##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632839.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	254685
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.718087048707226	33.0	33.0	33.0	27.0	33.0
2	31.24681076624065	33.0	33.0	33.0	27.0	33.0
3	31.655955395881186	33.0	33.0	33.0	27.0	33.0
4	31.780297229911458	33.0	33.0	33.0	27.0	33.0
5	31.89585173842197	33.0	33.0	33.0	33.0	33.0
6	34.710065374874844	37.0	37.0	37.0	27.0	37.0
7	34.85808744134912	37.0	37.0	37.0	27.0	37.0
8	35.07732296758741	37.0	37.0	37.0	33.0	37.0
9	35.14517148634588	37.0	37.0	37.0	33.0	37.0
10	35.17070106209631	37.0	37.0	37.0	33.0	37.0
11	35.20819443626441	37.0	37.0	37.0	33.0	37.0
12	35.11355988770442	37.0	37.0	37.0	33.0	37.0
13	35.17237764297073	37.0	37.0	37.0	33.0	37.0
14	35.11088599642696	37.0	37.0	37.0	33.0	37.0
15	35.17342207040069	37.0	37.0	37.0	33.0	37.0
16	35.17226770324126	37.0	37.0	37.0	33.0	37.0
17	35.16760311757661	37.0	37.0	37.0	33.0	37.0
18	35.13751104305318	37.0	37.0	37.0	33.0	37.0
19	35.168765337573866	37.0	37.0	37.0	33.0	37.0
20	35.04233072226476	37.0	37.0	37.0	27.0	37.0
21	35.12273985511514	37.0	37.0	37.0	33.0	37.0
22	35.14563087735831	37.0	37.0	37.0	33.0	37.0
23	35.12128708011858	37.0	37.0	37.0	33.0	37.0
24	35.125939886526496	37.0	37.0	37.0	33.0	37.0
25	35.11560947837525	37.0	37.0	37.0	33.0	37.0
26	35.02067652197813	37.0	37.0	37.0	27.0	37.0
27	34.98963818049748	37.0	37.0	37.0	27.0	37.0
28	34.97321789661739	37.0	37.0	37.0	27.0	37.0
29	35.02618921412726	37.0	37.0	37.0	27.0	37.0
30	34.95065276714373	37.0	37.0	37.0	27.0	37.0
31	34.920584251133754	37.0	37.0	37.0	27.0	37.0
32	34.9424504780415	37.0	37.0	37.0	27.0	37.0
33	34.9086597169052	37.0	37.0	37.0	27.0	37.0
34	34.835086479376486	37.0	37.0	37.0	27.0	37.0
35	34.826322712370185	37.0	37.0	37.0	27.0	37.0
36	34.77652001492039	37.0	37.0	37.0	27.0	37.0
37	34.761042071578615	37.0	37.0	37.0	27.0	37.0
38	34.5898109429295	37.0	37.0	37.0	27.0	37.0
39	34.64805151461609	37.0	37.0	37.0	27.0	37.0
40	34.64199697665744	37.0	37.0	37.0	27.0	37.0
41	34.633472721204626	37.0	37.0	37.0	27.0	37.0
42	34.42322869426939	37.0	37.0	37.0	27.0	37.0
43	34.2040756228282	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	7.0
16	10.0
17	14.0
18	10.0
19	8.0
20	23.0
21	53.0
22	136.0
23	388.0
24	834.0
25	1535.0
26	2424.0
27	3540.0
28	4815.0
29	6656.0
30	8718.0
31	10923.0
32	13782.0
33	17883.0
34	24719.0
35	44104.0
36	114103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.62133616035495	19.394546204134517	13.62035455562754	23.363763079882993
2	17.440760154700904	23.80941162612639	36.9801912166009	21.769637002571805
3	19.02860396175668	27.392661523057892	30.049276557315903	23.529457957869525
4	12.27594872096904	18.285332862163063	39.45108663643324	29.987631780434654
5	14.610204762746138	36.342148143785465	33.486856312700006	15.5607907807684
6	27.396195300076563	40.98160472740837	16.801539156212577	14.820660816302492
7	26.401240748375443	32.62893378094509	21.694642401397804	19.275183069281663
8	25.130651589218054	33.043170975911416	20.11700728350708	21.70917015136345
9	27.583485482066084	13.426782103382608	19.167991833048667	39.82174058150264
10	16.916190588373873	26.482124977913895	31.438443567544223	25.163240866168014
11	35.434360091878204	23.681803011563304	22.101812042326795	18.7820248542317
12	24.268802638553506	25.994071107446455	28.240375365647765	21.496750888352278
13	30.288002827021614	22.144217366550837	24.696389657812592	22.871390148614957
14	23.497653964701495	20.900327855978954	28.205430237352026	27.396587941967525
15	26.255963248719006	26.527671437265642	25.97718750613503	21.23917780788032
16	21.962031529143843	27.325519759703166	25.584545615171685	25.127903095981306
17	22.93774662818776	25.549600486875946	25.252763217307656	26.259889667628638
