FastQCFastQC Report
Fri 10 Feb 2017
ERR1632843.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632843.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259401
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12920.4980705548552241No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10590.40824823343009475No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6590.25404682325819866No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6530.25173380210562024No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC6440.24826427037675258No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT6330.24402373159702542No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG5750.22166452712210055No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA5470.2108704284100678No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC5420.20894291078291913No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC5270.20316035790147302No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC5110.19699230149459718No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5060.19506478386744847No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG4950.19082424508772133No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA4830.18619820278256444No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG4580.17656061464682093No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG4530.17463309701967222No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT4490.17309108291795328No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC4430.17077806176537483No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT4360.16807953708736664No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC4310.166152019460218No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG4200.16191148068049083No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT4130.15921295600248264No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT4030.15535792074818525No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG4000.15420141017189604No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT3990.1538159066464663No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT3990.1538159066464663No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC3960.15265939607017706No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG3950.15227389254474732No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA3890.14996087139216888No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC3730.14379281498529303No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG3580.13801026210384693No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA3550.13685375152755772No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT3450.13299871627326032No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG3330.12837267396810342No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC3240.12490314223923576No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA3220.12413213518837629No Hit
ACCTTAGCCAAACCATTTACCCAGATAAAGTATAGGCGATAGA3200.1233611281375168No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA3160.12181911403579784No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG3140.12104810698493837No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA3130.12066260345950863No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3120.12027709993407888No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT3110.11989159640864917No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT3100.11950609288321942No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA3070.11834958230693021No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG3060.11796407878150046No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTC3010.11603656115435176No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT3000.11565105762892203No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT2960.11410904352720307No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA2930.11295253295091384No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGC2930.11295253295091384No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG2870.11063951179833541No Hit
GATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGAT2860.11025400827290566No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC2850.10986850474747592No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG2840.10948300122204618No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTAC2780.10716998006946774No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC2770.106784476544038No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT2670.1029294412897406No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGA2600.10023091661173242No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGATA200.001839309837.02
GCTCTAG200.001839309837.01
ATCTATA250.005488597329.623
TCTCTGC250.005488597329.621
GTCTCTG250.005488597329.620
GCCTACC250.005488597329.68
TAGATGG250.005488597329.67
TGCCGAA250.005488597329.631
TGTTTAC358.8508136E-426.42857214
TTTCGCC358.8508136E-426.42857229
ATAGATG358.8508136E-426.4285726
CCTGTTT358.8508136E-426.42857212
CTGTTTA358.8508136E-426.42857213
GGTATCA2550.026.1176471
TTTACAC451.3193568E-424.6666683
ACCAACC601.3307472E-624.6666668
GCCGAGT551.8953053E-523.54545412
AGTTCCT551.8953053E-523.54545416
GAGTTCC551.8953053E-523.54545415
TATTTCG400.001927179923.12500227
Produced by FastQC (version 0.11.5)