FastQCFastQC Report
Fri 10 Feb 2017
ERR1632856.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632856.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524686
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53531.0202292418703758No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39040.744064068795432No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18930.3607872136858997No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG16280.31028081557350495No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA16040.3057066512161559No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14570.27768989452739357No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11740.22375287314698694No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG11590.22089402042364384No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10320.19668906736600558No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC10300.19630788700289317No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT9650.1839195252017397No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9520.18144185284150902No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9430.17972654120750317No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9030.1721029339452549No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG8770.1671475892247935No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG8740.1665758186801249No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG8690.16562286777234383No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC8630.1644793266830066No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA8600.16390755613833796No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC8370.15952398196254522No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC7880.15018506306629106No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7840.14942270234006627No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC7760.1478979808876166No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT7740.1475168005245042No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7720.14713562016139176No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG7690.14656384961672314No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7450.1419896852593742No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7290.13894024235447486No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7160.1364625699942442No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7110.13550961908646314No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG7000.13341312708934486No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7000.13341312708934486No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT6980.13303194672623245No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG6960.13265076636312004No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6880.13112604491067037No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA6860.130744864547558No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA6860.130744864547558No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6610.12598011000865278No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6580.12540833946398416No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA6540.12464597873775933No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6520.12426479837464695No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6510.12407420819309073No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT6490.12369302782997832No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC6370.12140594565130383No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT6340.1208341751066352No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC6320.12045299474352279No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT6260.11930945365418555No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG6140.11702237147551107No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT6140.11702237147551107No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6090.11606942056773004No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC6070.11568824020461763No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT5980.11397292857061175No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5970.11378233838905555No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG5910.1126387972997183No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG5690.10844581330548175No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC5660.10787404276081314No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5610.1069210918530321No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT5460.104062239129689No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5450.10387164894813279No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG5430.10349046858502038No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5370.10234692749568314No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5340.10177515695101451No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA5280.10063161586167726No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAC250.00549365529.626
GGTATCA8450.027.3668651
TAGCGGG358.862943E-426.42857230
GCCGTCC1850.026.027
GCCGGCA2450.024.9183715
GGCGTCA751.3700628E-824.66666425
GCCGCTC2300.024.13043627
CGCTCTC2300.024.13043629
CGCCGTC2150.024.09302326
CCGCTCT2350.023.61702228
TCGCCGT2200.023.54545425
GTATCAA24300.023.5246931
GGCAGCT2600.023.4807718
CGCCGGC2600.023.4807714
AGTCGCC2300.023.32608823
GCAGCTT2550.023.21568719
CTTCCCT400.001929795723.12500235
TAGACTA400.001929795723.1250025
GCAGGGG400.001929795723.1250029
TCCGCCG2100.022.90476431
Produced by FastQC (version 0.11.5)