##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632851.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	291221
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.659035577791435	33.0	33.0	33.0	27.0	33.0
2	31.19791841934476	33.0	33.0	33.0	27.0	33.0
3	31.621064414997544	33.0	33.0	33.0	27.0	33.0
4	31.765507981910645	33.0	33.0	33.0	27.0	33.0
5	31.86669230584333	33.0	33.0	33.0	33.0	33.0
6	34.73344298659781	37.0	37.0	37.0	27.0	37.0
7	34.87689761383966	37.0	37.0	37.0	27.0	37.0
8	35.06189114109216	37.0	37.0	37.0	33.0	37.0
9	35.11142053629374	37.0	37.0	37.0	33.0	37.0
10	35.2102355255974	37.0	37.0	37.0	33.0	37.0
11	35.21255335295188	37.0	37.0	37.0	33.0	37.0
12	35.141013182428466	37.0	37.0	37.0	33.0	37.0
13	35.20058649616614	37.0	37.0	37.0	33.0	37.0
14	35.1396568241988	37.0	37.0	37.0	33.0	37.0
15	35.17572221783457	37.0	37.0	37.0	33.0	37.0
16	35.16731966444728	37.0	37.0	37.0	33.0	37.0
17	35.16935935251922	37.0	37.0	37.0	33.0	37.0
18	35.126886453930176	37.0	37.0	37.0	33.0	37.0
19	35.17092173984706	37.0	37.0	37.0	33.0	37.0
20	35.06300713204062	37.0	37.0	37.0	33.0	37.0
21	35.14733484192418	37.0	37.0	37.0	33.0	37.0
22	35.16712393680401	37.0	37.0	37.0	33.0	37.0
23	35.13272394504517	37.0	37.0	37.0	33.0	37.0
24	35.13327678979194	37.0	37.0	37.0	33.0	37.0
25	35.14551148440531	37.0	37.0	37.0	33.0	37.0
26	35.070812201043196	37.0	37.0	37.0	33.0	37.0
27	35.03640534164775	37.0	37.0	37.0	27.0	37.0
28	35.01874521411574	37.0	37.0	37.0	27.0	37.0
29	35.062389044746084	37.0	37.0	37.0	33.0	37.0
30	35.043115022611694	37.0	37.0	37.0	27.0	37.0
31	35.0241946837625	37.0	37.0	37.0	27.0	37.0
32	35.045350438326906	37.0	37.0	37.0	27.0	37.0
33	35.0165956438581	37.0	37.0	37.0	27.0	37.0
34	34.95222871976952	37.0	37.0	37.0	27.0	37.0
35	34.96138327936516	37.0	37.0	37.0	27.0	37.0
36	34.94433437149107	37.0	37.0	37.0	27.0	37.0
37	34.96441190710835	37.0	37.0	37.0	27.0	37.0
38	34.82557919930225	37.0	37.0	37.0	27.0	37.0
39	34.887102921836	37.0	37.0	37.0	27.0	37.0
40	34.92940412950989	37.0	37.0	37.0	27.0	37.0
41	34.94284409434759	37.0	37.0	37.0	27.0	37.0
42	34.81123270643257	37.0	37.0	37.0	27.0	37.0
43	34.64951016581909	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	6.0
16	22.0
17	14.0
18	7.0
19	14.0
20	26.0
21	76.0
22	195.0
23	427.0
24	946.0
25	1681.0
26	2648.0
27	3824.0
28	5396.0
29	7350.0
30	9367.0
31	11708.0
32	14685.0
33	19858.0
34	27518.0
35	50388.0
36	135065.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.020808938915806	21.133778127264176	13.629854989853065	25.215557943966953
2	15.632801205956989	23.1308868522531	37.93236064706872	23.30395129472119
3	17.689658369417042	26.74017327047157	30.574718169362786	24.995450190748606
4	12.809515797281101	18.052269582207327	39.288718876729355	29.84949574378221
5	13.88121048962815	37.0093502872389	35.735060315018494	13.374378908114457
6	29.79901861472902	39.69150576366402	17.553335782790388	12.956139838816568
7	26.15882783178411	31.98292705539779	24.267824092355976	17.590421020462124
8	24.39384522407381	36.25494040608335	21.028359905363967	18.322854464478866
9	25.832615093004968	15.243749592234076	20.658194292307215	38.265441022453736
10	14.302883377228978	28.714962176491394	34.412696886556944	22.569457559722686
11	33.69914944320636	23.669996325814417	24.669237451969465	17.961616779009756
12	22.226075729428853	25.388622386435046	31.64503933438866	20.74026254974744
13	28.001758114971103	22.334584387801705	26.55062650014937	23.11303099707782
