##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632856.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	524686
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.823069416755928	33.0	33.0	33.0	33.0	33.0
2	31.31746797131999	33.0	33.0	33.0	27.0	33.0
3	31.757182390991947	33.0	33.0	33.0	27.0	33.0
4	31.87540548061126	33.0	33.0	33.0	33.0	33.0
5	31.991493960197147	33.0	33.0	33.0	33.0	33.0
6	34.860981615671086	37.0	37.0	37.0	27.0	37.0
7	35.05332522689761	37.0	37.0	37.0	33.0	37.0
8	35.2409002717816	37.0	37.0	37.0	33.0	37.0
9	35.32102247820601	37.0	37.0	37.0	33.0	37.0
10	35.31961401676431	37.0	37.0	37.0	33.0	37.0
11	35.36198030822244	37.0	37.0	37.0	33.0	37.0
12	35.29239011523082	37.0	37.0	37.0	33.0	37.0
13	35.32998021673915	37.0	37.0	37.0	33.0	37.0
14	35.288124706967594	37.0	37.0	37.0	33.0	37.0
15	35.327384378466355	37.0	37.0	37.0	33.0	37.0
16	35.32977247344126	37.0	37.0	37.0	33.0	37.0
17	35.30776121337333	37.0	37.0	37.0	33.0	37.0
18	35.30724852578494	37.0	37.0	37.0	33.0	37.0
19	35.30509676263517	37.0	37.0	37.0	33.0	37.0
20	35.29970877820258	37.0	37.0	37.0	33.0	37.0
21	35.29929329160679	37.0	37.0	37.0	33.0	37.0
22	35.2955958420846	37.0	37.0	37.0	33.0	37.0
23	35.293017156928144	37.0	37.0	37.0	33.0	37.0
24	35.297259694369586	37.0	37.0	37.0	33.0	37.0
25	35.27053323320996	37.0	37.0	37.0	33.0	37.0
26	35.245628814186006	37.0	37.0	37.0	33.0	37.0
27	35.18143232333243	37.0	37.0	37.0	33.0	37.0
28	35.16018723579436	37.0	37.0	37.0	33.0	37.0
29	35.2054695570303	37.0	37.0	37.0	33.0	37.0
30	35.137729994701594	37.0	37.0	37.0	33.0	37.0
31	35.11506691621275	37.0	37.0	37.0	33.0	37.0
32	35.14721376213583	37.0	37.0	37.0	33.0	37.0
33	35.113713725923695	37.0	37.0	37.0	33.0	37.0
34	35.057360021041156	37.0	37.0	37.0	33.0	37.0
35	35.029190792207146	37.0	37.0	37.0	27.0	37.0
36	34.992910045246106	37.0	37.0	37.0	27.0	37.0
37	35.000156283948876	37.0	37.0	37.0	27.0	37.0
38	34.82518115596757	37.0	37.0	37.0	27.0	37.0
39	34.89903675722241	37.0	37.0	37.0	27.0	37.0
40	34.89186103688682	37.0	37.0	37.0	27.0	37.0
41	34.89791799285668	37.0	37.0	37.0	27.0	37.0
42	34.75664302077814	37.0	37.0	37.0	27.0	37.0
43	34.51442195903836	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	20.0
16	30.0
17	19.0
18	20.0
19	34.0
20	46.0
21	100.0
22	259.0
23	630.0
24	1357.0
25	2360.0
26	3887.0
27	6051.0
28	8686.0
29	12085.0
30	16009.0
31	20597.0
32	26554.0
33	34812.0
34	49602.0
35	89801.0
36	251725.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.42997526139443	19.672337359868568	13.536667645029599	23.361019733707398
2	17.047148199113373	23.44831003686014	37.94917341038259	21.555368353643896
3	18.15981367903851	28.61673458030136	29.69661854899883	23.526833191661296
4	11.883297820029505	18.558528338854096	40.756376194523966	28.801797646592437
5	13.890593612179474	36.85461399770529	34.4383116759357	14.816480714179528
6	26.615156493598075	42.132056124996666	17.084122694335278	14.168664687069981
7	26.411987360059157	32.336673743915405	22.868915884929272	18.38242301109616
8	24.10660852395528	34.31976458300774	20.373137457450742	21.20048943558624
9	27.33958977369322	13.83551304970973	19.641271160274908	39.183626016322144
10	16.760119385689727	26.489557563952538	32.54441704181168	24.205906008546062
11	34.738681802068285	24.31206473967287	22.643638290329836	18.30561516792901
12	23.954517559073427	26.6067705256096	28.03352862473937	21.405183290577604
13	29.4086367846674	22.656598422675657	25.18611131228964	22.748653480367306
14	23.16051886271027	21.052782044880175	28.412040725309996	27.374658367099556
15	26.05424958927816	26.869975566338724	25.810103566704655	21.265671277678457
16	22.089973812909054	27.583354615903605	25.68031165306488	24.646359918122457
17	22.395108693580543	25.885577278600913	25.37098378839916	26.348330239419386
18	23.881712109718954	24.99151873692075	28.14635801222064	22.980411141139655
