##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632860.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	411940
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.82573433024227	33.0	33.0	33.0	33.0	33.0
2	31.31467932223139	33.0	33.0	33.0	27.0	33.0
3	31.75991649269311	33.0	33.0	33.0	27.0	33.0
4	31.87132592125067	33.0	33.0	33.0	33.0	33.0
5	31.9894353546633	33.0	33.0	33.0	33.0	33.0
6	34.83635723649075	37.0	37.0	37.0	27.0	37.0
7	35.02411030732631	37.0	37.0	37.0	33.0	37.0
8	35.230543283002376	37.0	37.0	37.0	33.0	37.0
9	35.32563237364665	37.0	37.0	37.0	33.0	37.0
10	35.31332718357042	37.0	37.0	37.0	33.0	37.0
11	35.346717968636206	37.0	37.0	37.0	33.0	37.0
12	35.2881997378259	37.0	37.0	37.0	33.0	37.0
13	35.33146089236296	37.0	37.0	37.0	33.0	37.0
14	35.30885080351508	37.0	37.0	37.0	33.0	37.0
15	35.34520804000583	37.0	37.0	37.0	33.0	37.0
16	35.34582220711754	37.0	37.0	37.0	33.0	37.0
17	35.317143273292224	37.0	37.0	37.0	33.0	37.0
18	35.318548817789	37.0	37.0	37.0	33.0	37.0
19	35.304978880419476	37.0	37.0	37.0	33.0	37.0
20	35.306930620964216	37.0	37.0	37.0	33.0	37.0
21	35.29688546875759	37.0	37.0	37.0	33.0	37.0
22	35.28850318007477	37.0	37.0	37.0	33.0	37.0
23	35.27626838860028	37.0	37.0	37.0	33.0	37.0
24	35.285789192600866	37.0	37.0	37.0	33.0	37.0
25	35.262290624848276	37.0	37.0	37.0	33.0	37.0
26	35.2203500509783	37.0	37.0	37.0	33.0	37.0
27	35.15814924503569	37.0	37.0	37.0	33.0	37.0
28	35.109948050686995	37.0	37.0	37.0	33.0	37.0
29	35.16114239937855	37.0	37.0	37.0	33.0	37.0
30	35.098057969607225	37.0	37.0	37.0	33.0	37.0
31	35.08122542117784	37.0	37.0	37.0	33.0	37.0
32	35.09527115599359	37.0	37.0	37.0	33.0	37.0
33	35.04190173326212	37.0	37.0	37.0	27.0	37.0
34	34.957396708258486	37.0	37.0	37.0	27.0	37.0
35	34.91540515609069	37.0	37.0	37.0	27.0	37.0
36	34.87681943972423	37.0	37.0	37.0	27.0	37.0
37	34.86210370442297	37.0	37.0	37.0	27.0	37.0
38	34.66501189493616	37.0	37.0	37.0	27.0	37.0
39	34.68780890420935	37.0	37.0	37.0	27.0	37.0
40	34.650521920668055	37.0	37.0	37.0	27.0	37.0
41	34.6047579744623	37.0	37.0	37.0	27.0	37.0
42	34.43164781278827	37.0	37.0	37.0	27.0	37.0
43	34.18213332038646	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	13.0
16	21.0
17	35.0
18	24.0
19	16.0
20	36.0
21	75.0
22	213.0
23	506.0
24	1102.0
25	1977.0
26	3231.0
27	4987.0
28	7267.0
29	9909.0
30	13166.0
31	16773.0
32	21549.0
33	27538.0
34	39373.0
35	70201.0
36	193927.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.6189250861776	17.701364276350926	13.70418022042045	21.975530417051026
2	16.99592173617517	24.49191629849007	37.71544399669855	20.79671796863621
3	20.1114239937855	28.20265087148614	29.729329514006896	21.956595620721465
4	12.200563188813906	17.831965820265086	39.35573141719668	30.61173957372433
5	15.940185463902509	34.91090935573142	31.204544351119097	17.94436082924698
6	27.103218915376026	40.652764965771716	16.18123998640579	16.062776132446473
7	25.68432295965432	33.30873428169151	20.498373549546052	20.50856920910812
8	25.575326503859785	32.10734572996067	19.753847647715688	22.563480118463854
9	28.764140408797395	12.46977715201243	18.238092926154295	40.52798951303588
10	17.604262756712142	25.738457056852944	29.821090450065547	26.836189736369377
11	36.3082002233335	24.078506578627955	20.512210516094576	19.101082681943975
12	24.81890566587367	25.51318153129096	28.338107491382242	21.329805311453125
13	32.72952371704617	22.333592270719034	24.04622032334806	20.890663688886733
14	24.43219886391222	20.88605136670389	28.913676749041123	25.76807302034277
15	27.409574209836386	25.036898577462736	26.20575812011458	21.3477690925863
16	21.158421129290673	27.343302422682914	25.510268485701804	25.98800796232461
17	22.49502354711851	24.33970966645628	25.106568917803564	28.058697868621646
18	26.071029761615772	21.36670388891586	28.18274505996019	24.37952128950818
19	28.20313637908433	22.78559984463757	28.207020439869883	20.804243336408216
20	28.274748749817935	21.239743651988157	27.848473078603682	22.637034519590234
