##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632846.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	67085
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.424774539762986	33.0	27.0	33.0	14.0	33.0
2	29.659253186256244	33.0	33.0	33.0	14.0	33.0
3	30.15726317358575	33.0	33.0	33.0	14.0	33.0
4	30.30494149213684	33.0	33.0	33.0	14.0	33.0
5	30.454557650741595	33.0	33.0	33.0	14.0	33.0
6	31.43145263471715	37.0	27.0	37.0	14.0	37.0
7	31.816590892151748	37.0	27.0	37.0	14.0	37.0
8	31.94565103972572	37.0	27.0	37.0	14.0	37.0
9	31.72026533502273	37.0	27.0	37.0	14.0	37.0
10	31.780785570544833	37.0	27.0	37.0	14.0	37.0
11	31.637534471193263	37.0	27.0	37.0	14.0	37.0
12	32.050294402623535	37.0	27.0	37.0	14.0	37.0
13	32.14226727286279	37.0	27.0	37.0	14.0	37.0
14	32.140523216814486	37.0	27.0	37.0	14.0	37.0
15	32.25212789744354	37.0	27.0	37.0	14.0	37.0
16	32.26722814340016	37.0	33.0	37.0	14.0	37.0
17	32.19864351196244	37.0	27.0	37.0	14.0	37.0
18	32.22393977789372	37.0	27.0	37.0	14.0	37.0
19	32.2196019974659	37.0	27.0	37.0	14.0	37.0
20	32.15220988298427	37.0	27.0	37.0	14.0	37.0
21	32.19909070582097	37.0	27.0	37.0	14.0	37.0
22	32.17904151449653	37.0	27.0	37.0	14.0	37.0
23	32.15814265484087	37.0	27.0	37.0	14.0	37.0
24	32.20414399642245	37.0	27.0	37.0	14.0	37.0
25	32.173272713721396	37.0	27.0	37.0	14.0	37.0
26	32.042766639338154	37.0	27.0	37.0	14.0	37.0
27	31.969441752999927	37.0	27.0	37.0	14.0	37.0
28	31.963941268539912	37.0	27.0	37.0	14.0	37.0
29	31.96508906611016	37.0	27.0	37.0	14.0	37.0
30	31.895162853096817	37.0	27.0	37.0	14.0	37.0
31	31.905686815234404	37.0	27.0	37.0	14.0	37.0
32	31.913885369307597	37.0	27.0	37.0	14.0	37.0
33	31.8462994708206	37.0	27.0	37.0	14.0	37.0
34	31.751509279272565	37.0	27.0	37.0	14.0	37.0
35	31.771424312439443	37.0	27.0	37.0	14.0	37.0
36	31.711351270775882	37.0	27.0	37.0	14.0	37.0
37	31.792263546247298	37.0	27.0	37.0	14.0	37.0
38	31.676887530744576	37.0	27.0	37.0	14.0	37.0
39	31.7270328687486	37.0	27.0	37.0	14.0	37.0
40	31.756055750167697	37.0	27.0	37.0	14.0	37.0
41	31.760706566296488	37.0	27.0	37.0	14.0	37.0
42	31.6884102258329	37.0	27.0	37.0	14.0	37.0
43	31.32677945889543	37.0	27.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	3.0
16	12.0
17	12.0
18	12.0
19	69.0
20	123.0
21	314.0
22	558.0
23	929.0
24	1504.0
25	2000.0
26	2606.0
27	3331.0
28	3941.0
29	4521.0
30	5233.0
31	5984.0
32	6676.0
33	7049.0
34	7715.0
35	8313.0
36	6179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.46083327122307	24.108220913766118	10.257136468659164	24.173809346351643
2	17.394350450920474	26.00581352016099	33.348736677349635	23.251099351568904
3	19.481255124096293	28.85891033763136	28.912573600655882	22.747260937616456
4	15.580234031452637	22.331370649176417	35.89177908623388	26.196616233137064
5	16.766788402772605	37.418200790042484	34.003130357009766	11.81188045017515
6	29.365730043974065	40.61563687858687	17.361556234627713	12.65707684281136
7	26.365059253186256	33.090854885592904	23.702765148691956	16.84132071252888
8	24.422747260937616	36.87113363643139	20.44570321234255	18.26041589028844
9	25.697249757769992	16.201833494820004	20.5127822911232	37.588134456286795
10	15.034657524036671	29.152567638071105	33.70052917939927	22.112245658492956
11	33.582768129984345	23.827979429082507	24.55392412610867	18.035328314824476
12	22.733845121860327	25.943206379965716	30.61340090929418	20.70954758887978