14	21.82363222432448	20.82130066169679	27.989052987250236	29.366014126728494
15	23.199563218311866	29.470058821307532	24.288427002173606	23.041950958206996
16	22.874037243193314	28.545674934156533	25.49060679003231	23.08968103261784
17	21.86998877141415	28.131899828652468	27.120296956606836	22.877814443326546
18	23.016540702765255	27.51999340706886	27.77581287063776	21.687653019528124
19	23.878429096802773	27.019686080330747	27.527547807335324	21.57433701553116
20	23.622266251403573	27.135749138970063	27.42693693105923	21.815047678567137
21	23.092771469090483	26.98500451547107	27.556391881080007	22.365832134358442
22	22.468159919786004	26.864134111207637	27.81427163563067	22.853434333375684
23	22.574951669007387	26.943455314005515	27.60343519183026	22.87815782515684
24	22.501124575494213	27.207859323331768	27.26383056166966	23.027185539504362
25	22.992160592814393	27.416978857980705	27.479817732924477	22.111042816280417
26	22.987353247190278	27.151544703163577	27.364441437945754	22.496660611700392
27	22.855151242527153	27.373712747363687	27.237390160737036	22.533745849372128
28	22.698569127913164	27.096603610316567	27.514155915953864	22.69067134581641
29	22.928291572379738	27.059861754475122	27.348989255582527	22.662857417562606
30	22.95335844599119	27.1000374286195	27.4118281305263	22.534775994863008
31	22.954045209651778	26.919075204054653	27.66696083043462	22.45991875585895
32	22.599675160788543	26.989125097434595	27.690654176724895	22.72054556505197
33	22.761064621026645	27.022776516803386	27.556735262910298	22.659423599259668
34	22.900821025956233	26.72884167007187	27.42968398570158	22.940653318270318
35	22.699942655234338	27.170087321999443	27.506258133857102	22.62371188890911
36	23.15251990756161	26.99049862475577	27.340061327994892	22.51692013968773
37	22.9804856105844	26.60900141129932	27.500077260911816	22.91043571720446
38	22.858241678999796	27.13952633910329	27.511408861311516	22.4908231205854
39	22.663200799392904	26.860013529244114	27.864748764683867	22.61203690667912
40	22.900134262295644	27.033077971712206	27.742504833099265	22.32428293289289
41	22.768962403123403	26.569855882645825	27.708510031900175	22.9526716823306
42	22.422490136356924	27.0111015345734	27.534758825771494	23.031649503298183
43	22.680026509077297	26.595952901748156	27.7682584703713	22.955762118803246
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	15.5
2	23.0
3	59.5
4	96.0
5	96.0
6	126.0
7	156.0
8	147.5
9	139.0
10	192.0
11	245.0
12	245.0
13	378.0
14	511.0
15	898.5
16	1286.0
17	1569.0
18	1852.0
19	1852.0
20	1948.0
21	2044.0
22	2477.0
23	2910.0
24	3842.5
25	4775.0
26	4775.0
27	5961.0
28	7147.0
29	8599.5
30	10052.0
31	11334.5
32	12617.0
33	12617.0
34	14220.0
35	15823.0
36	17147.0
37	18471.0
38	19382.5
39	20294.0
40	20294.0
41	21207.0
42	22120.0
43	22283.0
44	22446.0
45	22430.5
46	22415.0
47	22415.0
48	21896.0
49	21377.0
50	21095.5
51	20814.0
52	19784.0
53	18754.0
54	18754.0
55	17743.5
56	16733.0
57	14797.5
58	12862.0
59	12045.0
60	11228.0
61	11228.0
62	9989.0
63	8750.0
64	7178.0
65	5606.0
66	4648.5
67	3691.0
68	3691.0
69	3068.0
70	2445.0
71	1978.5
72	1512.0
73	1192.5
74	873.0
75	873.0
76	671.0
77	469.0
78	361.5
79	254.0
80	211.0
81	168.0
82	168.0
83	141.0
84	114.0
85	98.5
86	83.0
87	59.0
88	35.0
89	35.0
90	28.0
91	21.0
92	11.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	291221.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.35626208126844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.31978651478359	67.3080524165098