19	26.79278654280846	24.28595388479967	27.86009155952322	21.06116801286865
20	25.83564265103319	23.932599688194465	28.192099655794134	22.039658004978214
21	23.238279656785203	26.341850173246474	28.14254620858952	22.277323961378805
22	23.17100132269586	25.84364743865855	27.70628528300736	23.279065955638227
23	22.97507461605608	26.033284669306976	29.291423823010334	21.70021689162661
24	23.233514902246295	26.45582310181709	26.50042120430124	23.81024079163538
25	23.45879249684573	26.409700277880482	29.054329637154414	21.07717758811937
26	23.47213380955467	26.435239362209018	28.00265301532726	22.089973812909054
27	23.405808426373106	26.10647129902456	27.812062833771055	22.67565744083128
28	22.46048112585432	25.887673770598035	28.90300103299878	22.748844070548863
29	24.87544931635302	25.700514212309837	26.80746198678829	22.616574484548853
30	24.15425606934433	25.46189530500147	28.45244584379991	21.93140278185429
31	23.97567306922617	25.177153573756495	27.97940101317741	22.867772343839935
32	21.357916925551663	25.273592205623935	28.886229097021836	24.482261771802563
33	21.558227206367235	25.658774962549032	29.526802697232252	23.256195133851485
34	22.408831186652588	25.236808300583586	28.962465169644318	23.391895343119504
35	22.70119652515981	26.62411423213122	28.063070102880577	22.61161913982839
36	23.738388293188688	26.063969688537526	27.630811571111103	22.566830447162683
37	23.494051680433632	25.443789237753627	27.716195972448283	23.345963109364458
38	23.04178117960075	26.078835722698912	27.189595300808485	23.689787796891856
39	22.2925711759033	25.506112227122507	28.56851526436764	23.632801332606547
40	22.510034573058935	27.023972433036143	28.65999855151462	21.805994442390304
41	23.264199921476845	24.81922521279394	28.109383516998736	23.807191348730477
42	21.83153352671884	27.152811395768133	28.064976004696142	22.950679072816886
43	23.52873909347686	25.270733352900592	27.756601090938197	23.44392646268435
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27.0
1	42.0
2	57.0
3	281.0
4	505.0
5	505.0
6	645.0
7	785.0
8	783.0
9	781.0
10	1020.5
11	1260.0
12	1260.0
13	1952.0
14	2644.0
15	4571.5
16	6499.0
17	7000.5
18	7502.0
19	7502.0
20	5564.0
21	3626.0
22	2655.0
23	1684.0
24	1811.0
25	1938.0
26	1938.0
27	2573.0
28	3208.0
29	4686.0
30	6164.0
31	8909.0
32	11654.0
33	11654.0
34	16163.0
35	20672.0
36	23294.5
37	25917.0
38	29677.5
39	33438.0
40	33438.0
41	37492.0
42	41546.0
43	44657.0
44	47768.0
45	48975.5
46	50183.0
47	50183.0
48	50838.0
49	51493.0
50	50906.0
51	50319.0
52	47713.0
53	45107.0
54	45107.0
55	41668.0
56	38229.0
57	31748.5
58	25268.0
59	22285.0
60	19302.0
61	19302.0
62	15901.5
63	12501.0
64	8974.0
65	5447.0
66	4848.5
67	4250.0
68	4250.0
69	3366.0
70	2482.0
71	1838.0
72	1194.0
73	1066.0
74	938.0
75	938.0
76	557.5
77	177.0
78	115.5
79	54.0
80	42.5
81	31.0
82	31.0
83	25.0
84	19.0
85	15.0
86	11.0
87	7.0
88	3.0
89	3.0
90	2.5
91	2.0
92	1.0
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	524686.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.289515409974143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.84497441308172	13.66565225660364
2	9.792943204205496	3.7780225769304696
3	4.207403899853864	2.4347634708664923
4	2.422585482143154	1.8692199995912004
5	1.6549764021059108	1.5961846405782767
6	1.2792053504842993	1.480515079241296
7	0.9693975992992392	1.308944695105223
8	0.8104963298943776	1.2507265156180067
9	0.6705832757750019	1.1641703788559556
>10	5.741432069905495	23.22827382256668
>50	0.8374795604136395	11.38820419330023
>100	0.703562781063487	25.608471150947025
>500	0.05496584227058492	7.326234653612897
>1k	0.008994410553368441	2.8686900821075025
>5k	9.993789503742715E-4	1.031926484075093
