##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632844.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	320523
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.658526845187396	33.0	33.0	33.0	27.0	33.0
2	31.200881060017533	33.0	33.0	33.0	27.0	33.0
3	31.64194769174131	33.0	33.0	33.0	27.0	33.0
4	31.77938244681349	33.0	33.0	33.0	27.0	33.0
5	31.88046411645966	33.0	33.0	33.0	33.0	33.0
6	34.738293351803144	37.0	37.0	37.0	27.0	37.0
7	34.902325262149674	37.0	37.0	37.0	27.0	37.0
8	35.09170012760395	37.0	37.0	37.0	33.0	37.0
9	35.17136991729143	37.0	37.0	37.0	33.0	37.0
10	35.240659796644856	37.0	37.0	37.0	33.0	37.0
11	35.237636612661184	37.0	37.0	37.0	33.0	37.0
12	35.18425822795868	37.0	37.0	37.0	33.0	37.0
13	35.21645248546906	37.0	37.0	37.0	33.0	37.0
14	35.19521843986235	37.0	37.0	37.0	33.0	37.0
15	35.220224445671604	37.0	37.0	37.0	33.0	37.0
16	35.20641888413624	37.0	37.0	37.0	33.0	37.0
17	35.16604424643473	37.0	37.0	37.0	33.0	37.0
18	35.20101209585584	37.0	37.0	37.0	33.0	37.0
19	35.20762940569008	37.0	37.0	37.0	33.0	37.0
20	35.21323274772793	37.0	37.0	37.0	33.0	37.0
21	35.18206181771667	37.0	37.0	37.0	33.0	37.0
22	35.189340546544244	37.0	37.0	37.0	33.0	37.0
23	35.18550930822437	37.0	37.0	37.0	33.0	37.0
24	35.191739750345526	37.0	37.0	37.0	33.0	37.0
25	35.190270277016005	37.0	37.0	37.0	33.0	37.0
26	35.12519850369552	37.0	37.0	37.0	33.0	37.0
27	35.06801695978136	37.0	37.0	37.0	33.0	37.0
28	35.05645148710077	37.0	37.0	37.0	27.0	37.0
29	35.090932631979605	37.0	37.0	37.0	33.0	37.0
30	35.05064535150363	37.0	37.0	37.0	27.0	37.0
31	35.04349453861345	37.0	37.0	37.0	27.0	37.0
32	35.08200659547053	37.0	37.0	37.0	33.0	37.0
33	35.044851695510154	37.0	37.0	37.0	27.0	37.0
34	34.98913338512369	37.0	37.0	37.0	27.0	37.0
35	34.996380914942144	37.0	37.0	37.0	27.0	37.0
36	34.96525990334547	37.0	37.0	37.0	27.0	37.0
37	34.986025963815386	37.0	37.0	37.0	27.0	37.0
38	34.85966373707971	37.0	37.0	37.0	27.0	37.0
39	34.93793269125773	37.0	37.0	37.0	27.0	37.0
40	34.97578956892329	37.0	37.0	37.0	27.0	37.0
41	34.98850004523856	37.0	37.0	37.0	27.0	37.0
42	34.90909856702951	37.0	37.0	37.0	27.0	37.0
43	34.6890301164035	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	6.0
16	16.0
17	8.0
18	12.0
19	21.0
20	18.0
21	89.0
22	207.0
23	485.0
24	915.0
25	1690.0
26	2806.0
27	4134.0
28	5914.0
29	7742.0
30	10341.0
31	12787.0
32	16274.0
33	21284.0
34	29940.0
35	53675.0
36	152159.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.50348960917001	20.190438751665248	13.110759602275031	26.195312036889707
2	16.030674865766265	23.07073127357475	36.899068085597605	23.999525775061382
3	17.865488592082315	25.551988468846233	30.221856153848552	26.360666785222904
4	13.000627100083301	17.633679954324652	38.39911644406174	30.966576501530312
5	14.736851957581829	36.106613253963054	34.9023315019515	14.25420328650362
6	30.85706797952097	38.45745859111515	17.636487865145405	13.04898556421848
7	27.448576233218837	31.6270595245895	23.170568102757056	17.75379613943461
8	24.981358592051116	35.5035364076837	21.12328912433741	18.39181587592778
9	26.473919188326576	15.111240067015471	19.940222698527094	38.47461804613086
10	14.469164459336772	28.017646159557973	34.5310632934298	22.982126087675457
11	34.61779653878193	22.8264430321693	24.479054545227644	18.076705883821127
12	22.713190629065622	24.774509161589027	31.374347550721787	21.13795265862356
13	28.386730437441305	21.39721642440636	25.972239121685497	24.243814016466835
14	21.9076322136009	20.361409321639947	27.864459024781375	29.866499439977783
15	23.769589077850885	28.720871825110834	23.46664669930083	24.042892397737447
