FastQCFastQC Report
Fri 10 Feb 2017
ERR1632842.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632842.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265897
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8470.31854439877095264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6820.25649029511427357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3070.11545824134909383No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAC250.0054888429.635
CCGGTGC250.0054888429.633
CCCCGTC358.851398E-426.428577
GGTATCA1650.023.5454541
AGCCGGT400.001927305423.12500231
AGTTAGC400.001927305423.12500227
CGGAGTT400.001927305423.12500224
ATAAAGG855.1430106E-821.7647063
ATTATAG603.7124344E-521.5833343
GTATCAA4300.021.0813941
CACGGAG450.003818192520.55555522
GTTAGCC450.003818192520.55555528
CCCGTCT500.007021012318.58
GGAGTTA500.007021012318.525
GAGTTAG500.007021012318.526
CCACGTG500.007021012318.520
ATATTAT701.2147296E-418.51
TCTATAC500.007021012318.53
CCGCGGC752.0597455E-417.2666669
TAAACTG650.001575618717.0769235
Produced by FastQC (version 0.11.5)