21	25.047579744623004	24.621789581006944	27.86061076855853	22.470019905811526
22	24.187745788221584	24.16759722289654	28.5488177889984	23.09583919988348
23	23.705393989415935	24.562800407826384	29.202068262368307	22.529737340389378
24	23.64980336942273	23.353400980725347	29.27829295528475	23.71850269456717
25	23.76875273098024	24.617662766422292	30.11069573238821	21.502888770209253
26	24.471767733165024	24.441666262077003	28.084187017526823	23.00237898723115
27	24.565956207214644	23.808807107831235	30.011409428557556	21.61382725639656
28	23.113074719619362	24.887119483419916	28.10069427586542	23.899111521095307
29	23.671165703743263	24.46424236539302	27.648201194348694	24.216390736515024
30	24.620818565810552	24.36592707675875	29.049133368937223	21.96412098849347
31	24.555517793853472	24.11079283390785	27.859396999563042	23.47429237267563
32	21.328106034859445	24.425159003738408	29.664757003447107	24.581977957955043
33	22.058552216342186	24.574695343982135	29.8725542554741	23.494198184201583
34	21.628877991940573	24.34529300383551	29.372481429334368	24.653347574889548
35	22.523425741612858	25.190318978492016	28.704908481817743	23.58134679807739
36	23.08540078652231	24.8883332524154	29.224887119483423	22.80137884157887
37	24.066368888673107	25.008253629169296	28.48060397145215	22.444773510705442
38	23.020342768364323	25.242996552896052	27.643588872165846	24.093071806573775
39	22.754041850754962	23.729426615526535	28.916832548429383	24.59969898528912
40	22.793125212409574	25.421906102830512	29.057144244307427	22.727824440452494
41	24.203039277564695	24.1707530222848	28.07180657377288	23.554401126377627
42	21.989124629800454	25.756663591785212	29.087973976792735	23.166237801621595
43	22.43482060494247	25.534543865611496	28.352187211729863	23.67844831771617
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5.0
1	44.0
2	83.0
3	395.5
4	708.0
5	708.0
6	889.0
7	1070.0
8	1089.0
9	1108.0
10	1495.5
11	1883.0
12	1883.0
13	3012.0
14	4141.0
15	6762.5
16	9384.0
17	9992.5
18	10601.0
19	10601.0
20	8160.0
21	5719.0
22	4175.5
23	2632.0
24	2591.5
25	2551.0
26	2551.0
27	2768.5
28	2986.0
29	3368.0
30	3750.0
31	4461.5
32	5173.0
33	5173.0
34	7189.5
35	9206.0
36	9479.5
37	9753.0
38	11385.5
39	13018.0
40	13018.0
41	15919.0
42	18820.0
43	21751.5
44	24683.0
45	29263.5
46	33844.0
47	33844.0
48	37724.0
49	41604.0
50	42509.0
51	43414.0
52	43816.0
53	44218.0
54	44218.0
55	39774.0
56	35330.0
57	32150.0
58	28970.0
59	25893.5
60	22817.0
61	22817.0
62	18291.0
63	13765.0
64	10426.5
65	7088.0
66	6497.0
67	5906.0
68	5906.0
69	4859.5
70	3813.0
71	2822.5
72	1832.0
73	1686.5
74	1541.0
75	1541.0
76	927.0
77	313.0
78	204.0
79	95.0
80	77.0
81	59.0
82	59.0
83	47.5
84	36.0
85	23.5
86	11.0
87	8.5
88	6.0
89	6.0
90	5.0
91	4.0
92	2.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	411940.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.402234027996954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.30867724710322	32.76043788262117
2	6.995893431740227	5.373158736012298
3	2.1874040263183177	2.520036039973766
4	1.0997648706551215	1.6893371175467113
5	0.6999879456760469	1.3440550453314186
6	0.48420087643016074	1.1156637223939359
7	0.35240598255645794	0.9473223910499518
8	0.27613724918699944	0.8483429813701173
9	0.22925777458443955	0.7923609641096477
>10	1.8302710748737743	14.47535622028153
>50	0.27399739936381523	7.433363172585675
>100	0.23232959208729034	18.021178016355698
>500	0.02272789487810449	6.074943933968664
>1k	0.005681973719526123	3.4701150387873048
>5k	0.0012626608265613605	3.1343287376121203
