##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632837.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	142206
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.165400897289846	33.0	33.0	33.0	14.0	33.0
2	30.14560567064681	33.0	33.0	33.0	14.0	33.0
3	30.629488207248638	33.0	33.0	33.0	27.0	33.0
4	30.800824156505353	33.0	33.0	33.0	27.0	33.0
5	30.90344289270495	33.0	33.0	33.0	27.0	33.0
6	32.755284587148225	37.0	33.0	37.0	14.0	37.0
7	33.07766198331997	37.0	33.0	37.0	14.0	37.0
8	33.1860399701841	37.0	33.0	37.0	14.0	37.0
9	33.1672784551988	37.0	33.0	37.0	14.0	37.0
10	33.227409532649816	37.0	33.0	37.0	27.0	37.0
11	33.146104946345446	37.0	33.0	37.0	14.0	37.0
12	33.34945782878359	37.0	33.0	37.0	27.0	37.0
13	33.381692755579934	37.0	33.0	37.0	27.0	37.0
14	33.38149585812132	37.0	33.0	37.0	27.0	37.0
15	33.43532621689661	37.0	33.0	37.0	27.0	37.0
16	33.43730925558697	37.0	33.0	37.0	27.0	37.0
17	33.40129108476435	37.0	33.0	37.0	27.0	37.0
18	33.42561495295557	37.0	33.0	37.0	27.0	37.0
19	33.41157897697706	37.0	33.0	37.0	27.0	37.0
20	33.419110304769134	37.0	33.0	37.0	27.0	37.0
21	33.39960339226193	37.0	33.0	37.0	27.0	37.0
22	33.4181820738928	37.0	33.0	37.0	27.0	37.0
23	33.375300620226994	37.0	33.0	37.0	27.0	37.0
24	33.38749419855702	37.0	33.0	37.0	27.0	37.0
25	33.3398028212593	37.0	33.0	37.0	27.0	37.0
26	33.31156913210413	37.0	33.0	37.0	22.0	37.0
27	33.23233900116732	37.0	33.0	37.0	14.0	37.0
28	33.206257119952745	37.0	33.0	37.0	14.0	37.0
29	33.21073653713627	37.0	33.0	37.0	14.0	37.0
30	33.14527516419842	37.0	33.0	37.0	14.0	37.0
31	33.14050743287906	37.0	33.0	37.0	14.0	37.0
32	33.15150556235321	37.0	33.0	37.0	14.0	37.0
33	33.144600087197446	37.0	33.0	37.0	14.0	37.0
34	33.04812033247542	37.0	33.0	37.0	14.0	37.0
35	33.038155914659015	37.0	33.0	37.0	14.0	37.0
36	33.04283926135325	37.0	33.0	37.0	14.0	37.0
37	33.07423034189837	37.0	33.0	37.0	14.0	37.0
38	32.983200427548766	37.0	33.0	37.0	14.0	37.0
39	33.040131921297274	37.0	33.0	37.0	14.0	37.0
40	33.0303433047832	37.0	33.0	37.0	14.0	37.0
41	33.08219765692024	37.0	33.0	37.0	14.0	37.0
42	32.9698676567796	37.0	33.0	37.0	14.0	37.0
43	32.65926191581227	37.0	33.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	12.0
16	14.0
17	15.0
18	14.0
19	38.0
20	108.0
21	259.0
22	545.0
23	1047.0
24	1760.0
25	2572.0
26	3606.0
27	4705.0
28	6031.0
29	7215.0
30	8590.0
31	10030.0
32	12126.0
33	14442.0
34	18259.0
35	25048.0
36	25769.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.8682193437689	22.940663544435537	12.148573196630242	25.04254391516532
2	16.906459643053033	25.02355737451303	34.144832144916535	23.925150837517403
3	18.43241494732993	27.936233351616668	29.72659381460698	23.904757886446422
4	14.292645879920679	20.51108954615136	37.09407479290607	28.102189781021895
5	15.382613954404173	37.179162623236714	35.087830330647094	12.350393091712023
6	29.611971365483875	40.52923224055244	17.315725074891354	12.543071319072332
7	26.390588301478136	32.56402683431079	23.983516869892973	17.061867994318103
8	23.94624699379773	36.92530554266346	20.5785972462484	18.54985021729041
9	25.16771444242859	15.662489627723161	20.44147223042628	38.72832369942196
10	14.39179781443821	29.079645022010325	34.48940269749518	22.039154466056285
11	33.90081993727409	23.667074525688086	24.388563070475225	18.04354246656259
12	22.283166673698716	26.07555236769194	30.89110164128096	20.75017931732838
13	27.310380715300337	22.63406607316147	26.316751754496998	23.738801457041195
14	21.224842833635712	21.361264644248486	27.588849978200635	29.825042543915163
15	23.390011673206477	29.89465985964024	23.81896685090643	22.896361616246853
16	23.130528950958468	28.774453961154943	25.4384484480261	22.656568639860485