18	23.963719889274987	24.059917152561006	28.60788817558945	23.368474782574552
19	26.764434497516543	23.915424936686495	27.96434811630053	21.355792449496434
20	26.23515322849795	23.015882364489467	28.40332175039755	22.345642656615034
21	23.856921294932956	25.498557041050713	28.2945599466007	22.34996171741563
22	23.266780532815044	25.353672183285237	27.989869839213142	23.389677444686573
23	23.400278775742585	25.406286196674323	29.086518640673773	22.10691638690932
24	23.584427822604393	25.536643304474154	27.08325971297878	23.795669159942676
25	23.613875964426644	25.886487229322498	29.06688654612561	21.432750260125253
26	24.106248895694684	25.5425329328386	28.003612305396864	22.34760586606985
27	24.30924475332273	25.40746412234721	28.07193199442449	22.21135912990557
28	22.55727663584428	25.60496299350178	28.53328621630642	23.304474154347528
29	24.625321475548226	25.461256061409195	27.04478080766437	22.868641655378212
30	24.16593046312111	24.691677955121033	28.924357539705913	22.218034042051947
31	24.066592064707386	24.73408327934507	27.87561104894281	23.32371360700473
32	21.532873942320908	24.891140035730412	28.863890688497555	24.712095333451124
33	21.870545968549386	25.09295796768557	29.754009855311462	23.28248620845358
34	22.13950566385928	25.271610028073894	28.965192296366098	23.62369201170073
35	23.001747256414788	25.954021634568193	27.817892690971203	23.22633841804582
36	23.67787659265367	25.54331821662053	27.970237744664978	22.80856744606082
37	23.80705577478061	25.212321102538432	27.71619844121169	23.26442468146927
38	23.238510316665682	25.422777156094785	27.447238745901803	23.89147378133773
39	22.71747452735732	25.138111785146357	28.3620943518464	23.78231933564992
40	22.83801558788307	26.57282525472643	28.287885034454323	22.301274122936178
41	23.388499519013685	24.73369063745411	28.218387419753814	23.65942242377839
42	22.25023067711094	26.53905805210358	28.259222176414	22.951489094371478
43	23.345308911007713	25.25904548756307	27.661621218367788	23.734024383061428
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	23.5
2	41.0
3	163.5
4	286.0
5	286.0
6	404.5
7	523.0
8	532.0
9	541.0
10	708.0
11	875.0
12	875.0
13	1376.0
14	1877.0
15	3076.5
16	4276.0
17	4438.5
18	4601.0
19	4601.0
20	3472.5
21	2344.0
22	1692.5
23	1041.0
24	1061.5
25	1082.0
26	1082.0
27	1337.5
28	1593.0
29	2067.0
30	2541.0
31	3591.5
32	4642.0
33	4642.0
34	6512.5
35	8383.0
36	9167.5
37	9952.0
38	11829.0
39	13706.0
40	13706.0
41	15453.5
42	17201.0
43	19100.5
44	21000.0
45	21682.5
46	22365.0
47	22365.0
48	23437.0
49	24509.0
50	24560.0
51	24611.0
52	23978.5
53	23346.0
54	23346.0
55	21568.0
56	19790.0
57	16995.0
58	14200.0
59	12773.5
60	11347.0
61	11347.0
62	9376.5
63	7406.0
64	5514.5
65	3623.0
66	3197.0
67	2771.0
68	2771.0
69	2291.5
70	1812.0
71	1441.0
72	1070.0
73	872.0
74	674.0
75	674.0
76	457.5
77	241.0
78	173.5
79	106.0
80	87.0
81	68.0
82	68.0
83	64.0
84	60.0
85	72.5
86	85.0
87	69.5
88	54.0
89	54.0
90	44.5
91	35.0
92	18.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	254685.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.1864263698294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.61665894063971	24.33830025325402
2	10.008051333349599	6.442468146926596
3	4.052504452631322	3.9130690853407155
4	2.206797277185449	2.8411567230107777
5	1.4346012150193963	2.3087343188644796
6	0.9661600019518385	1.8658342658578246
7	0.7002220216166102	1.577635117890728
8	0.5611535364871789	1.4449221587451166
9	0.4269646473272013	1.2368219565345426
>10	3.279088491472906	21.105679564952784
>50	0.43672383926610875	9.889471307693817
>100	0.30009515212140436	18.649704536977048
>500	0.007319393954180594	1.6620531244478474
>1k	0.003659696977090297	2.7241494395037007
