##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632854.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	372773
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.757729234681698	33.0	33.0	33.0	27.0	33.0
2	31.293779324146332	33.0	33.0	33.0	27.0	33.0
3	31.723501970368027	33.0	33.0	33.0	27.0	33.0
4	31.849015352506754	33.0	33.0	33.0	27.0	33.0
5	31.951702510643205	33.0	33.0	33.0	33.0	33.0
6	34.864037899740595	37.0	37.0	37.0	27.0	37.0
7	34.96772566682673	37.0	37.0	37.0	27.0	37.0
8	35.18339847574798	37.0	37.0	37.0	33.0	37.0
9	35.21158721259319	37.0	37.0	37.0	33.0	37.0
10	35.28041462230365	37.0	37.0	37.0	33.0	37.0
11	35.32482502756369	37.0	37.0	37.0	33.0	37.0
12	35.23463877480397	37.0	37.0	37.0	33.0	37.0
13	35.287794448632276	37.0	37.0	37.0	33.0	37.0
14	35.22010714295295	37.0	37.0	37.0	33.0	37.0
15	35.29978297784442	37.0	37.0	37.0	33.0	37.0
16	35.25163303136225	37.0	37.0	37.0	33.0	37.0
17	35.261065044946925	37.0	37.0	37.0	33.0	37.0
18	35.2487894777787	37.0	37.0	37.0	33.0	37.0
19	35.29688845490419	37.0	37.0	37.0	33.0	37.0
20	35.16831422876657	37.0	37.0	37.0	33.0	37.0
21	35.2603890303214	37.0	37.0	37.0	33.0	37.0
22	35.28607758609127	37.0	37.0	37.0	33.0	37.0
23	35.274370729639756	37.0	37.0	37.0	33.0	37.0
24	35.25808467887964	37.0	37.0	37.0	33.0	37.0
25	35.249473540197386	37.0	37.0	37.0	33.0	37.0
26	35.17520313971237	37.0	37.0	37.0	33.0	37.0
27	35.13510098639118	37.0	37.0	37.0	33.0	37.0
28	35.14389454171842	37.0	37.0	37.0	33.0	37.0
29	35.17198402244798	37.0	37.0	37.0	33.0	37.0
30	35.15167675770509	37.0	37.0	37.0	33.0	37.0
31	35.12540876082763	37.0	37.0	37.0	33.0	37.0
32	35.17606961877604	37.0	37.0	37.0	33.0	37.0
33	35.15100342567729	37.0	37.0	37.0	33.0	37.0
34	35.095782151604325	37.0	37.0	37.0	33.0	37.0
35	35.078763751666564	37.0	37.0	37.0	33.0	37.0
36	35.071005679059375	37.0	37.0	37.0	33.0	37.0
37	35.079402209924005	37.0	37.0	37.0	33.0	37.0
38	34.94050803035628	37.0	37.0	37.0	27.0	37.0
39	35.006566999219366	37.0	37.0	37.0	27.0	37.0
40	35.05719834859284	37.0	37.0	37.0	27.0	37.0
41	35.07190166669796	37.0	37.0	37.0	33.0	37.0
42	34.95448972967463	37.0	37.0	37.0	27.0	37.0
43	34.778441571680354	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	14.0
16	21.0
17	15.0
18	13.0
19	16.0
20	24.0
21	56.0
22	176.0
23	442.0
24	928.0
25	1786.0
26	2898.0
27	4410.0
28	6521.0
29	8612.0
30	11229.0
31	14625.0
32	18532.0
33	24131.0
34	34765.0
35	63369.0
36	180188.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.29449557773766	21.432346226792177	13.875736708398945	24.39742148707122
2	15.41635257918358	23.691630026852803	38.68279086736432	22.2092265265993
3	17.38672060476483	27.360618928946035	31.54010617721777	23.712554289071367
4	12.503319714678906	18.34360321160599	39.80062933742519	29.352447736289918
5	12.994503357271048	38.044332609926144	36.07611066252116	12.885053370281646
6	28.593809100981026	40.46430401343445	18.148846617110145	12.793040268474378
7	25.045000576758508	33.00507279228914	24.570449040032404	17.379477590919944
8	23.575741805334612	36.947954921627904	21.45809916490733	18.01820410813015
9	25.40420041151049	15.994988907458426	20.825006102909814	37.77580457812127
10	14.093831903061648	28.96266628752619	35.012997185954994	21.93050462345717
11	32.737886059344426	24.238611702027775	25.058681825132183	17.964820413495612
12	21.578547802550077	26.36778951265247	31.436021385669026	20.617641299128426
13	26.861119233420876	22.91367668795755	27.57254414885198	22.65265992976959
14	21.228737059819245	21.882754384035323	28.476847840374703	28.411660715770726
