##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632850.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	354182
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.719621550502286	33.0	33.0	33.0	27.0	33.0
2	31.23166055869581	33.0	33.0	33.0	27.0	33.0
3	31.66230920826016	33.0	33.0	33.0	27.0	33.0
4	31.80411201020944	33.0	33.0	33.0	27.0	33.0
5	31.915560361621992	33.0	33.0	33.0	33.0	33.0
6	34.79447289811453	37.0	37.0	37.0	27.0	37.0
7	34.96309242141046	37.0	37.0	37.0	27.0	37.0
8	35.1208587675263	37.0	37.0	37.0	33.0	37.0
9	35.181629783557604	37.0	37.0	37.0	33.0	37.0
10	35.19638773286051	37.0	37.0	37.0	33.0	37.0
11	35.24165259668758	37.0	37.0	37.0	33.0	37.0
12	35.17228995262323	37.0	37.0	37.0	33.0	37.0
13	35.22408253383853	37.0	37.0	37.0	33.0	37.0
14	35.167501454054694	37.0	37.0	37.0	33.0	37.0
15	35.215634899571405	37.0	37.0	37.0	33.0	37.0
16	35.22544906291116	37.0	37.0	37.0	33.0	37.0
17	35.196559960698174	37.0	37.0	37.0	33.0	37.0
18	35.19974758739857	37.0	37.0	37.0	33.0	37.0
19	35.200046868559106	37.0	37.0	37.0	33.0	37.0
20	35.18688132090281	37.0	37.0	37.0	33.0	37.0
21	35.20358459774918	37.0	37.0	37.0	33.0	37.0
22	35.19791237273492	37.0	37.0	37.0	33.0	37.0
23	35.20206278128194	37.0	37.0	37.0	33.0	37.0
24	35.16893292149234	37.0	37.0	37.0	33.0	37.0
25	35.17844780367156	37.0	37.0	37.0	33.0	37.0
26	35.14909283927472	37.0	37.0	37.0	33.0	37.0
27	35.08167552275384	37.0	37.0	37.0	33.0	37.0
28	35.058859569373936	37.0	37.0	37.0	27.0	37.0
29	35.09028126782276	37.0	37.0	37.0	33.0	37.0
30	35.02939731550446	37.0	37.0	37.0	27.0	37.0
31	35.00289681576139	37.0	37.0	37.0	27.0	37.0
32	35.043813632539205	37.0	37.0	37.0	27.0	37.0
33	35.01369070139081	37.0	37.0	37.0	27.0	37.0
34	34.953286728292234	37.0	37.0	37.0	27.0	37.0
35	34.94165146732471	37.0	37.0	37.0	27.0	37.0
36	34.925061126765335	37.0	37.0	37.0	27.0	37.0
37	34.94439017228431	37.0	37.0	37.0	27.0	37.0
38	34.799840195153905	37.0	37.0	37.0	27.0	37.0
39	34.86764149505057	37.0	37.0	37.0	27.0	37.0
40	34.88301212371041	37.0	37.0	37.0	27.0	37.0
41	34.90283808889215	37.0	37.0	37.0	27.0	37.0
42	34.803346866865056	37.0	37.0	37.0	27.0	37.0
43	34.582395491583426	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	13.0
16	14.0
17	24.0
18	14.0
19	14.0
20	33.0
21	74.0
22	215.0
23	498.0
24	1028.0
25	1901.0
26	3112.0
27	4534.0
28	6377.0
29	8660.0
30	11305.0
31	14335.0
32	18210.0
33	23947.0
34	33645.0
35	60258.0
36	165971.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.991868587336455	21.805455952024666	13.749145919329612	24.453529541309273
2	16.364467985386042	23.336589662941652	37.842691045846486	22.45625130582582
3	16.62704485264638	28.942746949308546	30.07352152283289	24.35668667521218
4	11.658412906358878	18.6929318824785	41.6938184323314	27.954836778831222
5	12.389675364643036	38.3401753900537	36.602650614655744	12.66749863064752
6	27.137460401714375	42.49651309213907	17.56272199038912	12.803304515757436
7	25.746650027387048	32.37883348109164	25.01934033914767	16.855176152373637
8	23.918776222394136	35.58735339458244	20.793829161278666	19.70004122174475
9	26.004144761732668	14.893190506575715	20.8200868480047	38.28257788368692
10	15.261080461457668	27.714282487534657	34.821080687330245	22.203556363677432
11	32.69731381041385	25.10460723582791	24.35471029018979	17.843368663568448
12	22.477426859637138	27.75522189156987	29.361740574055144	20.405610674737847
13	27.437870925117597	23.22901784958016	25.90476082917822	23.428350396124024
14	21.944367585026907	21.551631647006342	28.360842730573548	28.143158037393206
15	23.743724977553914	29.26659175226296	25.233636943718203	21.756046326464926
16	22.19282741641303	28.63414854509828	25.888949748999103	23.284074289489585
17	21.69957818296808	27.73941081139075	26.64392882755194	23.91708217808923
18	22.083843899464117	27.835688996052877	28.29985713559695	21.780609968886054
19	24.49559830821442	26.037743307113292	28.681299444918153	20.785358939754136
20	23.634459119887516	26.831967745396433	28.431427909944606	21.102145224771444
21	22.08073815157179	27.857711572016647	28.525447368866853	21.53610290754471
22	21.655250690323054	27.336792948258243	28.529117798194147	22.478838563224556
