##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632858.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	381812
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.354004064827716	33.0	33.0	33.0	27.0	33.0
2	31.001244067761096	33.0	33.0	33.0	27.0	33.0
3	31.435242999172367	33.0	33.0	33.0	27.0	33.0
4	31.591584339936933	33.0	33.0	33.0	27.0	33.0
5	31.69185096330131	33.0	33.0	33.0	27.0	33.0
6	34.48640692277875	37.0	33.0	37.0	27.0	37.0
7	34.66418551538453	37.0	37.0	37.0	27.0	37.0
8	34.83369040260652	37.0	37.0	37.0	27.0	37.0
9	34.931819848511836	37.0	37.0	37.0	27.0	37.0
10	34.97156453961635	37.0	37.0	37.0	27.0	37.0
11	34.98751217876861	37.0	37.0	37.0	27.0	37.0
12	34.94780415492441	37.0	37.0	37.0	27.0	37.0
13	34.98206970970006	37.0	37.0	37.0	27.0	37.0
14	34.93333892072538	37.0	37.0	37.0	27.0	37.0
15	34.97346599897332	37.0	37.0	37.0	27.0	37.0
16	34.9448995840885	37.0	37.0	37.0	27.0	37.0
17	34.936793500466194	37.0	37.0	37.0	27.0	37.0
18	34.93706588582863	37.0	37.0	37.0	27.0	37.0
19	34.92042156873016	37.0	37.0	37.0	27.0	37.0
20	34.92312446963427	37.0	37.0	37.0	27.0	37.0
21	34.93649492420354	37.0	37.0	37.0	27.0	37.0
22	34.94548364116371	37.0	37.0	37.0	27.0	37.0
23	34.92765025719464	37.0	37.0	37.0	27.0	37.0
24	34.92088514766429	37.0	37.0	37.0	27.0	37.0
25	34.93009648727646	37.0	37.0	37.0	27.0	37.0
26	34.866536410589504	37.0	37.0	37.0	27.0	37.0
27	34.794762867589284	37.0	37.0	37.0	27.0	37.0
28	34.764494044189284	37.0	37.0	37.0	27.0	37.0
29	34.79863388264381	37.0	37.0	37.0	27.0	37.0
30	34.763524980880646	37.0	37.0	37.0	27.0	37.0
31	34.76542382114758	37.0	37.0	37.0	27.0	37.0
32	34.78823085707102	37.0	37.0	37.0	27.0	37.0
33	34.756642012299245	37.0	37.0	37.0	27.0	37.0
34	34.67967219469268	37.0	37.0	37.0	27.0	37.0
35	34.68929473143851	37.0	37.0	37.0	27.0	37.0
36	34.668449393942566	37.0	37.0	37.0	27.0	37.0
37	34.70204184258221	37.0	37.0	37.0	27.0	37.0
38	34.55716949702995	37.0	37.0	37.0	27.0	37.0
39	34.61860549170796	37.0	37.0	37.0	27.0	37.0
40	34.66668412726682	37.0	37.0	37.0	27.0	37.0
41	34.689726881292366	37.0	37.0	37.0	27.0	37.0
42	34.596172985657866	37.0	37.0	37.0	27.0	37.0
43	34.3379595193446	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	6.0
16	26.0
17	29.0
18	16.0
19	26.0
20	48.0
21	104.0
22	381.0
23	790.0
24	1513.0
25	2641.0
26	4180.0
27	6125.0
28	8178.0
29	10922.0
30	13777.0
31	17045.0
32	21731.0
33	27736.0
34	38350.0
35	67952.0
36	160235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.055021843210795	20.229851340450274	13.237142887075315	25.477983929263615
2	16.408598996364702	22.939561878620893	37.087624275821604	23.564214849192798
3	18.37736896692613	26.339140728945136	29.774339203587108	25.509151100541626
4	13.336668307963082	17.95386211014845	38.135260285166524	30.57420929672195
5	14.844216525410411	36.373922244455386	34.69639508449184	14.085466145642359
6	30.402658900191714	39.00898871696018	17.259803253957447	13.328549128890657
7	27.112296103841683	31.693084554702317	23.219280693115984	17.97533864834002
8	24.832378238504813	35.685363477313444	20.449854902412707	19.03240338176904
9	26.088231904707026	15.324033817690383	19.854011922097786	38.7337223555048
10	15.163483599258273	27.922380648067634	33.53823347616105	23.375902276513045
11	34.61048893172556	23.263019496506136	23.778980231108505	18.3475113406598
12	23.191518338868345	25.16788367049752	30.29527620923386	21.34532178140027
13	28.59417723906006	21.576849339465497	25.806941636197923	24.022031785276525