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5353	1.0202292418703758	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3904	0.744064068795432	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1893	0.3607872136858997	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1628	0.31028081557350495	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1604	0.3057066512161559	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1457	0.27768989452739357	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1174	0.22375287314698694	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	1159	0.22089402042364384	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1032	0.19668906736600558	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1030	0.19630788700289317	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	965	0.1839195252017397	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	952	0.18144185284150902	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	943	0.17972654120750317	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	903	0.1721029339452549	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	877	0.1671475892247935	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	874	0.1665758186801249	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	869	0.16562286777234383	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	863	0.1644793266830066	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	860	0.16390755613833796	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	837	0.15952398196254522	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	788	0.15018506306629106	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	784	0.14942270234006627	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	776	0.1478979808876166	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	774	0.1475168005245042	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	772	0.14713562016139176	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	769	0.14656384961672314	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	745	0.1419896852593742	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	729	0.13894024235447486	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	716	0.1364625699942442	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	711	0.13550961908646314	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	700	0.13341312708934486	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	700	0.13341312708934486	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	698	0.13303194672623245	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	696	0.13265076636312004	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	688	0.13112604491067037	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	686	0.130744864547558	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	686	0.130744864547558	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	661	0.12598011000865278	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	658	0.12540833946398416	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	654	0.12464597873775933	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	652	0.12426479837464695	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	651	0.12407420819309073	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	649	0.12369302782997832	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	637	0.12140594565130383	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	634	0.1208341751066352	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	632	0.12045299474352279	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	626	0.11930945365418555	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	614	0.11702237147551107	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	614	0.11702237147551107	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	609	0.11606942056773004	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	607	0.11568824020461763	