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3191	1.25292027406404	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2546	0.9996662543926812	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1201	0.4715629110469796	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	867	0.3404205194652217	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	774	0.3039048236056305	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	722	0.28348744527553643	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	707	0.27759781691108626	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	614	0.24108212105149496	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	549	0.21556039813887742	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	472	0.18532697253469974	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	464	0.18218583740699296	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	442	0.1735477158057993	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	436	0.17119186446001924	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	433	0.1700139387871292	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	422	0.1656948779865324	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	414	0.1625537428588256	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	411	0.16137581718593555	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	407	0.15980524962208217	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	396	0.15548618882148538	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	392	0.15391562125763197	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	378	0.1484186347841451	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	373	0.14645542532932837	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	371	0.14567014154740168	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	369	0.144884857765475	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	368	0.14449221587451166	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	365	0.14331429020162162	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	365	0.14331429020162162	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	364	0.14292164831065826	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	363	0.14252900641969493	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	362	0.14213636452873157	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	356	0.1397805131829515	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	356	0.1397805131829515	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	348	0.1366393780552447	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	347	0.13624673616428135	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	343	0.13467616860042797	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	343	0.13467616860042797	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	336	0.13192767536368455	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	335	0.1315350334727212	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	328	0.12878654023597777	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	326	0.12800125645405108	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	325	0.12760861456308775	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	321	0.12603804699923435	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT	318	0.12486012132634432	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	312	0.12250426998056423	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	310	0.12171898619863754	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	303	0.1189704929618941	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	298	0.11700728350707737	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	297	