FastQCFastQC Report
Fri 10 Feb 2017
ERR1632845.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632845.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352752
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8500.24096248922755933No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5860.16612237492629384No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGC250.00549124329.619
GGTATCA1750.028.5428561
CTAGACT451.320674E-424.6666664
GGTACCG400.001928549423.1256
GCCGTTA400.001928549423.12515
ACAACCG502.69696E-422.213
GTATCAA5000.022.1999991
GTCTAAT603.716869E-521.5833341
CTAAGGC450.003820632620.5555553
CCGTTAA450.003820632620.55555516
GCGAACC555.1333866E-420.18181830
GGCGAAC555.1333866E-420.18181829
AAGATAG851.240307E-619.5882365
AACCGGG609.219525E-418.533
CGAACCG609.219525E-418.531
TAGACTG500.007025453718.55
TGGACTG500.007025453718.55
CGGCCGT500.007025453718.513
ACCGCGG500.007025453718.59
GACGTCT500.007025453718.515
Produced by FastQC (version 0.11.5)