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	598	0.11397292857061175	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	597	0.11378233838905555	No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG	591	0.1126387972997183	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	569	0.10844581330548175	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	566	0.10787404276081314	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	561	0.1069210918530321	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT	546	0.104062239129689	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	545	0.10387164894813279	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	543	0.10349046858502038	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	537	0.10234692749568314	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	534	0.10177515695101451	No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA	528	0.10063161586167726	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	9.529509077810347E-4	0.0	0.0
2	0.0	0.0	0.0011435410893372417	0.0	0.0
3	0.0	0.0	0.0013341312708934486	0.0	0.0
4	0.0	0.0	0.0019059018155620695	7.623607262248278E-4	0.0
5	1.9059018155620695E-4	0.0	0.0022870821786744834	7.623607262248278E-4	0.0
6	1.9059018155620695E-4	0.0	0.006098885809798622	9.529509077810347E-4	0.0
7	1.9059018155620695E-4	0.0	0.014484853798271729	9.529509077810347E-4	0.0
8	3.811803631124139E-4	0.0	0.017915477066283454	9.529509077810347E-4	0.0
9	3.811803631124139E-4	0.0	0.02248964142363242	0.0020964919971182766	0.0
10	0.0020964919971182766	0.0	0.026682625417868974	0.0020964919971182766	0.0
11	0.0022870821786744834	0.0	0.040405118489915876	0.0020964919971182766	0.0
12	0.0026682625417868973	0.0	0.04478869266570863	0.0022870821786744834	0.0
13	0.0028588527233431044	0.0	0.04840990611527657	0.0022870821786744834	0.0
14	0.0028588527233431044	0.0	0.05184052938328829	0.0024776723602306905	0.0
15	0.0028588527233431044	0.0	0.05679587410374967	0.0024776723602306905	0.0
16	0.0028588527233431044	0.0	0.06232298936887967	0.0026682625417868973	0.0
17	0.0028588527233431044	0.0	0.06327594027666071	0.0028588527233431044	0.0
18	0.0028588527233431044	0.0	0.06518184209222277	0.003049442904899311	0.0
19	0.0028588527233431044	0.0	0.06594420281844761	0.0032400330864555183	0.0
20	0.0028588527233431044	0.0	0.06804069481556588	0.0036212134495679322	0.0
21	0.0028588527233431044	0.0	0.07013718681268416	0.005145934902017588	0.0
22	0.0028588527233431044	0.0	0.07185249844669002	0.006098885809798622	0.0
23	0.0028588527233431044	0.0	0.07223367880980243	0.007623607262248278	0.0
24	0.0028588527233431044	0.0	0.07242426899135865	0.009148328714697933	0.0
25	0.0028588527233431044	0.0	0.07280544935447106	0.009720099259366554	0.0
26	0.0028588527233431044	0.0	0.07299603953602726	0.010291869804035176	0.0
27	0.0028588527233431044	0.0	0.07299603953602726	0.01315072252737828	0.0
28	0.0028588527233431044	0.0	0.07318662971758347	0.03316269159078001	0.0
29	0.0028588527233431044	0.0	0.07318662971758347	0.07147131808357761	0.0
30	0.0028588527233431044	0.0	0.07318662971758347	0.12655188055332142	0.0
31	0.0028588527233431044	0.0	0.07318662971758347	0.257868515645548	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTCAC	25	0.005493655	29.6	26
GGTATCA	845	0.0	27.366865	1
TAGCGGG	35	8.862943E-4	26.428572	30
GCCGTCC	185	0.0	26.0	27
GCCGGCA	245	0.0	24.91837	15
GGCGTCA	75	1.3700628E-8	24.666664	25
GCCGCTC	230	0.0	24.130436	27
CGCTCTC	230	0.0	24.130436	29
CGCCGTC	215	0.0	24.093023	26
CCGCTCT	235	0.0	23.617022	28
TCGCCGT	220	0.0	23.545454	25
GTATCAA	2430	0.0	23.524693	1
GGCAGCT	260	0.0	23.48077	18
CGCCGGC	260	0.0	23.48077	14
AGTCGCC	230	0.0	23.326088	23
GCAGCTT	255	0.0	23.215687	19
CTTCCCT	40	0.0019297957	23.125002	35
TAGACTA	40	0.0019297957	23.125002	5
GCAGGGG	40	0.0019297957	23.125002	9
TCCGCCG	210	0.0	22.904764	31
>>END_MODULE