FastQCFastQC Report
Fri 10 Feb 2017
ERR1632837.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632837.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142206
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2110.14837629917162426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1870.13149937414736368No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC1710.12024809079785664No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGTC251.2281931E-437.028
CGGTCAC200.001836517637.030
TTTTTCG303.583777E-430.83333225
GGTATCA801.8189894E-1230.0625021
GGGTAGG250.00548036129.61
TTGAACG250.00548036129.610
CAACTAT250.00548036129.61
GAGCTAT358.83107E-426.42857211
TCTATAC358.83107E-426.4285723
CTATAGC400.001922921323.1250024
ATTCCTA400.001922921323.12500211
TGAGCTA400.001922921323.12500210
TAACTAG400.001922921323.12500226
ACTATAG502.6860012E-422.23
TTCATAC502.6860012E-422.23
TGTACTG705.050242E-621.1428595
TACCAAC450.003809590620.5555557
ACACCTA450.003809590620.55555512
ATTAACT450.003809590620.55555524
ATTCATA450.003809590620.5555552
Produced by FastQC (version 0.11.5)