##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632855.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	428731
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80155388810233	33.0	33.0	33.0	33.0	33.0
2	31.332588499548667	33.0	33.0	33.0	27.0	33.0
3	31.765678245799815	33.0	33.0	33.0	27.0	33.0
4	31.870049051736405	33.0	33.0	33.0	33.0	33.0
5	31.991250924239207	33.0	33.0	33.0	33.0	33.0
6	34.89126748473985	37.0	37.0	37.0	27.0	37.0
7	35.0223520109346	37.0	37.0	37.0	33.0	37.0
8	35.224163869652536	37.0	37.0	37.0	33.0	37.0
9	35.282827693822	37.0	37.0	37.0	33.0	37.0
10	35.34908369117232	37.0	37.0	37.0	33.0	37.0
11	35.37937541255472	37.0	37.0	37.0	33.0	37.0
12	35.29458098434683	37.0	37.0	37.0	33.0	37.0
13	35.34375634138889	37.0	37.0	37.0	33.0	37.0
14	35.299964313287354	37.0	37.0	37.0	33.0	37.0
15	35.35556794353569	37.0	37.0	37.0	33.0	37.0
16	35.332744774695556	37.0	37.0	37.0	33.0	37.0
17	35.333757064453	37.0	37.0	37.0	33.0	37.0
18	35.28733634843293	37.0	37.0	37.0	33.0	37.0
19	35.320380378372455	37.0	37.0	37.0	33.0	37.0
20	35.21771227179747	37.0	37.0	37.0	33.0	37.0
21	35.286928167079125	37.0	37.0	37.0	33.0	37.0
22	35.320137802025044	37.0	37.0	37.0	33.0	37.0
23	35.30400414245762	37.0	37.0	37.0	33.0	37.0
24	35.29685280513889	37.0	37.0	37.0	33.0	37.0
25	35.29195462889317	37.0	37.0	37.0	33.0	37.0
26	35.23390424298686	37.0	37.0	37.0	33.0	37.0
27	35.198581394860646	37.0	37.0	37.0	33.0	37.0
28	35.1930347933786	37.0	37.0	37.0	33.0	37.0
29	35.23511945718877	37.0	37.0	37.0	33.0	37.0
30	35.199192500658924	37.0	37.0	37.0	33.0	37.0
31	35.18112756017176	37.0	37.0	37.0	33.0	37.0
32	35.21489232175887	37.0	37.0	37.0	33.0	37.0
33	35.19019851608584	37.0	37.0	37.0	33.0	37.0
34	35.13384849707626	37.0	37.0	37.0	33.0	37.0
35	35.13594071807264	37.0	37.0	37.0	33.0	37.0
36	35.11871779740677	37.0	37.0	37.0	33.0	37.0
37	35.12540264174972	37.0	37.0	37.0	33.0	37.0
38	34.96515997210372	37.0	37.0	37.0	27.0	37.0
39	35.021199773284415	37.0	37.0	37.0	27.0	37.0
40	35.068490498704314	37.0	37.0	37.0	27.0	37.0
41	35.08891822611381	37.0	37.0	37.0	33.0	37.0
42	34.951580361578706	37.0	37.0	37.0	27.0	37.0
43	34.75558100533901	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	13.0
16	22.0
17	18.0
18	14.0
19	19.0
20	33.0
21	81.0
22	195.0
23	550.0
24	1146.0
25	2009.0
26	3219.0
27	4877.0
28	7010.0
29	9335.0
30	12366.0
31	16298.0
32	20881.0
33	27551.0
34	39531.0
35	73640.0
36	209922.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.46679386375139	20.239031000790707	14.184885161091687	24.10928997436621
2	15.450947097364082	23.887472564381863	39.14319235138117	21.518387986872888
3	17.830994259803933	27.31666242935547	31.473114843573242	23.379228467267353
4	12.738523689679543	18.35929755487707	39.329323048718194	29.5728557067252
5	13.455756639944394	37.566679339725844	35.37439560003825	13.603168420291512
6	28.44137699396591	40.01623395555721	17.975607082296357	13.566781968180514
7	25.322638204375238	33.15738773263421	23.655858801906092	17.864115261084457
8	23.429143215676028	36.6339266346497	21.033701785035372	18.9032283646389
9	25.55915014309672	16.03313033114004	20.228301662347718	38.17941786341552
10	14.609160522565432	28.79637814853603	33.951592023903096	22.64286930499544
11	32.92460773771899	24.527967420130572	24.479918643625023	18.067506198525415
12	22.294865545062056	26.12640560164765	30.62922905038357	20.94949980290672
13	27.811378230172302	22.675757059788072	27.169017402520463	22.343847307519166
14	22.086109938399602	22.0005084773436	28.014302674637477	27.89907890961932
15	23.759420242529696	28.718940314556214	25.444859364030126	22.076780078883964
16	22.391896084024715	29.256806715632877	25.24520037039542	23.106096829946985
17	22.16634673023411	27.73067494536201	26.474875854556817	23.62810246984706
18	22.494291292208867	27.25858405387061	28.04928964782113	22.197835006099396
19	24.8048310012572	26.501465954176396	28.372569280038068	20.32113376452834
20	24.678877897796053	25.682770781678954	28.461202945436646	21.177148375088343
