##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632857.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	515693
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.803057245299044	33.0	33.0	33.0	33.0	33.0
2	31.31290128041296	33.0	33.0	33.0	27.0	33.0
3	31.754828938922962	33.0	33.0	33.0	27.0	33.0
4	31.883227036240555	33.0	33.0	33.0	33.0	33.0
5	31.984285223960768	33.0	33.0	33.0	33.0	33.0
6	34.89190080144582	37.0	37.0	37.0	27.0	37.0
7	35.08280314062824	37.0	37.0	37.0	33.0	37.0
8	35.237542491365986	37.0	37.0	37.0	33.0	37.0
9	35.325877993302214	37.0	37.0	37.0	33.0	37.0
10	35.36627218131718	37.0	37.0	37.0	33.0	37.0
11	35.40017219547289	37.0	37.0	37.0	33.0	37.0
12	35.340324572953286	37.0	37.0	37.0	33.0	37.0
13	35.36698966245421	37.0	37.0	37.0	33.0	37.0
14	35.33612633873254	37.0	37.0	37.0	33.0	37.0
15	35.36325682140343	37.0	37.0	37.0	33.0	37.0
16	35.36141657924385	37.0	37.0	37.0	33.0	37.0
17	35.33086351763549	37.0	37.0	37.0	33.0	37.0
18	35.33431130536967	37.0	37.0	37.0	33.0	37.0
19	35.346169135512795	37.0	37.0	37.0	33.0	37.0
20	35.344162127467314	37.0	37.0	37.0	33.0	37.0
21	35.34650654556102	37.0	37.0	37.0	33.0	37.0
22	35.341681969699025	37.0	37.0	37.0	33.0	37.0
23	35.33340378868823	37.0	37.0	37.0	33.0	37.0
24	35.334633202312226	37.0	37.0	37.0	33.0	37.0
25	35.31945362841846	37.0	37.0	37.0	33.0	37.0
26	35.29607149990401	37.0	37.0	37.0	33.0	37.0
27	35.254880326085484	37.0	37.0	37.0	33.0	37.0
28	35.20011130653315	37.0	37.0	37.0	33.0	37.0
29	35.24634230055479	37.0	37.0	37.0	33.0	37.0
30	35.22168615823755	37.0	37.0	37.0	33.0	37.0
31	35.20363084238103	37.0	37.0	37.0	33.0	37.0
32	35.23131591857946	37.0	37.0	37.0	33.0	37.0
33	35.21042752180076	37.0	37.0	37.0	33.0	37.0
34	35.12509186667261	37.0	37.0	37.0	33.0	37.0
35	35.15423905308003	37.0	37.0	37.0	33.0	37.0
36	35.11753698421347	37.0	37.0	37.0	33.0	37.0
37	35.14139614072714	37.0	37.0	37.0	33.0	37.0
38	34.99483801408978	37.0	37.0	37.0	27.0	37.0
39	35.059198011219856	37.0	37.0	37.0	27.0	37.0
40	35.08867485112266	37.0	37.0	37.0	33.0	37.0
41	35.09720899837694	37.0	37.0	37.0	33.0	37.0
42	35.00877847866851	37.0	37.0	37.0	27.0	37.0
43	34.802603486958326	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	14.0
16	30.0
17	24.0
18	28.0
19	18.0
20	42.0
21	95.0
22	254.0
23	633.0
24	1247.0
25	2204.0
26	3755.0
27	5814.0
28	8286.0
29	11232.0
30	14994.0
31	19295.0
32	24811.0
33	32599.0
34	46890.0
35	86855.0
36	256569.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.674600973835204	19.800346330083983	13.571834405353572	24.953218290727236
2	16.05548262241295	23.142644945733217	37.89405712313334	22.907815308720497
3	18.386132834845537	26.362390026624364	30.253270841372675	24.998206297157417
4	13.06610716065566	17.696963115652142	38.679020269811694	30.557909453880505
5	14.685675392142224	36.3640770768655	34.3995361581406	14.550711372851676
6	29.851869232275792	39.19386146408813	17.38088358771595	13.57338571592013
7	26.597413577457907	32.221108295051515	22.98576866469004	18.19570946280054
8	24.83493086002719	35.451130808446116	20.705923873312223	19.008014458214483
9	26.501038408510492	15.037822890750894	19.677017140042622	38.78412156069599
10	15.132258921490111	27.793861851915775	33.53254746525549	23.541331761338625
11	34.67780249101694	23.236111407368337	23.843449494175797	18.24263660743892
12	23.174834639989296	24.975712293942326	30.58738435464511	21.262068711423268
13	29.092114882304003	21.795331718677584	25.94023963869977	23.172313760318637
14	22.47771445414229	20.68498118066369	27.68682142282327	29.150482942370754
15	24.261333778042363	28.02171059137898	24.220417961849392	23.496537668729264
16	22.98557475086922	27.8648343103358	25.235168986199152	23.914421952595827
17	22.76528865041798	27.019757879203326	26.226844265871364	23.98810920450733