23	21.86446516197887	27.139154446019276	29.403527000242814	21.59285339175904
24	21.51746842019075	28.585585941690994	26.767876402527513	23.12906923559074
25	22.286564534617796	27.656120299732905	28.906042656035595	21.1512725096137
26	21.801220841262403	27.93479058788984	28.85550366760592	21.40848490324184
27	21.53497354467477	27.88255755515526	27.67362542421693	22.90884347595304
28	21.525091619562826	27.21453941758757	29.86713045835192	21.39323850449769
29	23.672010435312917	26.83761455974612	27.967259770400528	21.523115234540434
30	22.937642229136433	27.044570305662063	28.151063577482766	21.866723887718745
31	22.837693615147014	27.146495304673866	28.396700001694043	21.619111078485073
32	21.141672925219236	26.98725514001276	28.808352767786054	23.062719166981946
33	21.03579515616265	27.250114347990582	29.46987706885161	22.24421342699516
34	22.60899763398479	26.320366365315007	28.877808584287173	22.19282741641303
35	22.196780186457808	27.802655132107223	28.346161013264364	21.654403668170602
36	23.48707726536075	27.487845232112303	26.988384502882703	22.03669299964425
37	22.32552755363062	26.438949466658386	28.123676527886794	23.111846451824203
38	22.3133869027788	27.87860478511048	27.691130548700947	22.11687776340977
39	21.5075864950788	27.43194177005043	28.790282961867064	22.27018877300371
40	21.6956254129233	27.820160256591244	29.29172007611906	21.192494254366398
41	21.82239639507372	26.608353897148923	28.337126110304872	23.232123597472487
42	21.096216069704276	28.284328396135322	28.383712328689768	22.235743205470634
43	22.590080805913342	26.145032779757297	28.693440095769972	22.571446318559385
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	27.0
2	28.0
3	125.0
4	222.0
5	222.0
6	272.5
7	323.0
8	300.0
9	277.0
10	374.0
11	471.0
12	471.0
13	736.0
14	1001.0
15	1778.5
16	2556.0
17	2687.0
18	2818.0
19	2818.0
20	2331.5
21	1845.0
22	1788.0
23	1731.0
24	2346.5
25	2962.0
26	2962.0
27	4189.5
28	5417.0
29	7196.5
30	8976.0
31	11509.5
32	14043.0
33	14043.0
34	17164.0
35	20285.0
36	22867.0
37	25449.0
38	27604.0
39	29759.0
40	29759.0
41	31967.5
42	34176.0
43	34770.0
44	35364.0
45	34270.5
46	33177.0
47	33177.0
48	30940.5
49	28704.0
50	27598.5
51	26493.0
52	24298.5
53	22104.0
54	22104.0
55	21176.5
56	20249.0
57	16131.0
58	12013.0
59	10800.0
60	9587.0
61	9587.0
62	8168.0
63	6749.0
64	4950.5
65	3152.0
66	2519.5
67	1887.0
68	1887.0
69	1452.5
70	1018.0
71	800.0
72	582.0
73	441.0
74	300.0
75	300.0
76	214.5
77	129.0
78	114.5
79	100.0
80	90.5
81	81.0
82	81.0
83	68.0
84	55.0
85	46.0
86	37.0
87	28.5
88	20.0
89	20.0
90	16.0
91	12.0
92	7.0
93	2.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	354182.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.241998808325185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.95179502321378	31.63774649019613
2	5.567351449845162	4.14678592121322
3	1.900597088851688	2.123461035543626
4	1.140387893219668	1.6988129824126137
5	0.7951968718505876	1.480736047692175
6	0.6731685184181141	1.504208469643766
7	0.5028058661060913	1.3107846826439375
8	0.4097645073078785	1.2208359434283171
9	0.3092046949953322	1.0363860794290243
>10	3.0330464115065063	23.2859144340095
>50	0.4566199699471108	12.071891838223001
>100	0.25250177146081954	16.21401168625194
>500	0.006047946621816036	1.2883591914478263
>1k	0.001511986655454009	0.9800651978649221
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1960	0.5533878062690933	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1521	0.4294402312935158	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	731	0.20639106448097308	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	607	0.17138081551292839	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	569	0.1606518682485276	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	563	0.1589578239436222	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	542	0.15302866887645336	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	527	0.14879355811418987	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	527	0.14879355811418987	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	510	0.14399376591695795	