FastQCFastQC Report
Fri 10 Feb 2017
ERR1632846.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632846.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67085
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1460.2176343444883357No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.1490646195125587No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC850.1267049265856749No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT790.11776104941492137No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC750.11179846463441902No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG700.10434523365879109No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC700.10434523365879109No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT200.001829603637.024
ATCAAGC251.2212698E-437.030
AGGCCAT200.001829603637.025
GGTATCA603.274181E-1133.9166681
CAAGCAG303.5637646E-430.83333432
AAGTTGA250.00545996429.629
CCATCAA358.782223E-426.42857228
CATCAGA358.782223E-426.42857216
CATCAAG451.3051493E-424.66666629
TTAGCCA400.001912386923.1254
GCCATCA400.001912386923.12527
AAAGTTG400.001912386923.12528
TCAAGCA400.001912386923.12531
CTTAGCC400.001912386923.1253
CAGATCA652.6208509E-622.7692336
AGATCAC704.9847586E-621.14285937
AGAGGCC450.003788918520.55555523
GCAGATC450.003788918520.55555535
GGCCATC555.0740165E-420.18181826
TCCAGGA656.7790876E-519.9230772
Produced by FastQC (version 0.11.5)