16	22.98711792913457	28.297189281268427	25.04594054092842	23.669752248668583
17	23.164952281115553	27.643882030306717	26.390305843886395	22.80085984469133
18	23.13624919272564	27.21146376391086	27.359035077045952	22.293251966317552
19	24.224470630812768	26.670472945779245	26.80774858590491	22.29730783750308
20	23.903432826973415	26.813676397637607	26.679832648515085	22.60305812687389
21	23.501589589514637	26.605579006810743	26.87857033660611	23.01426106706851
22	23.217054626345067	26.80369271471938	26.93691248365952	23.04234017527603
23	23.235462041725555	26.32697185537387	27.05110085703678	23.386465245863793
24	23.41423236398012	26.567204225593795	26.58155576978875	23.437007640637333
25	23.61328204216234	26.116066553726252	27.440152500756575	22.83049890335483
26	23.91716039098598	26.389681863704006	26.369090517685155	23.32406722762485
27	23.40612062160906	27.225191327923426	26.535381236291933	22.833306814175582
28	23.0442121158232	26.918817058370227	26.64052189702455	23.39644892878202
29	23.217366616436262	26.89635377180421	26.878258346514915	23.008021265244615
30	23.376169572854366	26.674840807055965	26.91975302864381	23.029236591445855
31	23.649160902649733	26.27174960923241	26.838635604933188	23.24045388318467
32	23.29817205005569	26.634906075383046	26.784037338974116	23.28288453558715
33	23.118465757527538	26.647697669122028	27.240166852300774	22.99366972104966
34	23.39301703777888	26.090171376157095	26.59341139325415	23.923400192809876
35	22.97900618676351	26.625546372647207	26.931920642200403	23.463526798388884
36	23.75648549402071	26.19874392789285	26.614938709546585	23.429831868539853
37	23.711558920888674	26.596219304074904	26.668601005232073	23.02362076980435
38	23.516253123800787	26.62180249155287	26.87170655459983	22.990237830046517
39	23.180239795584093	26.504182227172464	27.022397768646865	23.293180208596574
40	23.17088009284825	26.23649472892741	27.218951526099534	23.373673652124808
41	23.351834345741178	26.077067792326915	27.081987876065057	23.48910998586685
42	23.22859825971927	26.797452912895487	26.879818296970885	23.094130530414354
43	23.35121036555879	26.20560770989913	27.177768834061833	23.265413090480248
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	7.5
2	13.0
3	51.0
4	89.0
5	89.0
6	112.5
7	136.0
8	126.0
9	116.0
10	161.5
11	207.0
12	207.0
13	320.5
14	434.0
15	757.5
16	1081.0
17	1228.0
18	1375.0
19	1375.0
20	1452.0
21	1529.0
22	1794.0
23	2059.0
24	2898.5
25	3738.0
26	3738.0
27	4902.0
28	6066.0
29	7649.5
30	9233.0
31	10691.5
32	12150.0
33	12150.0
34	14163.0
35	16176.0
36	17425.0
37	18674.0
38	20249.5
39	21825.0
40	21825.0
41	22872.0
42	23919.0
43	24503.5
44	25088.0
45	25516.5
46	25945.0
47	25945.0
48	25855.5
49	25766.0
50	25452.5
51	25139.0
52	24258.0
53	23377.0
54	23377.0
55	21297.5
56	19218.0
57	17141.5
58	15065.0
59	13983.5
60	12902.0
61	12902.0
62	11546.5
63	10191.0
64	8339.0
65	6487.0
66	5646.0
67	4805.0
68	4805.0
69	3881.0
70	2957.0
71	2494.0
72	2031.0
73	1592.0
74	1153.0
75	1153.0
76	915.5
77	678.0
78	537.5
79	397.0
80	326.5
81	256.0
82	256.0
83	181.0
84	106.0
85	96.0
86	86.0
87	59.5
88	33.0
89	33.0
90	26.0
91	19.0
92	10.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	320523.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.03156355287832
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.36848937289571	63.47983532515572
2	6.134373448833091	8.714682749757541
3	1.6072296942022846	3.424921145034083
4	0.741815895470413	2.107693716945679
5	0.43469371875914564	1.5438487255038635
6	0.30349441539627403	1.2934609713098455