15	23.141965753957503	29.633852237152368	25.09704297253288	22.127139036357246
16	22.317335214728534	29.564104696423826	25.64804854428835	22.47051154455929
17	21.73601628873336	28.679920487803567	27.089140039648797	22.49492318381428
18	21.68611997113525	28.302210728781326	28.54713190064731	21.464537399436118
19	23.635295474725908	27.30160177909881	28.55410665472016	20.508996091455124
20	23.258390495019757	26.929793735061285	28.768177952802375	21.043637817116583
21	22.025468582756798	28.066142129392418	27.741547805232674	22.166841482618107
22	21.50719070318934	28.112014550410038	27.945156972205602	22.43563777419502
23	21.651782720315044	27.99290721162745	28.38402995925134	21.971280108806162
24	22.57245025793177	28.007393239317224	27.453168550297363	21.966987952453636
25	22.49599622290241	27.62351350553822	28.175323856609786	21.70516641494958
26	22.377693663435924	27.76944682152409	27.851266051994113	22.001593463045875
27	22.207885227739133	27.792785421690947	27.590249293806153	22.409080056763766
28	21.555209202383217	27.911892760473535	28.522183741848256	22.010714295294992
29	22.694508454206716	27.996394588663883	27.574958486800277	21.73413847032913
30	22.148599818119873	28.000955004788437	28.007124979545196	21.843320197546497
31	22.511555289680317	27.734304791387785	28.02965880039595	21.724481118535945
32	21.501825507748684	27.66160639316689	28.44921708385532	22.387351015229108
33	21.7126776885665	27.617611790553497	28.551960576543898	22.117749944336097
34	21.91628685553943	27.58193324087313	28.148766139178534	22.353013764408903
35	22.015811230963617	28.200540275180874	27.863874261279655	21.919774232575858
36	22.399690964742618	27.944083933117476	27.61358789397301	22.0426372081669
37	22.593911039694397	27.10174824893434	27.837048284076367	22.467292427294897
38	22.654806007945854	27.521843051937772	27.882652445321952	21.940698494794418
39	21.98335179854764	27.61171007556878	28.283700804511057	22.121237321372526
40	21.803617751285636	27.87299509352877	28.433389757305385	21.88999739788021
41	21.74352756235028	27.516209596725083	28.4081733387343	22.332089502190343
42	22.224785593377202	27.624854804398385	27.887749380990577	22.26261022123383
43	22.202520032298477	27.019392498920254	28.212880224694388	22.565207244086885
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	22.5
2	30.0
3	102.0
4	174.0
5	174.0
6	183.5
7	193.0
8	186.5
9	180.0
10	274.5
11	369.0
12	369.0
13	571.5
14	774.0
15	1377.5
16	1981.0
17	2365.5
18	2750.0
19	2750.0
20	2931.0
21	3112.0
22	3909.0
23	4706.0
24	5860.5
25	7015.0
26	7015.0
27	8793.0
28	10571.0
29	13031.0
30	15491.0
31	17052.5
32	18614.0
33	18614.0
34	20163.5
35	21713.0
36	23409.0
37	25105.0
38	26569.5
39	28034.0
40	28034.0
41	29067.0
42	30100.0
43	29029.5
44	27959.0
45	27592.5
46	27226.0
47	27226.0
48	26621.0
49	26016.0
50	26041.0
51	26066.0
52	25603.5
53	25141.0
54	25141.0
55	22206.0
56	19271.0
57	17031.5
58	14792.0
59	12981.0
60	11170.0
61	11170.0
62	9835.5
63	8501.0
64	7033.5
65	5566.0
66	4694.5
67	3823.0
68	3823.0
69	3083.0
70	2343.0
71	2033.5
72	1724.0
73	1394.5
74	1065.0
75	1065.0
76	768.0
77	471.0
78	376.5
79	282.0
80	231.5
81	181.0
82	181.0
83	135.5
84	90.0
85	90.0
86	90.0
87	68.5
88	47.0
89	47.0
90	31.5
91	16.0
92	11.0
93	6.0
94	3.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	372773.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.11262932281531
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.28469714299348	58.78674018594376
2	5.29548877980177	6.8960639600472025
3	1.4328516647957157	2.7989021797326674
