FastQCFastQC Report
Fri 10 Feb 2017
ERR1632847.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632847.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453839
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39980.8809291400694961No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30730.6771123680424115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14220.3133269727810964No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC12520.27586875521936194No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC11100.24458012643250138No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG10980.24193601695755543No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG10550.23246129133899907No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA10540.2322409488827536No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT10390.22893581203907112No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG9780.21549492220809585No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC9640.21241012782065885No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC9290.2046981418520665No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9070.19985060781466554No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG8840.19478273132101914No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT8350.1839859509649898No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG8170.18001978675257085No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA8120.17891807447134336No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG8120.17891807447134336No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT7970.17561293762766092No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC7880.17362985552145144No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7840.17274848569646944No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC7790.17164677341524198No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7570.16679923937784102No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG7570.16679923937784102No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA7540.16613821200910456No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT7530.16591786955285906No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC6810.15005321270318328No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT6780.14939218533444681No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG6530.14388362392830936No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC6520.1436632814720639No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA6460.1423412267345909No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA6290.13859540497841746No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG6290.13859540497841746No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6150.1355106105909805No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG6000.13220547374729805No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG5820.1282393095348791No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT5740.12647656988491515No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT5590.1231714330412327No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT5570.1227307481287417No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5440.11986629619755024No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA5310.11700184426635878No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG5110.11259499514144884No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC5050.11127294040397587No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG5010.11039157057899386No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT4980.10973054321025738No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT4970.10951020075401188No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC4950.10906951584152089No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG4900.10796780356029341No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA4790.10554403654159294No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC4780.10532369408534745No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4750.10466266671661097No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG4730.10422198180411997No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT4720.10400163934787447No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT4650.10245924215415599No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC4650.10245924215415599No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA4580.10091684496043751No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG4550.10025581759170103No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGGT200.001840762437.010
GAGTTTA200.001840762437.021
ACCGTGT200.001840762437.08
GGAAGGA303.5960763E-430.83333421
GGGTCGT250.00549288229.67
TTTAAGT250.00549288229.624
CCGTGTC250.00549288229.69
GAATCGA250.00549288229.626
CGCGAAA250.00549288229.612
GTTGTAT250.00549288229.61
GTCGTGG250.00549288229.69
CGTGTCT250.00549288229.610
TCGAACC250.00549288229.629
TCGCCGT604.310641E-827.75000225
CGCCGTC556.247719E-726.9090926
GCCGTCC556.247719E-726.9090927
GGACCGT509.075266E-625.9000026
GGTATCA6900.025.4710161
GTCGCCG601.3344288E-624.66666824
AGTCGCC601.3344288E-624.66666823
Produced by FastQC (version 0.11.5)