##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632861.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	483815
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78590576976737	33.0	33.0	33.0	33.0	33.0
2	31.286179634777756	33.0	33.0	33.0	27.0	33.0
3	31.735874249454852	33.0	33.0	33.0	27.0	33.0
4	31.86222833107696	33.0	33.0	33.0	33.0	33.0
5	31.961173175697322	33.0	33.0	33.0	33.0	33.0
6	34.872085404545125	37.0	37.0	37.0	27.0	37.0
7	35.0486446265618	37.0	37.0	37.0	33.0	37.0
8	35.23544950032554	37.0	37.0	37.0	33.0	37.0
9	35.30255366204024	37.0	37.0	37.0	33.0	37.0
10	35.34376982937693	37.0	37.0	37.0	33.0	37.0
11	35.367723199983466	37.0	37.0	37.0	33.0	37.0
12	35.3349648109298	37.0	37.0	37.0	33.0	37.0
13	35.36251459752178	37.0	37.0	37.0	33.0	37.0
14	35.320310449241966	37.0	37.0	37.0	33.0	37.0
15	35.34940834823228	37.0	37.0	37.0	33.0	37.0
16	35.33696144187344	37.0	37.0	37.0	33.0	37.0
17	35.31580046091998	37.0	37.0	37.0	33.0	37.0
18	35.323280592788564	37.0	37.0	37.0	33.0	37.0
19	35.31651354339985	37.0	37.0	37.0	33.0	37.0
20	35.333470438080674	37.0	37.0	37.0	33.0	37.0
21	35.308299659994006	37.0	37.0	37.0	33.0	37.0
22	35.325349565433065	37.0	37.0	37.0	33.0	37.0
23	35.325651333670926	37.0	37.0	37.0	33.0	37.0
24	35.305594080381965	37.0	37.0	37.0	33.0	37.0
25	35.30127631429369	37.0	37.0	37.0	33.0	37.0
26	35.261924495933364	37.0	37.0	37.0	33.0	37.0
27	35.22059671568678	37.0	37.0	37.0	33.0	37.0
28	35.19133139733162	37.0	37.0	37.0	33.0	37.0
29	35.242522451763584	37.0	37.0	37.0	33.0	37.0
30	35.206581027872225	37.0	37.0	37.0	33.0	37.0
31	35.176565422733894	37.0	37.0	37.0	33.0	37.0
32	35.20978473176731	37.0	37.0	37.0	33.0	37.0
33	35.19529778944431	37.0	37.0	37.0	33.0	37.0
34	35.134880067794505	37.0	37.0	37.0	33.0	37.0
35	35.12527308992073	37.0	37.0	37.0	33.0	37.0
36	35.116670628236	37.0	37.0	37.0	33.0	37.0
37	35.14424935150832	37.0	37.0	37.0	33.0	37.0
38	34.99157322530306	37.0	37.0	37.0	27.0	37.0
39	35.06691400638674	37.0	37.0	37.0	27.0	37.0
40	35.09633847648378	37.0	37.0	37.0	33.0	37.0
41	35.12553145313808	37.0	37.0	37.0	33.0	37.0
42	35.041108688238275	37.0	37.0	37.0	27.0	37.0
43	34.815580335458804	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	15.0
16	24.0
17	19.0
18	26.0
19	22.0
20	32.0
21	82.0
22	225.0
23	559.0
24	1204.0
25	2160.0
26	3650.0
27	5447.0
28	7660.0
29	10746.0
30	14002.0
31	18254.0
32	23505.0
33	31147.0
34	44150.0
35	82261.0
36	238623.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.12915060508666	20.35881483624939	13.362958982255616	25.149075576408336
2	16.058410756177466	22.869691927699638	37.843803933321624	23.228093382801276
3	17.712348728336245	26.903878548618792	30.148713867904053	25.235058855140913
4	12.8170891766481	17.951903103458967	39.13706685406612	30.093940865826813
5	14.172152578981636	36.64479191426475	35.40382170871098	13.77923379804264
6	29.540836890133626	39.81232495892025	17.355600797825616	13.291237353120511
7	26.730878538284266	31.896282670028835	23.643128055145045	17.72971073654186
8	24.377086282980066	35.9172410942199	20.70316133232744	19.002511290472597
9	26.168266796192757	15.279600673811272	20.017362008205616	38.53477052179035
10	15.152072589729546	27.82861217614171	34.00576666701115	23.0135485671176
11	34.4191478147639	23.503198536630737	23.93394169258911	18.143711956016244
12	23.211144755743415	25.377468660541737	30.007337515372612	21.40404906834224
13	28.052458067649827	22.17335138431012	26.00456786168267	23.76962268635739
14	22.298605872079204	20.718663125368167	27.516716100162252	29.466014902390373
15	24.253278629228113	28.331076961235187	24.36344470510422	23.05219970443248
16	23.014168638839223	28.14381530130318	25.138947738288397	23.703068321569194
