FastQCFastQC Report
Fri 10 Feb 2017
ERR1632859.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632859.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375213
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9540.25425558282895316No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT7670.2044172243499026No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC7350.19588873519840727No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC7040.18762676133289624No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6630.17669963460754293No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG6590.175633573463606No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA5920.15777704930266276No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA5760.15351280472691511No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC5460.1455173461473883No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT5020.13379067356408225No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT4580.12206400098077624No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC4540.12099793983683935No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG4460.11886581754896551No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA4330.11540111883117056No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC4320.11513460354518634No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA4290.11433505768723366No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC3830.10207535453195919No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3760.10020974753006959No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGC303.594899E-430.83333436
CGTAAAG303.594899E-430.83333412
AAAGCGC250.00549168429.615
GGTATCA2350.027.5531921
TAACTAG509.069794E-625.90000226
GTATCAA6600.023.2651521
CAATACC400.001928776623.1254
GACTATG400.001928776623.1257
TATTGTC1107.4578566E-1121.8636365
ACTTCTA1059.767973E-1021.14285721
GTTCTAG806.9344424E-720.81251
TACCTTC806.9344424E-720.81257
AGCTTCG450.003821078220.55555521
GCCACGG450.003821078220.55555533
GGCTAAG450.003821078220.5555551
TTCTAAC1101.7444108E-920.18181823
TATTAGA851.24074E-619.5882342
AACTAGA851.24074E-619.58823427
TCTAACA1052.2473614E-819.3809524
TTATTGT1254.0927262E-1019.244
Produced by FastQC (version 0.11.5)