FastQCFastQC Report
Fri 10 Feb 2017
ERR1632854.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632854.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372773
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12040.32298476552754624No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8450.22667950736775466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5150.13815378259691555No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC4180.11213258470972952No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC3920.10515783063687553No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAC200.00184034137.024
GCGCATA200.00184034137.023
GGTATCA2100.023.7857131
GTATCAA5950.022.3865551
CGAACTA909.43528E-820.55555529
GCGAACT909.43528E-820.55555528
GTCTAGA450.003821032820.5555551
ACGCTTC450.003821032820.55555525
CGCGCGC555.1341375E-420.18181810
TAGATCT555.1341375E-420.1818184
ATCGCGC555.1341375E-420.1818188
TCGCGCG555.1341375E-420.1818189
CGTGACG759.238256E-619.73333436
GTGACGT759.238256E-619.73333437
CGAACCG609.220861E-418.531
GCGAACC500.00702618518.530
GGCCGTT500.00702618518.514
ACACACG500.00702618518.56
GCGCCTT500.00702618518.513
CGCCTTT500.00702618518.514
Produced by FastQC (version 0.11.5)