FastQCFastQC Report
Fri 10 Feb 2017
ERR1632840.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632840.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188258
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16300.8658330588872717No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14350.7622518033762177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6910.3670494746571195No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5280.2804661687683923No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4090.21725504361036452No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.19175811917687427No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3260.17316661177745435No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3030.16094933548640697No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2950.15669984808082527No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT2460.13067173772163734No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2430.12907817994454418No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2350.12482869253896249No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG2270.12057920513338079No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2230.11845446143058994No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2200.11686090365349679No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2190.11632971772779908No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC2180.11579853180210137No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2150.11420497402500823No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC2150.11420497402500823No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2140.11367378809931052No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT2110.1120802303222174No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC2110.1120802303222174No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2100.11154904439651966No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT2070.10995548661942654No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC2060.10942430069372881No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT2050.10889311476803111No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT2010.10676837106524026No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA1990.10570599921384484No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA1950.10358125551105399No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1940.10305006958535627No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG1930.10251888365965857No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC1890.10039413995686769No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTT200.001838029637.04
TACTACG200.001838029637.02
TACGTTG200.001838029637.05
CCGACGA250.0054848229.613
CGCCGTT250.0054848229.625
CCGCCGT453.984893E-628.77777924
GTCCGAC358.841756E-426.42857211
GTATCAA6750.026.311111
CCTTAGG400.001925226923.1251
TCTACAG400.001925226923.1255
TACTGTG400.001925226923.1257
ACATCGA400.001925226923.12520
CCTGCAT400.001925226923.12536
CTGACAA502.6904888E-422.232
GACAATT603.705023E-521.58333434
GGTATCA2000.021.2750021
ACAGTCC450.003814111720.5555578
GTCTCGT450.003814111720.55555734
ACCTGCA450.003814111720.55555735
TCGAGGT450.003814111720.55555723
Produced by FastQC (version 0.11.5)