14	22.441672865179722	20.657286832263	27.21575015976449	29.685290142792788
15	24.460205546185033	28.30188679245283	23.71036007249641	23.527547588865723
16	23.447665343153176	27.889903931777944	24.786544163095975	23.87588656197291
17	22.900013619268123	27.247441149047173	26.099755900809825	23.752789330874883
18	23.637811278849277	26.696646517134088	27.038699674185203	22.626842529831436
19	24.956523105612185	26.001801933936076	26.7249326893864	22.31674227106534
20	24.544278335934962	26.0735650005762	26.71288487527893	22.669271788209905
21	23.642787549893665	26.52064366756414	26.80560066210596	23.030968120436235
22	23.403926539763027	26.404094161524522	26.729385142426114	23.46259415628634
23	23.322472840036458	26.40671325154788	26.98265114768525	23.288162760730412
24	23.568405393230176	26.74064722952657	26.12175625700607	23.569191120237186
25	23.854410023781337	26.294092380543304	26.764219039736837	23.087278555938525
26	23.712455344515103	26.576430285061758	26.635883628592083	23.075230741831056
27	23.635977915832925	26.58402564612951	26.361402994143713	23.418593443893855
28	23.236042869265503	26.463285596052504	26.85536337254984	23.44530816213215
29	23.979078708893383	26.408546614564237	26.330497731868043	23.281876944674345
30	23.694121714351564	26.218138769865796	26.841744104428354	23.24599541135428
31	23.823504761505664	26.055755188417333	26.565953924968312	23.55478612510869
32	23.03699202748997	26.230186583973264	26.80193393607325	23.930887452463516
33	23.192827883880025	26.2817826574335	27.14765381915707	23.3777356395294
34	23.59721538348716	25.977182487716465	26.78281457890271	23.642787549893665
35	23.4738562433868	26.559930017914574	26.578787466082783	23.38742627261584
36	23.958911715713494	26.473500047143624	26.33809309293579	23.229495144207096
37	23.807790221365487	25.84884707657172	26.512000670487048	23.831362031575747
38	23.715074434538465	26.414046703613298	26.391784438414717	23.479094423433523
39	23.28475794370004	26.164185515384535	26.846720375472742	23.70433616544268
40	23.260924224487447	26.414570521617968	27.00622295789551	23.31828229599908
41	23.48014205944287	25.793060459074102	26.8637444606246	23.863053020858434
42	23.340806470199993	26.512524488491717	26.77260012781159	23.374068913496696
43	23.715860161545475	25.72024975642463	26.943102888332476	23.62078719369742
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	9.0
2	11.0
3	63.5
4	116.0
5	116.0
6	140.5
7	165.0
8	163.0
9	161.0
10	207.0
11	253.0
12	253.0
13	416.5
14	580.0
15	1014.0
16	1448.0
17	1642.0
18	1836.0
19	1836.0
20	1803.5
21	1771.0
22	2018.0
23	2265.0
24	3241.5
25	4218.0
26	4218.0
27	5496.0
28	6774.0
29	8400.0
30	10026.0
31	12040.0
32	14054.0
33	14054.0
34	16436.0
35	18818.0
36	20532.5
37	22247.0
38	24190.5
39	26134.0
40	26134.0
41	27139.5
42	28145.0
43	29121.5
44	30098.0
45	29964.0
46	29830.0
47	29830.0
48	29613.0
49	29396.0
50	29053.5
51	28711.0
52	27598.5
53	26486.0
54	26486.0
55	25360.0
56	24234.0
57	21901.0
58	19568.0
59	17932.0
60	16296.0
61	16296.0
62	14505.5
63	12715.0
64	10749.5
65	8784.0
66	7448.0
67	6112.0
68	6112.0
69	5130.5
70	4149.0
71	3442.0
72	2735.0
73	2155.0
74	1575.0
75	1575.0
76	1256.5
77	938.0
78	702.0
79	466.0
80	371.5
81	277.0
82	277.0
83	233.0
84	189.0
85	159.0
86	129.0
87	98.5
88	68.0
89	68.0
90	44.5
91	21.0
92	12.5
93	4.0
94	3.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	381812.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.49891897320312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.98751663816263	64.51498758376717
