FastQCFastQC Report
Fri 10 Feb 2017
ERR1632848.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632848.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322646
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7200.22315478883978107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5210.16147728470211933No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5210.16147728470211933No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG4480.13885186861141932No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4380.13575249654420016No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG3870.11994569900138231No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC3650.11312708045350013No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG3580.1109575200064467No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA3380.10475877587200833No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC3340.10351902704512067No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT3270.10134946659806723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1950.024.6666681
TTACTCG400.001928193723.12519
TAGTACC400.001928193723.1254
TAGGTAC855.15156E-821.7647063
GTCAACC1059.749783E-1021.14285715
ACGTTTA450.003819933620.5555571
AAGGGTC450.003819933620.5555575
TTGGCGT555.132079E-420.18181823
TACTAGC555.132079E-420.1818187
GTCTTAC555.132079E-420.18181817
GTATCAA5000.019.981
TTGACCG656.882478E-519.92307728
AACGATT656.882478E-519.92307722
TCAACCC1303.274181E-1119.92307716
GTTAGGT759.231939E-619.7333321
ATAGACA759.231939E-619.7333324
CAACCCA1355.4569682E-1119.18518617
ACCGCGA609.2171977E-418.531
ATTACCC1306.89397E-1018.53
TAACGGC1103.8260623E-818.536
Produced by FastQC (version 0.11.5)