17	22.517697880388166	27.243677852071556	26.224900013434887	24.01372425410539
18	23.252276179944815	26.982627657265688	27.374926366483056	22.390169796306438
19	24.98909707222802	26.173640751113545	26.869154532207556	21.968107644450875
20	24.4800181887705	26.073395822783503	27.185804491386172	22.260781497059824
21	23.227059929931894	27.00309002407945	27.050215474923267	22.719634571065388
22	23.304775585709415	26.64716885586433	26.888376755578065	23.159678802848198
23	23.05716027820551	26.642208282091296	27.518576315327138	22.78205512437605
24	23.307669253743683	27.19737916352325	26.054173599412998	23.44077798332007
25	23.543296507962754	26.699874952202805	27.13557868193421	22.62124985790023
26	23.414734971011647	26.92847472691008	26.877835536310368	22.778954765767907
27	23.39902648739704	26.82554282111964	26.514266816861813	23.2611638746215
28	22.962909376517885	26.659776980870788	27.339788968924072	23.03752467368726
29	24.043281006169714	26.608311027975567	26.446885689778117	22.901522276076598
30	23.795665698665815	26.26603143763629	27.02045203228507	22.917850831412835
31	23.543916579684385	26.473755464382048	26.93756911216064	23.044758843772932
32	22.632824530037308	26.375577441790764	27.272614532414245	23.718983495757676
33	22.63778510381034	26.49359775947418	27.64920475801701	23.219412378698472
34	23.354174632865867	26.040945402684912	27.56425493215382	23.040625032295402
35	23.141490032347075	26.847865403098293	27.029753108109507	22.98089145644513
36	23.784091026528735	26.643861806682306	26.63001353823259	22.94203362855637
37	23.231193741409424	26.1407769498672	27.024379153188722	23.603650155534655
38	23.277285739383853	26.639727995204776	26.676312226780897	23.406674038630467
39	22.80065727602493	26.56035881483625	27.395388733296816	23.243595175842007
40	22.896355011729693	27.013011171625518	27.508035096059448	22.58259872058535
41	23.136736149147918	25.924165228444757	27.23417008567324	23.704928536734084
42	22.6844971735064	27.042774614263713	27.084112729038996	23.188615483190887
43	23.608197348159937	25.834048138234657	27.079358845839835	23.47839566776557
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	12.5
2	19.0
3	80.0
4	141.0
5	141.0
6	190.5
7	240.0
8	220.0
9	200.0
10	277.5
11	355.0
12	355.0
13	557.0
14	759.0
15	1319.0
16	1879.0
17	2157.5
18	2436.0
19	2436.0
20	2184.0
21	1932.0
22	2151.5
23	2371.0
24	3359.5
25	4348.0
26	4348.0
27	5998.0
28	7648.0
29	9922.5
30	12197.0
31	15156.0
32	18115.0
33	18115.0
34	21679.5
35	25244.0
36	27967.5
37	30691.0
38	33493.5
39	36296.0
40	36296.0
41	38125.0
42	39954.0
43	40739.0
44	41524.0
45	40858.0
46	40192.0
47	40192.0
48	38804.5
49	37417.0
50	37172.5
51	36928.0
52	34820.0
53	32712.0
54	32712.0
55	31070.5
56	29429.0
57	25746.5
58	22064.0
59	20301.0
60	18538.0
61	18538.0
62	16240.0
63	13942.0
64	11521.0
65	9100.0
66	7705.5
67	6311.0
68	6311.0
69	5276.0
70	4241.0
71	3488.5
72	2736.0
73	2238.5
74	1741.0
75	1741.0
76	1316.5
77	892.0
78	706.0
79	520.0
80	426.0
81	332.0
82	332.0
83	262.0
84	192.0
85	150.5
86	109.0
87	78.5
88	48.0
89	48.0
90	31.5
91	15.0
92	8.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	483815.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.30671505514245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.49019524699932	59.33800876471847
2	6.090911925527	8.077367229437757
3	1.601771083764756	3.186245365042691
4	0.7169827064674202	1.9016307206880028
5	0.41381422672063667	1.3719331008464684
6	0.27452514902404496	1.0921716499084717
7	0.18576268280464495	0.862211929362448
8	0.1523539111680534	0.8081669900285282
9	0.11794550324448871	0.7038520988100923
>10	0.8022140241530831	10.399958661078326
>50	0.09638389965239166	4.513182632055978
>100	0.05494196199434118	6.658401558723311
