FastQCFastQC Report
Fri 10 Feb 2017
ERR1632839.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632839.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254685
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31911.25292027406404No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25460.9996662543926812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12010.4715629110469796No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8670.3404205194652217No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7740.3039048236056305No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7220.28348744527553643No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7070.27759781691108626No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6140.24108212105149496No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5490.21556039813887742No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4720.18532697253469974No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG4640.18218583740699296No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4420.1735477158057993No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4360.17119186446001924No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT4330.1700139387871292No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC4220.1656948779865324No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4140.1625537428588256No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4110.16137581718593555No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4070.15980524962208217No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3960.15548618882148538No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG3920.15391562125763197No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3780.1484186347841451No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG3730.14645542532932837No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG3710.14567014154740168No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3690.144884857765475No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3680.14449221587451166No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3650.14331429020162162No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC3650.14331429020162162No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG3640.14292164831065826No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG3630.14252900641969493No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3620.14213636452873157No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT3560.1397805131829515No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC3560.1397805131829515No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA3480.1366393780552447No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3470.13624673616428135No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT3430.13467616860042797No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3430.13467616860042797No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3360.13192767536368455No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT3350.1315350334727212No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG3280.12878654023597777No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT3260.12800125645405108No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT3250.12760861456308775No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3210.12603804699923435No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT3180.12486012132634432No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT3120.12250426998056423No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3100.12171898619863754No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3030.1189704929618941No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG2980.11700728350707737No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC2970.11661464161611404No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC2940.115436715943224No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA2880.11308086459744389No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2880.11308086459744389No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG2730.1071912362329937No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC2690.1056206686691403No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT2650.10405010110528692No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT2590.10169424975950685No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT2550.10012368219565344No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCC250.005488411529.617
TGTCCAG250.005488411529.610
GGTATCA5000.028.491
TATGGGT556.2296203E-726.909094
TCCACTC358.8503707E-426.4285721
ATGAAAG358.8503707E-426.4285719
ACAGTTG509.054853E-625.90000219
ACTATGG601.3305871E-624.6666662
CTCTCTA601.3305871E-624.66666626
GTTTCCA551.8951288E-523.54545413
GCTGGTA400.001927083423.12500232
CTGGTAT400.001927083423.12500233
ACTAGTG400.001927083423.1250028
AGTTGCA400.001927083423.12500221
AGATTTG400.001927083423.12500237
GTAAGAT400.001927083423.12500234
GCCGCTC1054.0017767E-1122.90476427
GTCAACC1054.0017767E-1122.90476415
GCTCATA1054.0017767E-1122.90476434
GTATCAA12900.022.3720931
Produced by FastQC (version 0.11.5)