FastQCFastQC Report
Fri 10 Feb 2017
ERR1632857.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632857.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515693
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22930.4446443911396897No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17330.33605265148062896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8880.17219547288793915No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6880.1334127087239889No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5700.11053087786725824No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGGA250.00549356829.620
GGTATCA4950.023.9191931
ACGCCAT551.9000223E-523.5454546
CCAATAC400.001929751123.1253
TGTTACG400.001929751123.12516
GTATCAA12700.023.0157491
CGTTATT502.699303E-422.210
TACGCCA450.003822991220.5555555
TTTACCG555.1378104E-420.18181830
CGCCGTT555.1378104E-420.18181825
TTCGCCG1101.7480488E-920.18181824
GAATTAG656.892704E-519.9230751
CGCCGTC1153.0395313E-919.30434826
CGCGGTA701.2175433E-418.522
ATAGGCG500.007029750418.53
CCCTAAA902.147537E-618.51
GTATAAC500.007029750418.51
GGACCGT902.147537E-618.56
CCGTTTA609.227405E-418.527
TCGCCGT953.5985886E-617.52631625
Produced by FastQC (version 0.11.5)