FastQCFastQC Report
Fri 10 Feb 2017
ERR1632858.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632858.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381812
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10140.2655757283689355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7800.2042890218222581No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4110.10764459996018982No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT303.5950163E-430.8333346
GGTAACG405.9299018E-527.7525
GCGGGTA405.9299018E-527.7522
CGGGTAA451.3209517E-424.66666823
TGCGGGT451.3209517E-424.66666821
CTAAGCA400.001928838623.1254
GTATCAA6300.022.317461
TTCTGCG502.697524E-422.218
GTAACGT502.697524E-422.226
GGTATCA2400.020.81251
TTCGCCG450.003821198620.55555724
GTCAACC555.134451E-420.18181815
TCTGCGG609.221421E-418.519
CTAGAAC500.007026488418.53
GTCGCCC701.21649304E-418.537
ATAGGGG500.007026488418.53
GCGAACT500.007026488418.528
TTAGATA609.221421E-418.54
ATAATAC609.221421E-418.53
ATTACCC902.1445649E-618.53
Produced by FastQC (version 0.11.5)