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7400	1.7963781133174734	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5528	1.341943001407972	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2845	0.6906345584308394	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1747	0.4240908870223819	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1702	0.4131669660630189	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1549	0.3760256348011846	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1502	0.3646162062436277	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1433	0.3478661941059378	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1227	0.29785891149196486	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1221	0.2964023886973831	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	1087	0.263873379618391	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	957	0.23231538573578675	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	920	0.2233334951691994	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	885	0.21483711220080595	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	871	0.21143855901344855	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	859	0.2085255134242851	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	818	0.19857260766130988	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	818	0.19857260766130988	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	809	0.19638782346943728	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	793	0.192503762683886	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	777	0.1886197018983347	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	776	0.18837694809923777	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	762	0.18497839491188037	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	758	0.18400737971549255	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	729	0.17696751954168083	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	729	0.17696751954168083	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	727	0.17648201194348692	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	726	0.17623925814438995	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	726	0.17623925814438995	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	715	0.17356896635432342	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	700	0.1699276593678691	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	697	0.16919939797057823	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	681	0.16531533718502694	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	623	0.1512356168374035	No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA	620	0.15050735544011262	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	607	0.1473515560518522	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	599	0.14540952565907656	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	585	0.14201097247171918	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	566	0.13739865028887702	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	565	0.13715589648978008	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	559	0.13569937369519833	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	558	0.13545661989610136	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	518	0.12574646793222313	No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC	511	0.12404719133854444	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	508	0.12331892994125358	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	504	0.12234791474486577	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	501	0.1216196533475749	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	500	0.12137689954847793	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	499	0.12113414574938099	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	492	0.11943486915570228	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	487	0.1182211001602175	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	483	0.11725008496382969	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	483	0.11725008496382969	No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT	480	0.11652182356653883	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	480	0.11652182356653883	