##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632840.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	188258
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.574934398538176	33.0	33.0	33.0	27.0	33.0
2	30.373466200639548	33.0	33.0	33.0	27.0	33.0
3	30.85965005471215	33.0	33.0	33.0	27.0	33.0
4	31.012376632068758	33.0	33.0	33.0	27.0	33.0
5	31.182090535329177	33.0	33.0	33.0	27.0	33.0
6	33.52463640323386	37.0	33.0	37.0	27.0	37.0
7	33.77195125837946	37.0	33.0	37.0	27.0	37.0
8	33.95840283015861	37.0	33.0	37.0	27.0	37.0
9	33.9707635266496	37.0	33.0	37.0	27.0	37.0
10	33.98525959056189	37.0	33.0	37.0	27.0	37.0
11	33.91859044502757	37.0	33.0	37.0	27.0	37.0
12	33.990183684093104	37.0	33.0	37.0	27.0	37.0
13	34.056757216160804	37.0	33.0	37.0	27.0	37.0
14	34.034628010496235	37.0	33.0	37.0	27.0	37.0
15	34.07901921830679	37.0	33.0	37.0	27.0	37.0
16	34.09011038043536	37.0	33.0	37.0	27.0	37.0
17	34.0486088240606	37.0	33.0	37.0	27.0	37.0
18	34.06880451295562	37.0	33.0	37.0	27.0	37.0
19	34.054414686228476	37.0	33.0	37.0	27.0	37.0
20	34.055689532450145	37.0	33.0	37.0	27.0	37.0
21	34.041974311848634	37.0	33.0	37.0	27.0	37.0
22	34.027520742810395	37.0	33.0	37.0	27.0	37.0
23	34.005848357041934	37.0	33.0	37.0	27.0	37.0
24	33.9702323407239	37.0	33.0	37.0	27.0	37.0
25	33.95771760031446	37.0	33.0	37.0	27.0	37.0
26	33.9317479204071	37.0	33.0	37.0	27.0	37.0
27	33.83846635999532	37.0	33.0	37.0	27.0	37.0
28	33.79690106130948	37.0	33.0	37.0	27.0	37.0
29	33.80291939784763	37.0	33.0	37.0	27.0	37.0
30	33.77799084235464	37.0	33.0	37.0	27.0	37.0
31	33.74107873237791	37.0	33.0	37.0	27.0	37.0
32	33.7408450105706	37.0	33.0	37.0	27.0	37.0
33	33.69530112930128	37.0	33.0	37.0	27.0	37.0
34	33.58468697213399	37.0	33.0	37.0	27.0	37.0
35	33.5737711013609	37.0	33.0	37.0	27.0	37.0
36	33.54759957080177	37.0	33.0	37.0	27.0	37.0
37	33.55855793644891	37.0	33.0	37.0	27.0	37.0
38	33.41280583029672	37.0	33.0	37.0	22.0	37.0
39	33.49465626958748	37.0	33.0	37.0	27.0	37.0
40	33.4633800422824	37.0	33.0	37.0	22.0	37.0
41	33.48688501949452	37.0	33.0	37.0	22.0	37.0
42	33.305384100542874	37.0	33.0	37.0	14.0	37.0
43	32.9853073972952	37.0	33.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	11.0
16	12.0
17	13.0
18	22.0
19	22.0
20	66.0
21	202.0
22	452.0
23	892.0
24	1543.0
25	2501.0
26	3728.0
27	5094.0
28	6495.0
29	7975.0
30	9825.0
31	11896.0
32	14053.0
33	17555.0
34	23107.0
35	35436.0
36	47356.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.423472043684725	21.768530421017964	13.135696756578739	23.672300778718565
2	16.954392376419595	25.312602917273104	35.716941643914204	22.016063062393098
3	19.013269024423927	28.262278362672504	30.322217382528233	22.402235230375336
4	12.929065431482329	20.013492122512723	39.35768997864633	27.699752467358625
5	14.692602704798732	37.46135622390549	34.209967172709796	13.636073898585982
6	27.189282792763127	42.13207406856548	17.268323258506943	13.410319880164456
7	25.27382634469717	33.43337334933973	23.059843406389106	18.23295689957399
8	24.534415536125955	34.737434796927616	20.850641141412318	19.877508525534108
9	26.47749365232819	14.217722487224979	20.294489477206813	39.01029438324002
10	15.826684656163351	27.647165060714553	33.23046032572321	23.29568995739889
11	34.14250656014618	24.201893146639186	23.733387160173805	17.922213133040827
12	22.72413390134815	26.763271680353558	30.01094243006937	20.501651988228918
13	29.007532216426394	22.952543849398165	25.760392652636277	22.279531281539164
14	22.113801272721478	21.67875999957505	29.326774957770716	26.88066376993275
15	24.784604107129578	28.1926930064061	25.69611915562685	21.326583730837466
16	21.235219751617464	28.94166516163988	26.22836745317596	23.5947476335667
17	21.70478810993424	27.60148307110455	26.472181793071208	24.221547025890004
18	22.633301108053843	26.253333191683755	29.106863984531866	22.00650171573054
19	24.712362821234688	26.187997322822937	28.69731963581893	20.40232022012345
20	24.465361365785252	25.39918622316183	29.102083311200587	21.03336909985233
21	22.86914765906363	27.016647366911368	28.894920800178483	21.219284173846532
22	22.174356468251016	27.178127888323473	28.631983766958115	22.0155318764674
23	22.52015850588023	27.08145204984649	29.228505561516645	21.169883882756643
