FastQCFastQC Report
Fri 10 Feb 2017
ERR1632844.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632844.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320523
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6550.20435350973253089No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5250.16379479787721943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC509.064403E-625.9000027
GTTACTA451.3203052E-424.6666689
CGAGCGT451.3203052E-424.6666686
GCTACGA451.3203052E-424.6666682
GGTATCA1300.024.1923081
TGAACGC400.001928165823.12500225
TTAGTCG400.001928165823.12500214
AATACCC400.001928165823.1250025
TGTAGGA903.801688E-922.6111132
GTATTCC502.696214E-422.21
CGATGGG502.696214E-422.219
TAGGACA502.696214E-422.24
ACGAGCG502.696214E-422.25
ATAATAC705.083004E-621.1428573
CGTGCTT450.003819879620.55555719
GTCACTA450.003819879620.55555732
AGTCGTG450.003819879620.55555716
GTATCAA3200.020.2343751
CTTAAGG555.13198E-420.1818183
TACGGGT555.13198E-420.18181814
Produced by FastQC (version 0.11.5)