13	26.937467392114485	22.95446075873891	26.35760602221063	23.750465826935976
14	21.353506745174034	21.334128344637403	27.845270924945964	29.467093985242602
15	23.477677573227997	29.99925467690244	24.282626518595812	22.24044123127376
16	22.754714168592084	28.87381679958262	25.127822911232016	23.24364612059328
17	21.717224416784674	29.024372065290304	26.871878959528956	22.386524558396065
18	21.96616233137065	28.568234329581877	27.85570544831184	21.60989789073563
19	23.73257807259447	27.599314302750244	27.40702094357904	21.261086681076247
20	23.14824476410524	27.550122978311098	27.65148691957964	21.650145338004027
21	22.65782216590892	28.02563911455616	27.562048147872105	21.754490571662817
22	22.619065364835656	27.734963106506672	27.13423268987106	22.511738838786613
23	22.474472683908473	27.438324513676676	27.86315867928747	22.224044123127378
24	22.40143102034732	28.33569352314228	26.420213162405904	22.842662294104493
25	23.061787284787954	27.72601922933592	27.50093165387195	21.711261832004176
26	22.639934411567413	28.064395915629426	27.90042483416561	21.395244838637552
27	23.20488931952001	27.59484236416487	26.634866214503987	22.565402101811134
28	22.328389356786165	27.469628083774317	28.04352686889767	22.15845569054185
29	23.40761720205709	27.37720801967653	26.98218677796825	22.23298800029813
30	23.151226056495492	27.25199373928598	27.526272639189088	22.07050756502944
31	22.7323544756652	27.52925393157934	27.56652008645748	22.17187150629798
32	21.824550942833717	27.368264142505776	27.976447790117014	22.83073712454349
33	22.37161809644481	27.94812551240963	27.82589252440933	21.85436386673623
34	22.832227770738616	27.861668033092347	27.4278899903108	21.87821420585824
35	22.70850413654319	28.042036222702542	27.365282850115523	21.884176790638744
36	23.491093388984126	27.93172840426325	26.49623611835731	22.080942088395318
37	22.923157188641277	27.257956324066484	27.23112469255422	22.587761794738018
38	23.251099351568904	27.480062607140194	27.06417231870016	22.204665722590743
39	22.34329581873742	27.59484236416487	27.60229559514049	22.45956622195722
40	22.507266900201238	28.10613400909294	27.839308340165463	21.54729075054036
41	22.26280092420064	27.715584705970038	27.781173138555566	22.24044123127376
42	22.253857047029886	27.743906983677423	27.69769695162853	22.30453901766416
43	23.31668778415443	26.499217410747562	27.9347096966535	22.24938510844451
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	5.5
4	11.0
5	11.0
6	17.0
7	23.0
8	26.5
9	30.0
10	39.5
11	49.0
12	49.0
13	80.5
14	112.0
15	186.0
16	260.0
17	306.5
18	353.0
19	353.0
20	362.5
21	372.0
22	469.5
23	567.0
24	747.0
25	927.0
26	927.0
27	1225.5
28	1524.0
29	1840.5
30	2157.0
31	2611.5
32	3066.0
33	3066.0
34	3567.5
35	4069.0
36	4421.0
37	4773.0
38	5020.0
39	5267.0
40	5267.0
41	5513.0
42	5759.0
43	5828.0
44	5897.0
45	5580.5
46	5264.0
47	5264.0
48	4940.0
49	4616.0
50	4501.0
51	4386.0
52	4221.5
53	4057.0
54	4057.0
55	3866.5
56	3676.0
57	3263.5
58	2851.0
59	2499.0
60	2147.0
61	2147.0
62	1948.5
63	1750.0
64	1376.5
65	1003.0
66	807.0
67	611.0
68	611.0
69	532.0
70	453.0
71	382.5
72	312.0
73	308.0
74	304.0
75	304.0
76	272.5
77	241.0
78	178.5
79	116.0
80	74.0
81	32.0
82	32.0
83	26.5
84	21.0
85	19.5
86	18.0
87	12.5
88	7.0
89	7.0
90	4.5
91	2.0
92	1.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	67085.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.17395841097115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.38220544209904	72.21882686144444