2	6.482982179203886	9.400183994296448
3	1.7679945943268538	3.8453309051749103
4	0.7903673903048313	2.292031255550879
5	0.46573810100335644	1.6882754423687913
6	0.3107533681888275	1.3517569956583053
7	0.19294441562044212	0.9791783088078921
8	0.13990234143261981	0.8114214812547921
9	0.12034667720582978	0.7852503599495716
>10	0.6717392902094504	9.041718884361472
>50	0.04647666956392393	2.340228811968893
>100	0.02251213681551488	2.4773783291431735
>500	3.630989808954013E-4	0.20532941204246896
>1k	3.630989808954013E-4	0.2669282356552096
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1014	0.2655757283689355	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	780	0.2042890218222581	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	411	0.10764459996018982	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.238180046724567E-4	0.0	0.0
2	0.0	0.0	5.238180046724567E-4	0.0	0.0
3	0.0	0.0	5.238180046724567E-4	0.0	0.0
4	0.0	0.0	5.238180046724567E-4	0.0	0.0
5	0.0	0.0	0.0010476360093449133	0.0	0.0
6	0.0	0.0	0.0015714540140173699	0.0	0.0
7	0.0	0.0	0.0028809990256985114	2.619090023362283E-4	0.0
8	0.0	0.0	0.0031429080280347397	2.619090023362283E-4	0.0
9	0.0	0.0	0.0036667260327071963	2.619090023362283E-4	0.0
10	0.0	0.0	0.00471436204205211	2.619090023362283E-4	0.0
11	0.0	0.0	0.006547725058405707	2.619090023362283E-4	0.0
12	0.0	0.0	0.007857270070086849	2.619090023362283E-4	0.0
13	0.0	0.0	0.008381088074759306	2.619090023362283E-4	0.0
14	0.0	0.0	0.009690633086440447	2.619090023362283E-4	0.0
15	2.619090023362283E-4	0.0	0.01073826909578536	5.238180046724567E-4	0.0
16	5.238180046724567E-4	0.0	0.011523996102794046	7.857270070086849E-4	0.0
17	5.238180046724567E-4	0.0	0.012047814107466501	7.857270070086849E-4	0.0
18	5.238180046724567E-4	0.0	0.012309723109802731	0.0010476360093449133	0.0
19	5.238180046724567E-4	0.0	0.012309723109802731	0.0015714540140173699	0.0
20	5.238180046724567E-4	0.0	0.013357359119147644	0.0015714540140173699	0.0
21	5.238180046724567E-4	0.0	0.0138811771238201	0.0015714540140173699	0.0
22	5.238180046724567E-4	0.0	0.014143086126156328	0.0020952720186898266	0.0
23	5.238180046724567E-4	0.0	0.014404995128492557	0.002357181021026055	0.0
24	5.238180046724567E-4	0.0	0.014404995128492557	0.002619090023362283	0.0
25	5.238180046724567E-4	0.0	0.014404995128492557	0.0028809990256985114	0.0
26	5.238180046724567E-4	0.0	0.014404995128492557	0.003404817030370968	0.0
27	5.238180046724567E-4	0.0	0.014666904130828785	0.00471436204205211	0.0
28	5.238180046724567E-4	0.0	0.014666904130828785	0.00942872408410422	0.0
29	5.238180046724567E-4	0.0	0.014666904130828785	0.02121462918923449	0.0
30	5.238180046724567E-4	0.0	0.014666904130828785	0.039024441348098016	0.0
31	5.238180046724567E-4	0.0	0.014928813133165013	0.0935015138340335	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGT	30	3.5950163E-4	30.833334	6
GGTAACG	40	5.9299018E-5	27.75	25
GCGGGTA	40	5.9299018E-5	27.75	22
CGGGTAA	45	1.3209517E-4	24.666668	23
TGCGGGT	45	1.3209517E-4	24.666668	21
CTAAGCA	40	0.0019288386	23.125	4
GTATCAA	630	0.0	22.31746	1
TTCTGCG	50	2.697524E-4	22.2	18
GTAACGT	50	2.697524E-4	22.2	26
GGTATCA	240	0.0	20.8125	1
TTCGCCG	45	0.0038211986	20.555557	24
GTCAACC	55	5.134451E-4	20.181818	15
TCTGCGG	60	9.221421E-4	18.5	19
CTAGAAC	50	0.0070264884	18.5	3
GTCGCCC	70	1.21649304E-4	18.5	37
ATAGGGG	50	0.0070264884	18.5	3
GCGAACT	50	0.0070264884	18.5	28
TTAGATA	60	9.221421E-4	18.5	4
ATAATAC	60	9.221421E-4	18.5	3
ATTACCC	90	2.1445649E-6	18.5	3
>>END_MODULE