18	23.95107166473076	26.089553280730975	27.213477786202255	22.74589726833601
19	25.077710963693516	26.172936223683468	26.80664659012242	21.942706222500597
20	24.892329350989833	25.616403557930784	26.788806518607	22.702460572472383
21	23.766077879668718	26.38546577130192	26.986986443484785	22.861469905544578
22	23.551221366200433	26.035839152363906	27.212702130918977	23.200237350516684
23	23.332098748674117	26.005976423957666	27.432406489907756	23.229518337460465
24	23.416063433089064	26.176426672458224	26.97476987277314	23.432740021679564
25	23.633246912407188	26.060660121428835	27.560389611648787	22.745703354515186
26	23.68696104077426	26.07287669214048	26.97476987277314	23.26539239431212
27	23.723416839088372	26.254380028427764	27.266998000748504	22.755205131735355
28	23.338497904761166	26.48281050935343	26.84988937216522	23.32880221372018
29	23.664273123738347	26.418818948482915	26.59469878396643	23.322209143812305
30	23.805054557653488	26.287927119429582	27.08995468234007	22.817063640576855
31	23.82463985355628	25.940821380162227	26.86036071848949	23.374178047792
32	22.93670846802264	26.131244752207223	27.158018433447808	23.774028346322325
33	23.181621623717987	26.222384247992508	27.369384498141336	23.22660963014817
34	23.093972576707458	26.00112857843717	27.04632407265563	23.858574772199738
35	23.120926597801407	26.379842270498145	27.170235004159448	23.328996127541
36	23.489750685000573	26.26000352923154	27.116520875792382	23.13372490997551
37	23.743777790274446	25.906304720056312	26.928230555776402	23.42168693389284
38	23.57487885234044	26.164597929388222	26.708138369145985	23.552384849125353
39	23.27237329186163	25.97708326465552	27.25032141215801	23.50022203132484
40	23.282456810544257	26.280170566596794	27.348829633134443	23.088542989724505
41	23.526206483314684	25.76610502760363	27.07735028398679	23.63033820509489
42	23.020479238616772	26.44674253868096	27.260211017019813	23.272567205682453
43	23.252012340675556	25.94954750209912	27.18574810982503	23.612692047400294
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	17.0
2	28.0
3	123.5
4	219.0
5	219.0
6	305.5
7	392.0
8	374.0
9	356.0
10	457.5
11	559.0
12	559.0
13	908.0
14	1257.0
15	2159.0
16	3061.0
17	3408.5
18	3756.0
19	3756.0
20	3381.5
21	3007.0
22	3169.0
23	3331.0
24	4559.0
25	5787.0
26	5787.0
27	7390.0
28	8993.0
29	11201.5
30	13410.0
31	15952.0
32	18494.0
33	18494.0
34	21254.0
35	24014.0
36	26408.0
37	28802.0
38	30971.0
39	33140.0
40	33140.0
41	35175.5
42	37211.0
43	38021.5
44	38832.0
45	39920.5
46	41009.0
47	41009.0
48	41418.5
49	41828.0
50	41048.5
51	40269.0
52	39029.0
53	37789.0
54	37789.0
55	35370.5
56	32952.0
57	29867.5
58	26783.0
59	24582.0
60	22381.0
61	22381.0
62	19193.0
63	16005.0
64	13270.5
65	10536.0
66	9263.0
67	7990.0
68	7990.0
69	6650.0
70	5310.0
71	4356.0
72	3402.0
73	2809.0
74	2216.0
75	2216.0
76	1651.0
77	1086.0
78	860.5
79	635.0
80	509.5
81	384.0
82	384.0
83	309.5
84	235.0
85	181.0
86	127.0
87	97.5
88	68.0
89	68.0
90	48.0
91	28.0
92	16.0
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	515693.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.61789885534566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.67316394746486	62.789677879176764
2	7.6866363012546906	11.010014823221734
3	1.997347289713038	4.291374485210015
4	0.873331067537447	2.5018454424851178
5	0.4589956640498825	1.6436152521483356
6	0.2771282784701755	1.190840701045986
7	0.1875298957687048	0.9401347975261631
8	0.13137542541939923	0.7527065543811634
9	0.0916438698479468	0.5907007261334444
>10	0.5498288488050462	7.14477746438468
>50	0.043321220558795726	2.199315680009884
>100	0.028063428296575123	3.642110092580527
>500	0.0010898418755950687	0.5172908575103436
>1k	5.449209377975344E-4	0.785595244185833
