FastQCFastQC Report
Fri 10 Feb 2017
ERR1632849.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632849.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267823
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21870.8165840872516551No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17670.6597640979303495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7660.2860097900479048No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5060.18893074903947757No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3450.12881641979964378No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2950.11014737345186933No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.10454665954753699No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACC250.005488910729.63
TAGTCAC358.851566E-426.4285727
GGTATCA2750.025.5636351
GTATCAA8200.025.0426851
GTCACTC451.3195131E-424.6666669
CCATATA701.911485E-723.7857152
CCCTAGA400.001927342823.1251
GGCGAGT400.001927342823.12519
GTCCGGC450.003818264220.55555515
CCAACGG450.003818264220.55555529
TTCGCCG450.003818264220.55555524
GCGAGTC450.003818264220.55555520
TTGTCCG555.12895E-420.18181813
CTTCGCC500.00702114318.523
CGAACTA500.00702114318.529
AGTCACT701.2147715E-418.58
CACGGTA500.00702114318.52
CGAGTCG609.21162E-418.521
GAGTCGC609.21162E-418.522
CGTTCGC500.00702114318.534
Produced by FastQC (version 0.11.5)