FastQCFastQC Report
Fri 10 Feb 2017
ERR1632861.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632861.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483815
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14860.30714219278029825No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10600.21909200830896106No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5690.11760693653565929No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC5540.11450657792751362No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5350.11057945702386243No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG5140.10623895497245849No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG5030.10396535865981832No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAT250.00549323729.610
CGACTTA250.00549323729.612
GGTATCA3650.023.8219181
GTATCAA9600.022.9322911
CAGTCCG450.003822654720.55555521
AGCTTCG1303.274181E-1119.92307721
GTGTAAG951.6711056E-719.4736841
AACGGCC1254.110916E-1019.2437
TTAACGG1254.110916E-1019.2435
CGCTCTC1355.638867E-1119.18518629
CGGGTCA701.2173463E-418.56
GCGGTGA500.00702913918.520
TTCAACG609.2262815E-418.519
CCGCTCT1409.276846E-1118.528
AACGATT701.2173463E-418.522
GTCCGAC500.00702913918.523
CAATCTA1103.8378857E-818.534
TTCGCCG1409.276846E-1118.524
TTAGGAC1552.0008883E-1117.9032253
GCTTCGC1451.546141E-1017.86206822
Produced by FastQC (version 0.11.5)