FastQCFastQC Report
Fri 10 Feb 2017
ERR1632852.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632852.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374313
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19260.5145426421203645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14670.39191799376457664No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC11590.3096339160007801No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG9840.262881599089532No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG9580.25593554057700374No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9510.2540654479005538No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT9410.2513938869341968No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG8960.23937186258559012No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA8950.2391047064889544No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC8350.22307534069081225No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG8310.22200671630426944No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC8200.2190679992412767No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG8030.21452634559846973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7630.20384010173304162No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG7470.19956560418687036No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT7160.19128376519116355No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT7130.19048229690125645No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA7020.1875435798382637No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT6680.1784602725526498No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA6650.17765880426274266No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT6600.17632302377956416No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC6550.17498724329638563No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT6470.17284999452330002No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC6420.1715142140401215No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT6210.16590393601077172No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG6180.16510246772086462No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA6150.16430099943095752No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC6130.16376668723768611No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG6110.16323237504441468No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC5850.1562863165318864No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA5760.15388191166216508No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG5670.15147750679244376No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG5580.14907310192272244No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA5520.14747016534290822No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT5450.1456000726664583No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG5400.14426429218327977No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT5330.14239419950682985No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5300.14159273121692273No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC5290.14132557512028704No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG5220.13945548244383713No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC5070.13544814099430155No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT5020.13411236051112305No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT4830.12903639467504469No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGC4810.12850208248177328No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA4760.12716630199859474No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT4690.12529620932214483No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG4600.12289180445242351No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA4590.1226246483557878No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT4530.12102171177597358No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC4480.11968593129279506No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4450.11888446300288796No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4440.11861730690625226No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG4440.11861730690625226No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT4420.11808299471298084No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG4360.11648005813316663No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4240.1132741849735382No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC4230.11300702887690249No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG4230.11300702887690249No Hit
ACCTTAGCCAAACCATTTACCCAGATAAAGTATAGGCGATAGA4170.11140409229708827No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG4170.11140409229708827No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA4160.11113693620045256No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG4110.10980115571727406No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4050.10819821913745982No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC4020.10739675084755272No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC4010.10712959475091702No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG3960.10579381426773848No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTAC3910.10445803378455998No Hit
GATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGAT3840.10258794110811005No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGA3840.10258794110811005No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT3820.10205362891483866No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA3810.10178647281820295No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA3780.10098500452829584No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCTT200.001840350837.08
TTAAGTT200.001840350837.017
GAACGTA200.001840350837.018
GATCTTG200.001840350837.09
GGTATCA2800.029.7321411
AACGTAT250.00549166829.619
ATAGATC250.00549166829.66
CTAAGGA250.00549166829.623
CGGTGTA250.00549166829.634
ACGTATT250.00549166829.620
GTGACAT250.00549166829.616
GATTATC250.00549166829.67
TCGGTGT250.00549166829.633
GTCGGTG250.00549166829.632
GTATCAA10200.026.1176471
GGTCAGA509.069719E-625.90000219
ACTCGCA451.3208849E-424.66666421
TACTCGC451.3208849E-424.66666420
TATAGAT451.3208849E-424.6666645
ATTATTT451.3208849E-424.66666419
Produced by FastQC (version 0.11.5)