2	3.832732681196728	5.992397704404859
3	1.2451614133440116	2.920175896251025
4	0.6292546193009553	1.9676529775657747
5	0.43094407261216944	1.6844302004919134
6	0.27649066605648037	1.2968621897592605
7	0.21547203630608472	1.1791011403443392
8	0.16398756745418835	1.025564582246404
9	0.1449192456571897	1.0196019974659014
>10	0.6502297732776539	9.09294179026608
>50	0.026695650515798103	1.3848103152716702
>100	0.0019068321796998646	0.2176343444883357
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	146	0.2176343444883357	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	100	0.1490646195125587	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	85	0.1267049265856749	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	79	0.11776104941492137	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	75	0.11179846463441902	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	70	0.10434523365879109	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	70	0.10434523365879109	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.001490646195125587	0.0	0.0
6	0.0	0.0	0.002981292390251174	0.0	0.0
7	0.0	0.0	0.002981292390251174	0.0	0.0
8	0.0	0.0	0.002981292390251174	0.0	0.0
9	0.0	0.0	0.002981292390251174	0.0	0.0
10	0.001490646195125587	0.0	0.002981292390251174	0.0	0.0
11	0.001490646195125587	0.0	0.002981292390251174	0.0	0.0
12	0.001490646195125587	0.0	0.002981292390251174	0.0	0.0
13	0.001490646195125587	0.0	0.002981292390251174	0.0	0.0
14	0.001490646195125587	0.0	0.002981292390251174	0.0	0.0
15	0.001490646195125587	0.0	0.002981292390251174	0.0	0.0
16	0.001490646195125587	0.0	0.002981292390251174	0.0	0.0
17	0.001490646195125587	0.0	0.002981292390251174	0.0	0.0
18	0.001490646195125587	0.0	0.002981292390251174	0.0	0.0
19	0.001490646195125587	0.0	0.002981292390251174	0.0	0.0
20	0.001490646195125587	0.0	0.002981292390251174	0.0	0.0
21	0.001490646195125587	0.0	0.002981292390251174	0.0	0.0
22	0.001490646195125587	0.0	0.002981292390251174	0.0	0.0
23	0.001490646195125587	0.0	0.002981292390251174	0.0	0.0
24	0.001490646195125587	0.0	0.002981292390251174	0.0	0.0
25	0.001490646195125587	0.0	0.002981292390251174	0.0	0.0
26	0.001490646195125587	0.0	0.002981292390251174	0.0	0.0
27	0.001490646195125587	0.0	0.004471938585376761	0.0	0.0
28	0.001490646195125587	0.0	0.004471938585376761	0.002981292390251174	0.0
29	0.001490646195125587	0.0	0.004471938585376761	0.004471938585376761	0.0
30	0.001490646195125587	0.0	0.004471938585376761	0.013415815756130283	0.0
31	0.001490646195125587	0.0	0.004471938585376761	0.03875680107326526	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAT	20	0.0018296036	37.0	24
ATCAAGC	25	1.2212698E-4	37.0	30
AGGCCAT	20	0.0018296036	37.0	25
GGTATCA	60	3.274181E-11	33.916668	1
CAAGCAG	30	3.5637646E-4	30.833334	32
AAGTTGA	25	0.005459964	29.6	29
CCATCAA	35	8.782223E-4	26.428572	28
CATCAGA	35	8.782223E-4	26.428572	16
CATCAAG	45	1.3051493E-4	24.666666	29
TTAGCCA	40	0.0019123869	23.125	4
GCCATCA	40	0.0019123869	23.125	27
AAAGTTG	40	0.0019123869	23.125	28
TCAAGCA	40	0.0019123869	23.125	31
CTTAGCC	40	0.0019123869	23.125	3
CAGATCA	65	2.6208509E-6	22.76923	36
AGATCAC	70	4.9847586E-6	21.142859	37
AGAGGCC	45	0.0037889185	20.555555	23
GCAGATC	45	0.0037889185	20.555555	35
GGCCATC	55	5.0740165E-4	20.181818	26
TCCAGGA	65	6.7790876E-5	19.923077	2
>>END_MODULE