FastQCFastQC Report
Fri 10 Feb 2017
ERR1632838.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632838.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230707
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT80523.4901411747367876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT65882.8555700520573715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31621.37056959693464No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19590.8491289817820873No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG12700.550481779919985No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9450.40961045828691806No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9390.40700975696446146No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9140.3961735014542255No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG8620.37363408999293474No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8470.3671323366867932No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7060.3060158556090626No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6540.28347644414777184No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6390.2769746908416303No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6310.2735070890783548No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC5850.25356837893952067No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5780.25053422739665465No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5610.2431655736496942No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5510.2388310714455998No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5380.23319621858027714No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5210.22582756483331673No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT4980.2158582097638997No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4710.20415505381284485No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4580.1985202009475222No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4550.19721985028629388No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4480.1941856987434278No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4470.1937522485230184No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4300.18638359477605795No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4270.18508324411482963No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4220.18291599301278244No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4120.17858149080868807No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4110.17814804058827863No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4090.17728114014745977No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3990.1729466379433654No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG3980.17251318772295596No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3930.17034593662090877No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3900.16904558595968044No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3880.1681786855188616No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3850.1668783348576333No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT3820.16557798419640496No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3650.15820933044944455No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT3620.15690897978821622No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3500.15170757714330296No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3480.1508406767024841No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3400.1473730749392086No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC3390.14693962471879915No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3370.1460727242779803No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3350.1452058238371614No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA3350.1452058238371614No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG3290.1426051225147048No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC3260.14130477185347648No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3240.14043787141265762No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC3220.13957097097183874No Hit
CTTCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTCGCCG3120.13523646876774437No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT3120.13523646876774437No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3060.13263576744528774No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3050.1322023172248783No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3040.13176886700446888No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3030.13133541678405944No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG2990.1296016159024217No Hit
GTTTCATACTAGTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2970.1287347154616028No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2970.1287347154616028No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2970.1287347154616028No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC2920.12656746435955563No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG2870.12440021325750845No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA2710.11746500973095744No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT2690.11659810929013857No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC2640.11443085818809139No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2630.11399740796768196No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA2580.11183015686563476No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2580.11183015686563476No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2570.11139670664522532No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2560.1109632564248159No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2560.1109632564248159No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG2560.1109632564248159No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2530.10966290576358759No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC2520.10922945554317814No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.10792910488194984No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG2490.10792910488194984No Hit
TATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCGG2460.10662875422072153No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG2460.10662875422072153No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC2450.10619530400031209No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG2440.10576185377990265No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA2400.10402805289826489No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2380.10316115245744603No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG2330.10099390135539883No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC2320.1005604511349894No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC200.001838888937.04
AGTCCAA250.005487353529.611
GGGCTAG250.005487353529.618
ACTAGTC502.7048736E-729.68
GTCCAAA250.005487353529.612
GCTCTCT3250.026.18461430
CCGCTCT3200.026.01562528
GCCGCTC3200.026.01562527
CGCTCTC3200.026.01562529
CTAGTCC509.050027E-625.9000029
GCCGGCA3400.025.5735315
CAGCTTC3400.025.5735320
GCAGCTT3400.025.02941119
GGCAGCT3450.024.66666618
CGGCAGC3450.024.66666617
AGTGCAA451.3187385E-424.66666611
CTAGTGC451.3187385E-424.6666669
TAGTGCA451.3187385E-424.66666610
CTCTCTT3450.024.66666631
CGCCGGC3550.024.49295814
Produced by FastQC (version 0.11.5)