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	481	0.13580588510991523	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	480	0.135523544392431	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT	475	0.13411184080500985	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	466	0.13157077434765177	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	457	0.1290297078902937	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	422	0.11914778277834559	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	420	0.11858310134337713	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	409	0.11547735345105059	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	404	0.11406564986362942	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	399	0.11265394627620827	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	393	0.11095990197130289	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	391	0.11039522053633442	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	389	0.10983053910136595	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	385	0.10870117623142904	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	375	0.10587776905658672	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	374	0.10559542833910249	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	8.470221524526938E-4	0.0	0.0
2	0.0	0.0	0.0014117035874211563	2.8234071748423127E-4	0.0
3	0.0	0.0	0.0014117035874211563	2.8234071748423127E-4	0.0
4	0.0	0.0	0.0025410664573580813	2.8234071748423127E-4	0.0
5	0.0	0.0	0.0028234071748423125	2.8234071748423127E-4	0.0
6	0.0	0.0	0.003105747892326544	2.8234071748423127E-4	0.0
7	0.0	0.0	0.004799792197231932	2.8234071748423127E-4	0.0
8	0.0	0.0	0.005082132914716163	2.8234071748423127E-4	0.0
9	2.8234071748423127E-4	0.0	0.005929155067168857	5.646814349684625E-4	0.0
10	2.8234071748423127E-4	0.0	0.007058517937105782	5.646814349684625E-4	0.0
11	2.8234071748423127E-4	0.0	0.009881925111948095	5.646814349684625E-4	0.0
12	2.8234071748423127E-4	0.0	0.010446606546916558	5.646814349684625E-4	0.0
13	2.8234071748423127E-4	0.0	0.011575969416853482	5.646814349684625E-4	0.0
14	2.8234071748423127E-4	0.0	0.012422991569306176	5.646814349684625E-4	0.0
15	2.8234071748423127E-4	0.0	0.014399376591695795	5.646814349684625E-4	0.0
16	2.8234071748423127E-4	0.0	0.014964058026664258	5.646814349684625E-4	0.0
17	2.8234071748423127E-4	0.0	0.015246398744148489	5.646814349684625E-4	0.0
18	2.8234071748423127E-4	0.0	0.015811080179116952	8.470221524526938E-4	0.0
19	2.8234071748423127E-4	0.0	0.016375761614085414	0.001129362869936925	0.0
20	2.8234071748423127E-4	0.0	0.016375761614085414	0.001129362869936925	0.0
21	2.8234071748423127E-4	0.0	0.016940443049053875	0.001129362869936925	0.0
22	2.8234071748423127E-4	0.0	0.01722278376653811	0.0016940443049053876	0.0
23	2.8234071748423127E-4	0.0	0.01750512448402234	0.0016940443049053876	0.0
24	2.8234071748423127E-4	0.0	0.01750512448402234	0.001976385022389619	0.0
25	2.8234071748423127E-4	0.0	0.01750512448402234	0.0025410664573580813	0.0
26	2.8234071748423127E-4	0.0	0.01750512448402234	0.0028234071748423125	0.0
27	2.8234071748423127E-4	0.0	0.01750512448402234	0.004235110762263469	0.0
28	2.8234071748423127E-4	0.0	0.01750512448402234	0.016093420896601183	0.0
29	2.8234071748423127E-4	0.0	0.01750512448402234	0.03444556753307622	0.0
30	2.8234071748423127E-4	0.0	0.01750512448402234	0.06380900215143627	0.0
31	2.8234071748423127E-4	0.0	0.01750512448402234	0.1445584473519264	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGGGTA	20	0.0018402175	37.0	13
GGTATCA	250	0.0	33.3	1
CCCACGA	25	0.005491274	29.599998	19
GTAGAAC	25	0.005491274	29.599998	16
ATTGACT	35	8.857232E-4	26.428572	34
AGTGACA	150	0.0	25.9	23
AGGCACC	130	0.0	25.615385	8
GTGACAC	145	0.0	25.51724	24
GTATCAA	895	0.0	24.804468	1
GCACCGC	135	0.0	24.666668	10
GACACAT	150	0.0	24.666666	26
TCCTTGT	45	1.3206885E-4	24.666666	1
GCCAATC	45	1.3206885E-4	24.666666	22
CACCGCC	155	0.0	23.870968	11
CTTGTAC	55	1.8978439E-5	23.545454	3
ATTAGAG	165	0.0	23.545454	3
GCCAACC	55	1.8978439E-5	23.545454	12
CACATGT	160	0.0	23.125	28
GGCCAGC	40	0.0019285645	23.125	31
CGCTCTA	40	0.0019285645	23.125	14
>>END_MODULE