24	22.467039913310458	27.188751606837425	27.78208628584177	22.56212219401035
25	22.822403297602225	27.267898309766387	29.158920205250244	20.750778187381147
26	22.54299950068523	27.370948379351738	28.651106460283227	21.4349456596798
27	22.913236090896536	27.05754868319009	28.66438610842567	21.3648291174877
28	21.914606550584836	27.04533140689904	29.446822976978403	21.59323906553772
29	23.115617928587366	26.97840198026113	27.710376185872583	22.195603905278926
30	22.94723199014119	26.396753391622134	29.378831178489094	21.277183439747578
31	23.168736521157136	26.476962466402494	28.358954201149487	21.99534681129089
32	20.906946849536276	26.704310042601108	29.204602194860247	23.18414091300237
33	21.356330142676537	26.915190855103106	29.501003941399567	22.22747506082079
34	21.54171403074504	26.872164795121588	29.24550351113897	22.340617662994404
35	22.19188560379904	27.497901815593494	28.644732229174856	21.66548035143261
36	22.720415599868264	27.33642129418139	28.1231076501397	21.820055455810643
37	23.00300651233945	26.87747665437857	27.90744616430643	22.212070668975556
38	22.33371224596033	26.993275186180664	28.086455821266558	22.586556746592443
39	21.98525427870263	26.931126432874038	28.70634979655579	22.377269491867544
40	21.944352962423906	27.941442063551087	28.75309415801719	21.36111081600782
41	22.54087475698244	26.24961489020387	28.52627776774427	22.683232585069426
42	21.31436645454642	27.768275451773626	28.671822711385442	22.24553538229451
43	22.28749907042463	26.696873439641344	28.285119357477505	22.730508132456524
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14.0
1	14.5
2	15.0
3	64.5
4	114.0
5	114.0
6	182.0
7	250.0
8	258.5
9	267.0
10	364.5
11	462.0
12	462.0
13	746.5
14	1031.0
15	1827.5
16	2624.0
17	2728.5
18	2833.0
19	2833.0
20	2427.5
21	2022.0
22	1931.5
23	1841.0
24	2276.0
25	2711.0
26	2711.0
27	3193.0
28	3675.0
29	4296.0
30	4917.0
31	5807.0
32	6697.0
33	6697.0
34	7908.5
35	9120.0
36	9801.0
37	10482.0
38	11143.0
39	11804.0
40	11804.0
41	12701.5
42	13599.0
43	14239.5
44	14880.0
45	14953.5
46	15027.0
47	15027.0
48	15454.0
49	15881.0
50	15573.5
51	15266.0
52	14986.5
53	14707.0
54	14707.0
55	13460.0
56	12213.0
57	10464.5
58	8716.0
59	7733.0
60	6750.0
61	6750.0
62	5553.0
63	4356.0
64	3339.5
65	2323.0
66	1957.0
67	1591.0
68	1591.0
69	1265.0
70	939.0
71	709.0
72	479.0
73	390.0
74	301.0
75	301.0
76	223.5
77	146.0
78	112.0
79	78.0
80	66.5
81	55.0
82	55.0
83	45.0
84	35.0
85	27.0
86	19.0
87	16.5
88	14.0
89	14.0
90	9.0
91	4.0
92	2.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	188258.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.56828395074844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.45301321692736	45.97254831135994
2	5.395901539953923	5.673065686451572
3	1.877450385998949	2.9608303498390507
4	1.085243118709834	2.281974736797374
5	0.7497675922557697	1.9706997843385143
6	0.48906673133664763	1.5425639282261576
7	0.3758942645810598	1.3832081505168439
8	0.28293116688896974	1.1898564735628765
9	0.23038680732387534	1.0899935195317063
>10	1.775393072228285	18.550074897215524
>50	0.18087385311830564	6.66957048306048
>100	0.10003637686431431	8.440013173410957
>500	0.0020209369063497835	0.6475156434255118
>1k	0.0020209369063497835	1.6280848622634896
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1630	0.8658330588872717	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1435	0.7622518033762177	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	691	0.3670494746571195	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	528	0.2804661687683923	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	409	0.21725504361036452	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	361	0.19175811917687427	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	326	0.17316661177745435	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	303	0.16094933548640697	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	295	0.15669984808082527	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	246	0.13067173772163734	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	243	0.12907817994454418	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	235	0.12482869253896249	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	