21	22.58269171111956	27.679827211001772	27.29357102705426	22.44391005082441
22	22.112700038019177	27.532415430654655	27.4325859338373	22.92229859748887
23	22.133458975441478	27.53428140255778	28.1227622915068	22.209497330493946
24	23.217122158183106	27.31176425310976	27.359813029615303	22.11130055909183
25	23.021894847818327	27.05752558130856	28.063051190606696	21.857528380266412
26	22.841828559166473	27.117469928696547	27.451245652868582	22.589455859268398
27	22.747596978058503	26.84340530542461	27.68215967588068	22.726838040636203
28	21.817409984349162	27.47433705516979	27.98561335662688	22.722639603854162
29	22.858389059806733	27.684725387247482	27.102775399959416	22.35411015298637
30	22.464902234734602	27.687757591590064	27.71458093769753	22.132759235977804
31	22.985974888683113	26.911746526376678	27.845432217404387	22.25684636753582
32	21.767028742964705	27.160154035980604	28.054654317042623	23.018162904012073
33	22.17124490647982	26.828477530199592	28.370470061647048	22.629807501673543
34	21.92097142497277	27.041431573644083	27.939897045000244	23.097699956382904
35	22.464668988246707	27.597491200776243	27.58746160179693	22.350378209180118
36	22.43411369833299	27.424189060273225	27.682626168856462	22.459071072537327
37	23.34330850813214	26.727015307967	27.329490986189477	22.600185197711387
38	22.942124548959605	27.217299425513904	27.374740804840332	22.465835220686163
39	22.5064201095792	26.91874392101341	27.90607630425605	22.66875966515134
40	22.50828608148233	27.043997285010878	28.067482873876624	22.380233759630165
41	22.217660957570132	26.910580293937226	28.3198555737747	22.551903174717946
42	22.59178832414731	26.903582899300492	27.676561760171296	22.828067016380903
43	22.321222398193736	26.70019196185953	27.870389591608724	23.108196048338
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8.0
1	22.0
2	36.0
3	121.5
4	207.0
5	207.0
6	258.5
7	310.0
8	300.5
9	291.0
10	409.0
11	527.0
12	527.0
13	832.0
14	1137.0
15	2033.5
16	2930.0
17	3321.0
18	3712.0
19	3712.0
20	3477.0
21	3242.0
22	3761.0
23	4280.0
24	5517.0
25	6754.0
26	6754.0
27	8325.5
28	9897.0
29	13129.0
30	16361.0
31	17639.5
32	18918.0
33	18918.0
34	20522.5
35	22127.0
36	23779.5
37	25432.0
38	27469.5
39	29507.0
40	29507.0
41	31504.5
42	33502.0
43	32103.5
44	30705.0
45	31450.0
46	32195.0
47	32195.0
48	32550.0
49	32905.0
50	33091.0
51	33277.0
52	33649.5
53	34022.0
54	34022.0
55	29178.0
56	24334.0
57	21632.0
58	18930.0
59	16547.0
60	14164.0
61	14164.0
62	12246.0
63	10328.0
64	8367.5
65	6407.0
66	5464.5
67	4522.0
68	4522.0
69	3725.5
70	2929.0
71	2501.0
72	2073.0
73	1691.5
74	1310.0
75	1310.0
76	952.0
77	594.0
78	470.5
79	347.0
80	269.5
81	192.0
82	192.0
83	162.5
84	133.0
85	120.5
86	108.0
87	82.0
88	56.0
89	56.0
90	37.5
91	19.0
92	11.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	428731.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.54802633524132
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.97701431920585	52.679766039272494
2	5.438974785314237	6.368824779345829
3	1.3970581383541802	2.4538499002867127
4	0.669084095090476	1.5669421287939318
5	0.40291959690817075	1.1795073585382196
6	0.2900820400582437	1.0190238552426345
7	0.1962156208349598	0.8041626135221667
8	0.15690263173627791	0.7349071531971415
9	0.1198815118347382	0.6316943320807916
>10	1.0896937642737505	13.211729658700111
>50	0.14672094752107281	6.190123288910502
>100	0.11144377981014138	11.171747873930187
>500	0.0032070152463350033	1.1850281521770103
>1k	8.017538115837508E-4	0.8026928660022529