7	0.21980549996067186	1.092918983781006
8	0.1473498187750106	0.8373190413457816
9	0.12409106792589332	0.7932944319950367
>10	0.8341901543251066	11.492498305486372
>50	0.06589303719898708	3.0954029340826703
>100	0.017689405959454667	1.7534543513340388
>500	8.844702979727333E-4	0.3706693182683923
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	655	0.20435350973253089	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	525	0.16379479787721943	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.1199009119470364E-4	0.0	0.0	0.0	0.0
3	3.1199009119470364E-4	0.0	0.0	0.0	0.0
4	3.1199009119470364E-4	0.0	3.1199009119470364E-4	0.0	0.0
5	3.1199009119470364E-4	0.0	3.1199009119470364E-4	0.0	0.0
6	3.1199009119470364E-4	0.0	9.359702735841109E-4	0.0	0.0
7	3.1199009119470364E-4	0.0	0.002807910820752333	0.0	0.0
8	3.1199009119470364E-4	0.0	0.00343189100314174	0.0	0.0
9	6.239801823894073E-4	0.0	0.004991841459115258	6.239801823894073E-4	0.0
10	9.359702735841109E-4	0.0	0.005303831550309962	9.359702735841109E-4	0.0
11	9.359702735841109E-4	0.0	0.007799752279867591	9.359702735841109E-4	0.0
12	9.359702735841109E-4	0.0	0.008735722553451702	9.359702735841109E-4	0.0
13	9.359702735841109E-4	0.0	0.009047712644646407	9.359702735841109E-4	0.0
14	9.359702735841109E-4	0.0	0.009047712644646407	0.0012479603647788146	0.0
15	9.359702735841109E-4	0.0	0.009983682918230517	0.0012479603647788146	0.0
16	9.359702735841109E-4	0.0	0.011543633374204035	0.0012479603647788146	0.0
17	9.359702735841109E-4	0.0	0.012167613556593442	0.0012479603647788146	0.0
18	9.359702735841109E-4	3.1199009119470364E-4	0.012479603647788147	0.0012479603647788146	0.0
19	9.359702735841109E-4	3.1199009119470364E-4	0.01279159373898285	0.0012479603647788146	0.0
20	9.359702735841109E-4	3.1199009119470364E-4	0.013103583830177554	0.0015599504559735183	0.0
21	9.359702735841109E-4	3.1199009119470364E-4	0.013415573921372258	0.0015599504559735183	0.0
22	9.359702735841109E-4	3.1199009119470364E-4	0.014039554103761665	0.0015599504559735183	0.0
23	9.359702735841109E-4	3.1199009119470364E-4	0.014039554103761665	0.0018719405471682219	0.0
24	9.359702735841109E-4	3.1199009119470364E-4	0.014039554103761665	0.002495920729557629	0.0
25	9.359702735841109E-4	3.1199009119470364E-4	0.014039554103761665	0.002807910820752333	0.0
26	0.0012479603647788146	3.1199009119470364E-4	0.014039554103761665	0.00343189100314174	0.0
27	0.0012479603647788146	3.1199009119470364E-4	0.014039554103761665	0.0037438810943364437	0.0
28	0.0012479603647788146	3.1199009119470364E-4	0.014039554103761665	0.009047712644646407	0.0
29	0.0012479603647788146	3.1199009119470364E-4	0.014039554103761665	0.020903336110045145	0.0
30	0.0012479603647788146	3.1199009119470364E-4	0.014039554103761665	0.04024672176411677	0.0
31	0.0012479603647788146	3.1199009119470364E-4	0.014039554103761665	0.10077279945588928	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCGTC	50	9.064403E-6	25.900002	7
GTTACTA	45	1.3203052E-4	24.666668	9
CGAGCGT	45	1.3203052E-4	24.666668	6
GCTACGA	45	1.3203052E-4	24.666668	2
GGTATCA	130	0.0	24.192308	1
TGAACGC	40	0.0019281658	23.125002	25
TTAGTCG	40	0.0019281658	23.125002	14
AATACCC	40	0.0019281658	23.125002	5
TGTAGGA	90	3.801688E-9	22.611113	2
GTATTCC	50	2.696214E-4	22.2	1
CGATGGG	50	2.696214E-4	22.2	19
TAGGACA	50	2.696214E-4	22.2	4
ACGAGCG	50	2.696214E-4	22.2	5
ATAATAC	70	5.083004E-6	21.142857	3
CGTGCTT	45	0.0038198796	20.555557	19
GTCACTA	45	0.0038198796	20.555557	32
AGTCGTG	45	0.0038198796	20.555557	16
GTATCAA	320	0.0	20.234375	1
CTTAAGG	55	5.13198E-4	20.181818	3
TACGGGT	55	5.13198E-4	20.181818	14
>>END_MODULE