4	0.6828961192928126	1.7786064752600788
5	0.40505770015371156	1.3187185942230346
6	0.2854370265957404	1.1151333184641012
7	0.1827393708997924	0.832904864405785
8	0.15055386249942546	0.7842366273634555
9	0.12195382572937924	0.7146660824294657
>10	0.9762087456122053	13.074934194550586
>50	0.13150500326990808	5.868782458385325
>100	0.04936623151125158	5.337736924557318
>500	8.296845632143122E-4	0.367356015252161
>1k	4.148422816071561E-4	0.32521811938500134
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1204	0.32298476552754624	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	845	0.22667950736775466	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	515	0.13815378259691555	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	418	0.11213258470972952	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	392	0.10515783063687553	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.682597720328457E-4	0.0	0.0
2	0.0	0.0	2.682597720328457E-4	0.0	0.0
3	0.0	0.0	2.682597720328457E-4	0.0	0.0
4	0.0	0.0	2.682597720328457E-4	2.682597720328457E-4	0.0
5	0.0	0.0	2.682597720328457E-4	2.682597720328457E-4	0.0
6	0.0	0.0	5.365195440656914E-4	2.682597720328457E-4	0.0
7	0.0	0.0	8.047793160985371E-4	2.682597720328457E-4	0.0
8	0.0	0.0	0.0021460781762627657	2.682597720328457E-4	0.0
9	0.0	0.0	0.002950857492361303	0.0010730390881313829	0.0
10	0.0	0.0	0.00375563680845984	0.0010730390881313829	0.0
11	0.0	0.0	0.005901714984722606	0.0013412988601642286	0.0
12	0.0	0.0	0.006438234528788297	0.0013412988601642286	0.0
13	0.0	0.0	0.00751127361691968	0.0013412988601642286	0.0
14	0.0	0.0	0.008316052933018217	0.0013412988601642286	0.0
15	0.0	0.0	0.009389092021149601	0.0013412988601642286	0.0
16	0.0	0.0	0.010193871337248138	0.0013412988601642286	0.0
17	0.0	0.0	0.010730390881313829	0.0016095586321970743	0.0
18	0.0	0.0	0.010998650653346674	0.0016095586321970743	0.0
19	0.0	0.0	0.011266910425379521	0.0016095586321970743	0.0
20	0.0	0.0	0.011266910425379521	0.0016095586321970743	0.0
21	2.682597720328457E-4	0.0	0.011535170197412367	0.0016095586321970743	0.0
22	2.682597720328457E-4	0.0	0.011535170197412367	0.0021460781762627657	0.0
23	2.682597720328457E-4	0.0	0.011803429969445212	0.002950857492361303	0.0
24	2.682597720328457E-4	0.0	0.012071689741478058	0.0032191172643941486	0.0
25	2.682597720328457E-4	0.0	0.012071689741478058	0.0032191172643941486	0.0
26	2.682597720328457E-4	0.0	0.012071689741478058	0.0034873770364269945	0.0
27	2.682597720328457E-4	0.0	0.012071689741478058	0.004560416124558377	0.0
28	2.682597720328457E-4	0.0	0.012071689741478058	0.01314472882960944	0.0
29	2.682597720328457E-4	0.0	0.012071689741478058	0.031118133555810105	0.0
30	2.682597720328457E-4	0.0	0.012071689741478058	0.05204239577437207	0.0
31	2.682597720328457E-4	0.0	0.012071689741478058	0.11240084448176237	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATAC	20	0.001840341	37.0	24
GCGCATA	20	0.001840341	37.0	23
GGTATCA	210	0.0	23.785713	1
GTATCAA	595	0.0	22.386555	1
CGAACTA	90	9.43528E-8	20.555555	29
GCGAACT	90	9.43528E-8	20.555555	28
GTCTAGA	45	0.0038210328	20.555555	1
ACGCTTC	45	0.0038210328	20.555555	25
CGCGCGC	55	5.1341375E-4	20.181818	10
TAGATCT	55	5.1341375E-4	20.181818	4
ATCGCGC	55	5.1341375E-4	20.181818	8
TCGCGCG	55	5.1341375E-4	20.181818	9
CGTGACG	75	9.238256E-6	19.733334	36
GTGACGT	75	9.238256E-6	19.733334	37
CGAACCG	60	9.220861E-4	18.5	31
GCGAACC	50	0.007026185	18.5	30
GGCCGTT	50	0.007026185	18.5	14
ACACACG	50	0.007026185	18.5	6
GCGCCTT	50	0.007026185	18.5	13
CGCCTTT	50	0.007026185	18.5	14
>>END_MODULE