FastQCFastQC Report
Fri 10 Feb 2017
ERR1632841.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632841.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349841
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9260.26469167421771606No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG7330.20952375507730653No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6810.1946598597648646No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA6280.17951012031179878No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG5380.1537841476556493No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC4450.12720064257762811No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT4230.12091207148390269No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC4210.12034038320265493No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT4180.11948285078078327No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA4110.11748194179641608No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG3980.1137659679683056No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC3970.11348012382768173No Hit
GATACGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG3790.10833492929645183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3700.10576233203083688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTTA358.8570156E-426.4285725
TAGGACC659.35288E-825.6153854
GGTATCA2450.023.4081651
AGCGTTC400.001928517223.12537
AAAGCGT502.6968986E-422.19999935
AACTGTG806.9316775E-720.81257
AACGTCA450.003820568320.55555728
GCGGGTA450.003820568320.55555722
TTAGGAC1001.2820237E-820.3499983
TAGGGGT656.8846006E-519.9230774
GTATCAA5400.019.870371
GATAAGA609.219317E-418.52
CGCCCTA609.219317E-418.516
GTATTGG500.00702534218.4999981
GTATTAA500.00702534218.4999981
TGCGGGT500.00702534218.49999821
GAAAGCG500.00702534218.49999834
CGTCAAT500.00702534218.49999830
TGCGTTT500.00702534218.49999824
TTATACT1351.1423253E-917.8148164
Produced by FastQC (version 0.11.5)