FastQCFastQC Report
Fri 10 Feb 2017
ERR1632860.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632860.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411940
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74001.7963781133174734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT55281.341943001407972No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28450.6906345584308394No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA17470.4240908870223819No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG17020.4131669660630189No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG15490.3760256348011846No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15020.3646162062436277No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14330.3478661941059378No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT12270.29785891149196486No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12210.2964023886973831No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG10870.263873379618391No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9570.23231538573578675No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9200.2233334951691994No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC8850.21483711220080595No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8710.21143855901344855No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8590.2085255134242851No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8180.19857260766130988No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA8180.19857260766130988No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8090.19638782346943728No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7930.192503762683886No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC7770.1886197018983347No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7760.18837694809923777No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7620.18497839491188037No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7580.18400737971549255No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7290.17696751954168083No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7290.17696751954168083No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7270.17648201194348692No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7260.17623925814438995No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA7260.17623925814438995No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT7150.17356896635432342No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC7000.1699276593678691No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT6970.16919939797057823No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT6810.16531533718502694No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6230.1512356168374035No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA6200.15050735544011262No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA6070.1473515560518522No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5990.14540952565907656No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5850.14201097247171918No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5660.13739865028887702No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5650.13715589648978008No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT5590.13569937369519833No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5580.13545661989610136No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5180.12574646793222313No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC5110.12404719133854444No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5080.12331892994125358No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5040.12234791474486577No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5010.1216196533475749No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA5000.12137689954847793No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4990.12113414574938099No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4920.11943486915570228No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4870.1182211001602175No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4830.11725008496382969No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4830.11725008496382969No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT4800.11652182356653883No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4800.11652182356653883No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4760.11555080837015098No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4750.11530805457105404No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4710.11433703937466622No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4580.11118123998640579No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA4440.1077826867990484No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG4400.10681167160266059No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4360.10584065640627276No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4340.10535514880807885No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4250.10317036461620624No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC4250.10317036461620624No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4230.10268485701801233No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4150.10074282662523668No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACATCG250.00549230229.59999821
CACTACT250.00549230229.5999984
GATATCA405.931019E-527.7500021
GGTATCA11800.026.6525421
ATAGTAC358.8596955E-426.4285723
ACCATCG509.0726135E-625.89999818
GTATCAA32550.023.700461
CGGGCCG551.8988185E-523.54545417
TACAGTG400.001929095123.1250025
TCGCTGT502.6980264E-422.19999925
CTCGCTG502.6980264E-422.19999924
ACTCGCT502.6980264E-422.19999923
AACTCGC502.6980264E-422.19999922
TCCGCCA603.7188194E-521.58333212
AGCTTCT603.7188194E-521.58333237
ATACTAG1550.021.4838716
AAATCCG705.0888175E-621.14285930
CATACTA1600.020.81255
ACTAGTG1251.8189894E-1120.728
TCATACT1700.020.6764724
Produced by FastQC (version 0.11.5)