0.11661464161611404	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	294	0.115436715943224	No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA	288	0.11308086459744389	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	288	0.11308086459744389	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	273	0.1071912362329937	No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC	269	0.1056206686691403	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	265	0.10405010110528692	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	259	0.10169424975950685	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	255	0.10012368219565344	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	7.852837819266938E-4	3.926418909633469E-4	0.0
2	3.926418909633469E-4	0.0	7.852837819266938E-4	3.926418909633469E-4	0.0
3	3.926418909633469E-4	0.0	0.0015705675638533875	3.926418909633469E-4	0.0
4	3.926418909633469E-4	0.0	0.0019632094548167342	7.852837819266938E-4	0.0
5	3.926418909633469E-4	0.0	0.002748493236743428	7.852837819266938E-4	0.0
6	3.926418909633469E-4	0.0	0.00628227025541355	7.852837819266938E-4	0.0
7	3.926418909633469E-4	0.0	0.013349824292753793	7.852837819266938E-4	0.0
8	7.852837819266938E-4	0.0	0.014527749965643835	7.852837819266938E-4	0.0
9	0.0011779256728900406	0.0	0.019239452657203997	0.0011779256728900406	0.0
10	0.0019632094548167342	0.0	0.022380587784910772	0.0011779256728900406	0.0
11	0.002355851345780081	0.0	0.03651569585959126	0.0011779256728900406	0.0
12	0.002355851345780081	0.0	0.03926418909633469	0.0011779256728900406	0.0
13	0.002355851345780081	0.0	0.04162004044211477	0.0011779256728900406	0.0
14	0.002355851345780081	0.0	0.04397589178789485	0.0011779256728900406	0.0
15	0.002748493236743428	0.0	0.04868759447945501	0.0011779256728900406	0.0
16	0.002748493236743428	0.0	0.050650803934271744	0.0015705675638533875	0.0
17	0.002748493236743428	0.0	0.05143608771619844	0.0015705675638533875	0.0
18	0.002748493236743428	0.0	0.05379193906197852	0.0019632094548167342	0.0
19	0.002748493236743428	0.0	0.05654043229872195	0.0019632094548167342	0.0
20	0.002748493236743428	0.0	0.058503641753538685	0.0019632094548167342	0.0
21	0.002748493236743428	0.0	0.06125213499028211	0.0019632094548167342	0.0
22	0.002748493236743428	0.0	0.06400062822702554	0.002355851345780081	0.0
23	0.002748493236743428	0.0	0.06478591200895223	0.003141135127706775	0.0
24	0.002748493236743428	0.0	0.06478591200895223	0.004711702691560162	0.0
25	0.002748493236743428	0.0	0.06478591200895223	0.005889628364450204	0.0
26	0.002748493236743428	0.0	0.06478591200895223	0.00628227025541355	0.0
27	0.002748493236743428	0.0	0.06478591200895223	0.01020868916504702	0.0
28	0.002748493236743428	0.0	0.06478591200895223	0.03298191884092114	0.0
29	0.002748493236743428	0.0	0.06478591200895223	0.07852837819266938	0.0
30	0.002748493236743428	0.0	0.06478591200895223	0.1382099456190981	0.0
31	0.002748493236743428	0.0	0.06517855389991559	0.24775703319787187	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCC	25	0.0054884115	29.6	17
TGTCCAG	25	0.0054884115	29.6	10
GGTATCA	500	0.0	28.49	1
TATGGGT	55	6.2296203E-7	26.90909	4
TCCACTC	35	8.8503707E-4	26.42857	21
ATGAAAG	35	8.8503707E-4	26.42857	19
ACAGTTG	50	9.054853E-6	25.900002	19
ACTATGG	60	1.3305871E-6	24.666666	2
CTCTCTA	60	1.3305871E-6	24.666666	26
GTTTCCA	55	1.8951288E-5	23.545454	13
GCTGGTA	40	0.0019270834	23.125002	32
CTGGTAT	40	0.0019270834	23.125002	33
ACTAGTG	40	0.0019270834	23.125002	8
AGTTGCA	40	0.0019270834	23.125002	21
AGATTTG	40	0.0019270834	23.125002	37
GTAAGAT	40	0.0019270834	23.125002	34
GCCGCTC	105	4.0017767E-11	22.904764	27
GTCAACC	105	4.0017767E-11	22.904764	15
GCTCATA	105	4.0017767E-11	22.904764	34
GTATCAA	1290	0.0	22.372093	1
>>END_MODULE