.0 17 0.0012294326168473249 0.0 0.04139089810052661 0.0012294326168473249 0.0 18 0.0012294326168473249 0.0 0.04262033071737393 0.0012294326168473249 0.0 19 0.0012294326168473249 0.0 0.04343995246193882 0.0012294326168473249 0.0 20 0.0012294326168473249 0.0 0.0442595742065037 0.0014343380529885457 0.0 21 0.0012294326168473249 0.0 0.045489006823351025 0.0014343380529885457 0.0 22 0.0012294326168473249 2.0490543614122084E-4 0.04569391225949224 0.002253959797553429 0.0 23 0.0012294326168473249 2.0490543614122084E-4 0.04589881769563346 0.0032784869782595334 0.0 24 0.0012294326168473249 2.0490543614122084E-4 0.04610372313177469 0.004098108722824416 0.0 25 0.0012294326168473249 2.0490543614122084E-4 0.04610372313177469 0.0043030141589656375 0.0 26 0.0012294326168473249 2.0490543614122084E-4 0.04610372313177469 0.004712825031248079 0.0 27 0.0012294326168473249 2.0490543614122084E-4 0.04610372313177469 0.005942257648095404 0.0 28 0.0012294326168473249 2.0490543614122084E-4 0.04610372313177469 0.01762186750814499 0.0 29 0.0012294326168473249 2.0490543614122084E-4 0.04610372313177469 0.04221051984509149 0.0 30 0.0012294326168473249 2.0490543614122084E-4 0.04610372313177469 0.07294633526627461 0.0 31 0.0012294326168473249 2.0490543614122084E-4 0.04610372313177469 0.16843226850808352 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTATT 25 1.2325846E-4 37.0 10 CCGGTTA 40 5.9332335E-5 27.75 23 AATCGCC 40 5.9332335E-5 27.75 18 CGCCGTT 55 6.249338E-7 26.90909 25 AGGACCG 70 6.5720087E-9 26.42857 5 GGTATCA 440 0.0 26.068182 1 GTTCGCC 50 9.0770955E-6 25.900002 24 TTACCGG 60 1.3347726E-6 24.666668 31 CGGTTAT 45 1.3216882E-4 24.666666 24 GCCGGTT 45 1.3216882E-4 24.666666 22 CGCCGGT 45 1.3216882E-4 24.666666 21 GTATCAA 1220 0.0 24.565573 1 TGTTACG 40 0.0019296037 23.125 16 ATAAGAC 40 0.0019296037 23.125 3 TTTACCG 65 2.677596E-6 22.76923 30 GTTACGG 65 2.677596E-6 22.76923 17 TACCGGG 65 2.677596E-6 22.76923 32 GGACCGT 75 3.7316386E-7 22.2 6 TCGCCAT 110 7.4578566E-11 21.863636 13 GTCGCCA 110 7.4578566E-11 21.863636 12 >>END_MODULE