2	6.085349377085202	9.171383650314324
3	1.7847514507612043	4.0347659422045625
4	0.815853078272128	2.459185535443364
5	0.5024967810137857	1.8933139562534296
6	0.3042217181078326	1.3755006912328565
7	0.21744846870441312	1.1470272657802179
8	0.14454228248318257	0.8713732896501954
9	0.12579612320094954	0.8531573065864458
>10	0.6700221670884697	8.596453764390153
>50	0.025157878730102064	1.287767751706679
>100	0.003659327815287302	0.4877385890430533
>500	9.148319538218255E-4	0.5142798408849721
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	865	0.29702528320416455	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	627	0.21530040759423252	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	335	0.11503291314843367	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.433818302938318E-4	0.0	0.0
2	0.0	0.0	3.433818302938318E-4	0.0	0.0
3	0.0	0.0	3.433818302938318E-4	0.0	0.0
4	0.0	0.0	3.433818302938318E-4	0.0	0.0
5	0.0	0.0	0.0010301454908814955	0.0	0.0
6	0.0	0.0	0.002060290981762991	0.0	0.0
7	0.0	0.0	0.004120581963525982	0.0	0.0
8	0.0	0.0	0.005150727454407478	0.0	0.0
9	0.0	0.0	0.0061808729452889725	3.433818302938318E-4	0.0
10	3.433818302938318E-4	0.0	0.006524254775582805	3.433818302938318E-4	0.0
11	3.433818302938318E-4	0.0	0.008241163927051964	3.433818302938318E-4	0.0
12	3.433818302938318E-4	0.0	0.009614691248227291	3.433818302938318E-4	0.0
13	3.433818302938318E-4	0.0	0.009614691248227291	3.433818302938318E-4	0.0
14	3.433818302938318E-4	0.0	0.010301454908814955	3.433818302938318E-4	0.0
15	3.433818302938318E-4	0.0	0.010988218569402618	3.433818302938318E-4	0.0
16	3.433818302938318E-4	0.0	0.012018364060284115	3.433818302938318E-4	0.0
17	3.433818302938318E-4	0.0	0.012361745890577945	3.433818302938318E-4	0.0
18	3.433818302938318E-4	0.0	0.012361745890577945	3.433818302938318E-4	0.0
19	3.433818302938318E-4	0.0	0.012705127720871777	3.433818302938318E-4	0.0
20	3.433818302938318E-4	0.0	0.01304850955116561	6.867636605876636E-4	0.0
21	3.433818302938318E-4	0.0	0.013391891381459442	6.867636605876636E-4	0.0
22	3.433818302938318E-4	0.0	0.013735273211753274	0.0010301454908814955	0.0
23	6.867636605876636E-4	0.0	0.013735273211753274	0.0010301454908814955	0.0
24	6.867636605876636E-4	0.0	0.013735273211753274	0.0013735273211753273	0.0
25	6.867636605876636E-4	0.0	0.013735273211753274	0.0013735273211753273	0.0
26	6.867636605876636E-4	0.0	0.013735273211753274	0.0013735273211753273	0.0
27	6.867636605876636E-4	0.0	0.013735273211753274	0.002060290981762991	0.0
28	6.867636605876636E-4	0.0	0.013735273211753274	0.008927927587639628	0.0
29	6.867636605876636E-4	0.0	0.013735273211753274	0.025066873611449724	0.0
30	6.867636605876636E-4	0.0	0.013735273211753274	0.04292272878672898	0.0
31	6.867636605876636E-4	0.0	0.013735273211753274	0.09649029431256674	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGGG	25	1.2313634E-4	36.999996	20
ATTGACG	25	0.0054896907	29.599998	17
GGTATCA	165	0.0	28.030302	1
CACGCGG	35	8.8534347E-4	26.42857	19
GTATCAA	485	0.0	21.360825	1
AACCGTG	45	0.003819054	20.555555	8
ACCCGCA	45	0.003819054	20.555555	26
ACGGGAC	60	9.214259E-4	18.5	5
CGGGAAT	50	0.0070225834	18.499998	23
TCTATAC	50	0.0070225834	18.499998	3
CAACGCA	585	0.0	18.025642	5
TCAACGC	590	0.0	17.872883	4
ATCAACG	595	0.0	17.722689	3
TATCAAC	605	0.0	17.123966	2
GGCGGAC	65	0.001576107	17.076923	13
CTTAGGC	65	0.001576107	17.076923	3
AACGCAG	615	0.0	16.84553	6
GTATTAG	80	3.37173E-4	16.1875	1
ACCTAAA	80	3.37173E-4	16.1875	1
ACGCAGA	650	0.0	15.938462	7
>>END_MODULE