FastQCFastQC Report
Fri 10 Feb 2017
ERR1632851.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632851.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291221
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8650.29702528320416455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6270.21530040759423252No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3350.11503291314843367No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGGG251.2313634E-436.99999620
ATTGACG250.005489690729.59999817
GGTATCA1650.028.0303021
CACGCGG358.8534347E-426.4285719
GTATCAA4850.021.3608251
AACCGTG450.00381905420.5555558
ACCCGCA450.00381905420.55555526
ACGGGAC609.214259E-418.55
CGGGAAT500.007022583418.49999823
TCTATAC500.007022583418.4999983
CAACGCA5850.018.0256425
TCAACGC5900.017.8728834
ATCAACG5950.017.7226893
TATCAAC6050.017.1239662
GGCGGAC650.00157610717.07692313
CTTAGGC650.00157610717.0769233
AACGCAG6150.016.845536
GTATTAG803.37173E-416.18751
ACCTAAA803.37173E-416.18751
ACGCAGA6500.015.9384627
Produced by FastQC (version 0.11.5)