227	0.12057920513338079	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	223	0.11845446143058994	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	220	0.11686090365349679	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	219	0.11632971772779908	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	218	0.11579853180210137	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	215	0.11420497402500823	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	215	0.11420497402500823	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	214	0.11367378809931052	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	211	0.1120802303222174	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	211	0.1120802303222174	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	210	0.11154904439651966	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	207	0.10995548661942654	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	206	0.10942430069372881	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	205	0.10889311476803111	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	201	0.10676837106524026	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	199	0.10570599921384484	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	195	0.10358125551105399	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	194	0.10305006958535627	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	193	0.10251888365965857	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	189	0.10039413995686769	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0010623718513954254	0.0	0.0
2	0.0	0.0	0.0010623718513954254	0.0	0.0
3	0.0	0.0	0.001593557777093138	0.0	0.0
4	0.0	0.0	0.001593557777093138	0.0	0.0
5	0.0	0.0	0.001593557777093138	0.0	0.0
6	0.0	0.0	0.0047806733312794145	0.0	0.0
7	0.0	0.0	0.010623718513954255	0.0	0.0
8	0.0	0.0	0.013279648142442818	0.0	0.0
9	0.0	0.0	0.01752913554802452	0.0	0.0
10	0.0026559296284885637	0.0	0.018591507399419945	0.0	0.0
11	0.0026559296284885637	0.0	0.03399589924465361	0.0	0.0
12	0.0026559296284885637	0.0	0.03452708517035133	0.0	0.0
13	0.0026559296284885637	0.0	0.036120642947444465	0.0	0.0
14	0.0026559296284885637	0.0	0.03983894442732845	5.311859256977127E-4	0.0
15	0.0026559296284885637	0.0	0.04249487405581702	5.311859256977127E-4	0.0
16	0.0026559296284885637	0.0	0.04727554738709643	5.311859256977127E-4	0.0
17	0.0026559296284885637	0.0	0.04886910516418957	0.0010623718513954254	0.0
18	0.0026559296284885637	0.0	0.049931477015584994	0.0010623718513954254	0.0
19	0.0026559296284885637	0.0	0.049931477015584994	0.0010623718513954254	0.0
20	0.0026559296284885637	0.0	0.051525034792678136	0.0010623718513954254	0.0
21	0.0026559296284885637	0.0	0.051525034792678136	0.001593557777093138	0.0
22	0.0026559296284885637	0.0	0.05258740664407356	0.002124743702790851	0.0
23	0.0026559296284885637	0.0	0.05364977849546899	0.0026559296284885637	0.0
24	0.0026559296284885637	0.0	0.05524333627256212	0.0053118592569771274	0.0
25	0.0026559296284885637	0.0	0.05524333627256212	0.006374231108372552	0.0
26	0.0026559296284885637	0.0	0.05524333627256212	0.006374231108372552	0.0
27	0.0026559296284885637	0.0	0.05524333627256212	0.010623718513954255	0.0
28	0.0026559296284885637	0.0	0.05524333627256212	0.023372180730699358	0.0
29	0.0026559296284885637	0.0	0.05577452219825983	0.05630570812395755	0.0
30	0.0026559296284885637	0.0	0.05577452219825983	0.10570599921384483	0.0
31	0.0026559296284885637	0.0	0.05577452219825983	0.20397539546792168	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGTT	20	0.0018380296	37.0	4
TACTACG	20	0.0018380296	37.0	2
TACGTTG	20	0.0018380296	37.0	5
CCGACGA	25	0.00548482	29.6	13
CGCCGTT	25	0.00548482	29.6	25
CCGCCGT	45	3.984893E-6	28.777779	24
GTCCGAC	35	8.841756E-4	26.428572	11
GTATCAA	675	0.0	26.31111	1
CCTTAGG	40	0.0019252269	23.125	1
TCTACAG	40	0.0019252269	23.125	5
TACTGTG	40	0.0019252269	23.125	7
ACATCGA	40	0.0019252269	23.125	20
CCTGCAT	40	0.0019252269	23.125	36
CTGACAA	50	2.6904888E-4	22.2	32
GACAATT	60	3.705023E-5	21.583334	34
GGTATCA	200	0.0	21.275002	1
ACAGTCC	45	0.0038141117	20.555557	8
GTCTCGT	45	0.0038141117	20.555557	34
ACCTGCA	45	0.0038141117	20.555557	35
TCGAGGT	45	0.0038141117	20.555557	23
>>END_MODULE