FastQCFastQC Report
Fri 10 Feb 2017
ERR1632855.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632855.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428731
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19950.4653267433425621No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14250.33237624524468723No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7620.17773382377294855No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC6760.1576746258143218No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT6720.15674163986275777No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC6480.15114372415337354No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA6460.15067723117759155No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG5640.13155101917052883No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA5460.12735258238849068No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT5350.1247868710216896No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC5000.1166232439455043No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT4690.10939260282088303No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4690.10939260282088303No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC4640.10822637038142799No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG4570.10659364496619092No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4500.10496091955095387No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA4480.10449442657517186No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC4320.10076248276891572No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG200.001840648937.03
CGGGACG250.005492547529.617
GGTATCA3650.028.3835621
CGAGTCG509.073738E-625.90000221
CCGTCCG451.3213225E-424.66666628
GTATCAA10500.023.7857131
GCGAGTC551.8990528E-523.54545420
GTCGCCC400.00192922323.12500237
GTACTAT502.6982743E-422.21
CGTCCGC502.6982743E-422.229
GCCGCTC855.1615643E-821.76470627
CGCCGTC603.7192738E-521.58333426
GGCGAGT603.7192738E-521.58333419
CGATCTG603.7192738E-521.58333410
TATCACT1650.021.303034
GAGTCGC705.0896197E-621.14285722
GTCCGCC555.135866E-420.18181830
TCGCCGT656.8892376E-519.92307725
CTAAGAG656.8892376E-519.9230773
GTCCGGC656.8892376E-519.92307715