FastQCFastQC Report
Fri 10 Feb 2017
ERR1632850.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632850.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354182
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19600.5533878062690933No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15210.4294402312935158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7310.20639106448097308No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC6070.17138081551292839No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG5690.1606518682485276No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG5630.1589578239436222No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC5420.15302866887645336No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5270.14879355811418987No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG5270.14879355811418987No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG5100.14399376591695795No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA4810.13580588510991523No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG4800.135523544392431No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT4750.13411184080500985No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC4660.13157077434765177No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4570.1290297078902937No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT4220.11914778277834559No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4200.11858310134337713No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC4090.11547735345105059No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT4040.11406564986362942No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT3990.11265394627620827No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG3930.11095990197130289No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA3910.11039522053633442No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA3890.10983053910136595No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT3850.10870117623142904No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3750.10587776905658672No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC3740.10559542833910249No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGTA200.001840217537.013
GGTATCA2500.033.31
CCCACGA250.00549127429.59999819
GTAGAAC250.00549127429.59999816
ATTGACT358.857232E-426.42857234
AGTGACA1500.025.923
AGGCACC1300.025.6153858
GTGACAC1450.025.5172424
GTATCAA8950.024.8044681
GCACCGC1350.024.66666810
GACACAT1500.024.66666626
TCCTTGT451.3206885E-424.6666661
GCCAATC451.3206885E-424.66666622
CACCGCC1550.023.87096811
CTTGTAC551.8978439E-523.5454543
ATTAGAG1650.023.5454543
GCCAACC551.8978439E-523.54545412
CACATGT1600.023.12528
GGCCAGC400.001928564523.12531
CGCTCTA400.001928564523.12514
Produced by FastQC (version 0.11.5)