>500	0.001569770342695462	0.556861784260899
>1k	6.279081370781848E-4	0.5300075150385977
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1486	0.30714219278029825	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1060	0.21909200830896106	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	569	0.11760693653565929	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	554	0.11450657792751362	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	535	0.11057945702386243	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	514	0.10623895497245849	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	503	0.10396535865981832	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0669057387637837E-4	0.0	2.0669057387637837E-4	0.0	0.0
2	2.0669057387637837E-4	0.0	2.0669057387637837E-4	0.0	0.0
3	2.0669057387637837E-4	0.0	2.0669057387637837E-4	0.0	0.0
4	2.0669057387637837E-4	0.0	2.0669057387637837E-4	2.0669057387637837E-4	0.0
5	2.0669057387637837E-4	0.0	0.0010334528693818918	2.0669057387637837E-4	0.0
6	2.0669057387637837E-4	0.0	0.0028936680342692973	2.0669057387637837E-4	0.0
7	2.0669057387637837E-4	0.0	0.005994026642414972	4.1338114775275674E-4	0.0
8	2.0669057387637837E-4	0.0	0.007647551233426	4.1338114775275674E-4	0.0
9	2.0669057387637837E-4	0.0	0.009301075824437026	6.200717216291351E-4	0.0
10	2.0669057387637837E-4	0.0	0.009921147546066161	6.200717216291351E-4	0.0
11	2.0669057387637837E-4	0.0	0.015501793040728377	6.200717216291351E-4	0.0
12	2.0669057387637837E-4	0.0	0.01632855533623389	6.200717216291351E-4	0.0
13	2.0669057387637837E-4	0.0	0.017568698779492162	6.200717216291351E-4	0.0
14	2.0669057387637837E-4	0.0	0.02025567623988508	6.200717216291351E-4	0.0
15	2.0669057387637837E-4	0.0	0.021909200830896106	6.200717216291351E-4	0.0
16	2.0669057387637837E-4	0.0	0.023769415995783513	6.200717216291351E-4	0.0
17	2.0669057387637837E-4	0.0	0.024389487717412648	6.200717216291351E-4	0.0
18	2.0669057387637837E-4	0.0	0.024596178291289027	6.200717216291351E-4	0.0
19	2.0669057387637837E-4	0.0	0.024802868865165403	6.200717216291351E-4	0.0
20	2.0669057387637837E-4	0.0	0.026043012308423673	0.0010334528693818918	0.0
21	2.0669057387637837E-4	0.0	0.02645639345617643	0.0012401434432582701	0.0
22	2.0669057387637837E-4	0.0	0.02666308403005281	0.0014468340171346487	0.0
23	2.0669057387637837E-4	0.0	0.02666308403005281	0.001653524591011027	0.0
24	2.0669057387637837E-4	0.0	0.026869774603929187	0.0018602151648874053	0.0
25	4.1338114775275674E-4	0.0	0.027076465177805566	0.0018602151648874053	0.0
26	4.1338114775275674E-4	0.0	0.027076465177805566	0.0024802868865165402	0.0
27	4.1338114775275674E-4	0.0	0.027076465177805566	0.003927120903651189	0.0
28	4.1338114775275674E-4	0.0	0.027283155751681946	0.013021506154211836	0.0
29	4.1338114775275674E-4	0.0	0.027283155751681946	0.028109918047187456	0.0
30	4.1338114775275674E-4	0.0	0.027283155751681946	0.05187933404297097	0.0
31	4.1338114775275674E-4	0.0	0.02748984632555832	0.12401434432582702	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCAT	25	0.005493237	29.6	10
CGACTTA	25	0.005493237	29.6	12
GGTATCA	365	0.0	23.821918	1
GTATCAA	960	0.0	22.932291	1
CAGTCCG	45	0.0038226547	20.555555	21
AGCTTCG	130	3.274181E-11	19.923077	21
GTGTAAG	95	1.6711056E-7	19.473684	1
AACGGCC	125	4.110916E-10	19.24	37
TTAACGG	125	4.110916E-10	19.24	35
CGCTCTC	135	5.638867E-11	19.185186	29
CGGGTCA	70	1.2173463E-4	18.5	6
GCGGTGA	50	0.007029139	18.5	20
TTCAACG	60	9.2262815E-4	18.5	19
CCGCTCT	140	9.276846E-11	18.5	28
AACGATT	70	1.2173463E-4	18.5	22
GTCCGAC	50	0.007029139	18.5	23
CAATCTA	110	3.8378857E-8	18.5	34
TTCGCCG	140	9.276846E-11	18.5	24
TTAGGAC	155	2.0008883E-11	17.903225	3
GCTTCGC	145	1.546141E-10	17.862068	22
>>END_MODULE