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1995	0.4653267433425621	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1425	0.33237624524468723	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	762	0.17773382377294855	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	676	0.1576746258143218	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	672	0.15674163986275777	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	648	0.15114372415337354	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	646	0.15067723117759155	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	564	0.13155101917052883	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	546	0.12735258238849068	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	535	0.1247868710216896	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	500	0.1166232439455043	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	469	0.10939260282088303	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	469	0.10939260282088303	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	464	0.10822637038142799	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	457	0.10659364496619092	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	450	0.10496091955095387	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	448	0.10449442657517186	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	432	0.10076248276891572	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	4.6649297578201714E-4	0.0	0.0
4	0.0	0.0	4.6649297578201714E-4	0.0	0.0
5	0.0	0.0	6.997394636730258E-4	0.0	0.0
6	0.0	0.0	0.0030322043425831118	2.3324648789100857E-4	0.0
7	0.0	0.0	0.007463887612512274	2.3324648789100857E-4	0.0
8	0.0	0.0	0.008630120051967317	2.3324648789100857E-4	0.0
9	0.0	0.0	0.010729338442986396	0.00163272541523706	0.0
10	4.6649297578201714E-4	0.0	0.012128817370332447	0.0018659719031280685	0.0
11	4.6649297578201714E-4	0.0	0.01795997956760766	0.0018659719031280685	0.0
12	4.6649297578201714E-4	0.0	0.018892965519171696	0.0018659719031280685	0.0
13	4.6649297578201714E-4	0.0	0.020758937422299763	0.0018659719031280685	0.0
14	4.6649297578201714E-4	0.0	0.022158416349645816	0.0018659719031280685	0.0
15	4.6649297578201714E-4	0.0	0.023557895276991866	0.0018659719031280685	0.0
16	4.6649297578201714E-4	0.0	0.026823346107465985	0.0018659719031280685	0.0
17	4.6649297578201714E-4	0.0	0.02798957854692103	0.0018659719031280685	0.0
18	4.6649297578201714E-4	0.0	0.028922564498485064	0.0018659719031280685	0.0
19	4.6649297578201714E-4	0.0	0.029155810986376072	0.0018659719031280685	0.0
20	4.6649297578201714E-4	0.0	0.029855550450049097	0.0018659719031280685	0.0
21	4.6649297578201714E-4	2.3324648789100857E-4	0.031255029377395147	0.0018659719031280685	0.0
22	4.6649297578201714E-4	2.3324648789100857E-4	0.03172152235317717	0.002332464878910086	0.0
23	4.6649297578201714E-4	2.3324648789100857E-4	0.03218801532895919	0.002798957854692103	0.0
24	4.6649297578201714E-4	2.3324648789100857E-4	0.03218801532895919	0.0030322043425831118	0.0
25	4.6649297578201714E-4	2.3324648789100857E-4	0.03218801532895919	0.0030322043425831118	0.0
26	4.6649297578201714E-4	2.3324648789100857E-4	0.03218801532895919	0.003965190294147146	0.0
27	4.6649297578201714E-4	2.3324648789100857E-4	0.03242126181685019	0.004431683269929163	0.0
28	4.6649297578201714E-4	2.3324648789100857E-4	0.03242126181685019	0.010962584930877404	0.0
29	4.6649297578201714E-4	2.3324648789100857E-4	0.03242126181685019	0.027289839083248005	0.0
30	4.6649297578201714E-4	2.3324648789100857E-4	0.03242126181685019	0.048981762457111805	0.0
31	4.6649297578201714E-4	2.3324648789100857E-4	0.0326545083047412	0.12012194126386942	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGTGG	20	0.0018406489	37.0	3
CGGGACG	25	0.0054925475	29.6	17
GGTATCA	365	0.0	28.383562	1
CGAGTCG	50	9.073738E-6	25.900002	21
CCGTCCG	45	1.3213225E-4	24.666666	28
GTATCAA	1050	0.0	23.785713	1
GCGAGTC	55	1.8990528E-5	23.545454	20
GTCGCCC	40	0.001929223	23.125002	37
GTACTAT	50	2.6982743E-4	22.2	1
CGTCCGC	50	2.6982743E-4	22.2	29
GCCGCTC	85	5.1615643E-8	21.764706	27
CGCCGTC	60	3.7192738E-5	21.583334	26
GGCGAGT	60	3.7192738E-5	21.583334	19
CGATCTG	60	3.7192738E-5	21.583334	10
TATCACT	165	0.0	21.30303	4
GAGTCGC	70	5.0896197E-6	21.142857	22
GTCCGCC	55	5.135866E-4	20.181818	30
TCGCCGT	65	6.8892376E-5	19.923077	25
CTAAGAG	65	6.8892376E-5	19.923077	3
GTCCGGC	65	6.8892376E-5	19.923077	15
>>END_MODULE