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	476	0.11555080837015098	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	475	0.11530805457105404	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	471	0.11433703937466622	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	458	0.11118123998640579	No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA	444	0.1077826867990484	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	440	0.10681167160266059	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	436	0.10584065640627276	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	434	0.10535514880807885	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	425	0.10317036461620624	No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC	425	0.10317036461620624	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	423	0.10268485701801233	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	415	0.10074282662523668	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0014565227945817353	0.0	0.0
2	2.4275379909695585E-4	0.0	0.0024275379909695586	0.0	0.0
3	2.4275379909695585E-4	0.0	0.0038840607855512937	0.0	0.0
4	2.4275379909695585E-4	0.0	0.004855075981939117	4.855075981939117E-4	0.0
5	2.4275379909695585E-4	0.0	0.006554352575617808	4.855075981939117E-4	0.0
6	4.855075981939117E-4	0.0	0.012137689954847793	4.855075981939117E-4	0.0
7	4.855075981939117E-4	0.0	0.029615963489828617	7.282613972908676E-4	0.0
8	4.855075981939117E-4	0.0	0.033257270476282956	7.282613972908676E-4	0.0
9	4.855075981939117E-4	0.0	0.04199640724377336	0.0014565227945817353	0.0
10	0.004855075981939117	0.0	0.04660872942661553	0.0014565227945817353	0.0
11	0.005826091178326941	0.0	0.07064135553721415	0.001699276593678691	0.0
12	0.005826091178326941	0.0	0.07840947710831675	0.0019420303927756468	0.0
13	0.005826091178326941	0.0	0.08326455309025586	0.0024275379909695586	0.0
14	0.005826091178326941	0.0	0.08981890566587367	0.0026702917900665144	0.0
15	0.005826091178326941	0.0	0.09977181142884886	0.0029130455891634706	0.0
16	0.005826091178326941	0.0	0.10753993299995145	0.003398553187357382	0.0
17	0.005826091178326941	0.0	0.11021022479001796	0.003398553187357382	0.0
18	0.0060688449774238965	0.0	0.1121522551827936	0.0041268145846482494	0.0
19	0.006554352575617808	0.0	0.11579356216924795	0.004612322182842162	0.0
20	0.006554352575617808	0.0	0.11943486915570228	0.005583337379229985	0.0
21	0.006554352575617808	0.0	0.1242899451376414	0.006797106374714764	0.0
22	0.006554352575617808	0.0	0.1259892217313201	0.007525367772005632	0.0
23	0.006554352575617808	0.0	0.12720299072680488	0.009224644365684324	0.0
24	0.006554352575617808	0.0	0.1276884983249988	0.010438413361169102	0.0
25	0.006554352575617808	0.0	0.1281740059231927	0.011409428557556925	0.0
26	0.006554352575617808	0.0	0.12841675972228966	0.012137689954847793	0.0
27	0.006554352575617808	0.0	0.12841675972228966	0.014807981744914309	0.0
28	0.006554352575617808	0.0	0.12841675972228966	0.03762683886002816	0.0
29	0.006554352575617808	0.0	0.12890226732048357	0.09880079623246103	0.0
30	0.006554352575617808	0.0	0.12890226732048357	0.18400737971549255	0.0
31	0.006554352575617808	0.0	0.12890226732048357	0.3350002427537991	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACATCG	25	0.005492302	29.599998	21
CACTACT	25	0.005492302	29.599998	4
GATATCA	40	5.931019E-5	27.750002	1
GGTATCA	1180	0.0	26.652542	1
ATAGTAC	35	8.8596955E-4	26.428572	3
ACCATCG	50	9.0726135E-6	25.899998	18
GTATCAA	3255	0.0	23.70046	1
CGGGCCG	55	1.8988185E-5	23.545454	17
TACAGTG	40	0.0019290951	23.125002	5
TCGCTGT	50	2.6980264E-4	22.199999	25
CTCGCTG	50	2.6980264E-4	22.199999	24
ACTCGCT	50	2.6980264E-4	22.199999	23
AACTCGC	50	2.6980264E-4	22.199999	22
TCCGCCA	60	3.7188194E-5	21.583332	12
AGCTTCT	60	3.7188194E-5	21.583332	37
ATACTAG	155	0.0	21.483871	6
AAATCCG	70	5.0888175E-6	21.142859	30
CATACTA	160	0.0	20.8125	5
ACTAGTG	125	1.8189894E-11	20.72	8
TCATACT	170	0.0	20.676472	4
>>END_MODULE