FastQCFastQC Report
Fri 10 Feb 2017
ERR1632853.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632853.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252398
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6300.24960578134533554No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4760.18859103479425351No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2860.11331310073772376No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCA200.001839215837.015
CTAGCCT200.001839215837.04
TTGCCGA358.850148E-426.4285710
CCGAGTT451.319218E-424.66666813
GTATCAA3700.023.51
TTTGCCG400.001927036223.1259
CTCTAGC400.001927036223.1252
CGAGTTC502.6940124E-422.214
GCCGAGT502.6940124E-422.212
ACCAATG806.915925E-720.81257
TATTATT450.00381766220.5555572
TTCATAA450.00381766220.5555572
GGATTCA450.00381766220.55555713
GTAAGAT450.00381766220.5555573
GGGTAAG555.1278214E-420.1818181
GGTATCA1101.7353159E-920.1818181
TCAACGC4750.018.6947364
GCCCAAT500.007020047418.512
ATAATAT902.138704E-618.53
GATTTGC500.007020047418.57
Produced by FastQC (version 0.11.5)