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2293	0.4446443911396897	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1733	0.33605265148062896	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	888	0.17219547288793915	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	688	0.1334127087239889	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	570	0.11053087786725824	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.939138208197513E-4	5.817414624592538E-4	0.0	0.0
2	0.0	1.939138208197513E-4	5.817414624592538E-4	0.0	0.0
3	0.0	1.939138208197513E-4	0.0011634829249185076	0.0	0.0
4	0.0	1.939138208197513E-4	0.0015513105665580103	0.0	0.0
5	1.939138208197513E-4	1.939138208197513E-4	0.002908707312296269	0.0	0.0
6	1.939138208197513E-4	1.939138208197513E-4	0.005041759341313534	0.0	0.0
7	1.939138208197513E-4	1.939138208197513E-4	0.010083518682627067	1.939138208197513E-4	0.0
8	1.939138208197513E-4	1.939138208197513E-4	0.01202265689082458	1.939138208197513E-4	0.0
9	1.939138208197513E-4	1.939138208197513E-4	0.014737450382301098	0.0011634829249185076	0.0
10	1.939138208197513E-4	1.939138208197513E-4	0.016094847128039356	0.0011634829249185076	0.0
11	3.878276416395026E-4	1.939138208197513E-4	0.023851399960829408	0.0011634829249185076	0.0
12	3.878276416395026E-4	1.939138208197513E-4	0.025402710527387417	0.0011634829249185076	0.0
13	3.878276416395026E-4	1.939138208197513E-4	0.02714793491476518	0.0011634829249185076	0.0
14	3.878276416395026E-4	1.939138208197513E-4	0.02850533166050344	0.0011634829249185076	0.0
15	3.878276416395026E-4	1.939138208197513E-4	0.03141403897279971	0.0011634829249185076	0.0
16	3.878276416395026E-4	1.939138208197513E-4	0.032965349539357716	0.0011634829249185076	0.0
17	3.878276416395026E-4	1.939138208197513E-4	0.03354709100181697	0.0017452243873777617	0.0
18	3.878276416395026E-4	1.939138208197513E-4	0.034322746285095976	0.0017452243873777617	0.0
19	3.878276416395026E-4	1.939138208197513E-4	0.035680143030834235	0.0017452243873777617	0.0
20	3.878276416395026E-4	1.939138208197513E-4	0.037231453597392244	0.0023269658498370153	0.0
21	3.878276416395026E-4	1.939138208197513E-4	0.03936450562640951	0.0023269658498370153	0.0
22	3.878276416395026E-4	1.939138208197513E-4	0.039752333268049014	0.002520879670656767	0.0
23	5.817414624592538E-4	1.939138208197513E-4	0.04014016090968851	0.002714793491476518	0.0
24	5.817414624592538E-4	1.939138208197513E-4	0.04014016090968851	0.003296534953935772	0.0
25	5.817414624592538E-4	1.939138208197513E-4	0.04052798855132802	0.0034904487747555234	0.0
26	5.817414624592538E-4	1.939138208197513E-4	0.04052798855132802	0.0034904487747555234	0.0
27	5.817414624592538E-4	1.939138208197513E-4	0.04052798855132802	0.0046539316996740306	0.0
28	5.817414624592538E-4	1.939138208197513E-4	0.04052798855132802	0.012604398353283834	0.0
29	7.756552832790052E-4	1.939138208197513E-4	0.04052798855132802	0.03160795279361946	0.0
30	7.756552832790052E-4	1.939138208197513E-4	0.04052798855132802	0.05914371535002414	0.0
31	7.756552832790052E-4	1.939138208197513E-4	0.04052798855132802	0.13845446806530243	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGGA	25	0.005493568	29.6	20
GGTATCA	495	0.0	23.919193	1
ACGCCAT	55	1.9000223E-5	23.545454	6
CCAATAC	40	0.0019297511	23.125	3
TGTTACG	40	0.0019297511	23.125	16
GTATCAA	1270	0.0	23.015749	1
CGTTATT	50	2.699303E-4	22.2	10
TACGCCA	45	0.0038229912	20.555555	5
TTTACCG	55	5.1378104E-4	20.181818	30
CGCCGTT	55	5.1378104E-4	20.181818	25
TTCGCCG	110	1.7480488E-9	20.181818	24
GAATTAG	65	6.892704E-5	19.923075	1
CGCCGTC	115	3.0395313E-9	19.304348	26
CGCGGTA	70	1.2175433E-4	18.5	22
ATAGGCG	50	0.0070297504	18.5	3
CCCTAAA	90	2.147537E-6	18.5	1
GTATAAC	50	0.0070297504	18.5	1
GGACCGT	90	2.147537E-6	18.5	6
CCGTTTA	60	9.227405E-4	18.5	27
TCGCCGT	95	3.5985886E-6	17.526316	25
>>END_MODULE