17	22.009619847263828	28.60498150570299	26.766099883267934	22.61929876376524
18	22.25996090179036	28.5255193170471	27.73019422527882	21.484325555883718
19	23.79998031025414	27.26185955585559	27.36452751641984	21.57363261747043
20	23.287343712642222	27.51220061038212	27.338508923674105	21.86194675330155
21	22.49834746775804	27.84411346919258	27.50657496870737	22.15096409434201
22	22.392866686356413	27.579708310479162	27.440473679029015	22.58695132413541
23	22.584841708507376	27.34413456534886	27.685892297090138	22.385131429053626
24	22.633362867952126	28.240721207262702	26.074145957273252	23.05176996751192
25	22.852762893267514	27.693627554392926	27.32585122990591	22.127758322433653
26	22.75361095874998	27.884196166125196	27.32233520385919	22.03985767126563
27	22.62914363669606	27.583927541735232	26.775944756198754	23.010984065369954
28	22.68610325865294	27.538219203127856	27.688001912718168	22.08767562550103
29	23.444158474325977	27.523451893731625	26.76539667805859	22.2669929538838
30	22.913238541271113	27.37718521018804	27.43414483214492	22.275431416395932
31	23.091852664444538	27.522748688522285	27.378591620606723	22.006807026426454
32	22.067985879639394	27.545954460430643	27.294910200694765	23.091149459235194
33	22.068689084848742	27.67464101374063	27.977722458967975	22.278947442442654
34	23.020125733091433	26.955965289790868	27.51712304684753	22.50678593027017
35	22.821821864056368	27.82512692854029	27.15145633798855	22.20159486941479
36	23.610818108940553	27.469305092612128	26.57412486111697	22.345751937330352
37	22.698760952421136	27.351166617442303	27.19857108701461	22.751501343121948
38	22.832369942196532	27.600804466759488	27.03683388886545	22.529991702178528
39	22.40622758533395	27.413751881073935	27.71753653151063	22.46248400208149
40	22.469516054174928	27.921466042220437	27.73722627737226	21.87179162623237
41	22.436465409335753	27.15778518487265	27.29420699548542	23.11154241030618
42	22.41115002179936	27.71472371067325	27.405313418561803	22.468812848965587
43	23.289453328270255	26.706327440473682	27.336399308046072	22.667819923209994
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	3.5
2	7.0
3	14.5
4	22.0
5	22.0
6	31.5
7	41.0
8	35.0
9	29.0
10	44.0
11	59.0
12	59.0
13	115.5
14	172.0
15	278.5
16	385.0
17	455.0
18	525.0
19	525.0
20	589.5
21	654.0
22	831.0
23	1008.0
24	1411.0
25	1814.0
26	1814.0
27	2429.5
28	3045.0
29	3722.5
30	4400.0
31	5289.5
32	6179.0
33	6179.0
34	7080.0
35	7981.0
36	8935.0
37	9889.0
38	10706.5
39	11524.0
40	11524.0
41	11994.5
42	12465.0
43	12549.0
44	12633.0
45	12300.0
46	11967.0
47	11967.0
48	11385.5
49	10804.0
50	10432.5
51	10061.0
52	9480.5
53	8900.0
54	8900.0
55	8322.5
56	7745.0
57	6630.0
58	5515.0
59	5103.0
60	4691.0
61	4691.0
62	4137.0
63	3583.0
64	2933.5
65	2284.0
66	1900.0
67	1516.0
68	1516.0
69	1216.5
70	917.0
71	751.5
72	586.0
73	452.5
74	319.0
75	319.0
76	268.0
77	217.0
78	161.5
79	106.0
80	86.5
81	67.0
82	67.0
83	54.0
84	41.0
85	36.5
86	32.0
87	23.5
88	15.0
89	15.0
90	11.0
91	7.0
92	4.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	142206.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.44099959669948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.56246977897831	66.50998306514056
2	5.046024448962009	7.4117015904230925
3	1.4930559032464854	3.289545539645248
4	0.7418669092754145	2.1793378953960167
5	0.45191291578640624	1.6594468133006375
6	0.31493905388299254	1.3877663357523462
7	0.22395664933526674	1.1513320135456644
8	0.1679674864861685	0.9868560085831461
9	0.1319744536428792	0.8723102217085196
>10	0.8078436253192847	10.895066556862469
>50	0.040992065146148275	2.0552157496061865
>100	0.016996709938646843	1.6014382100361177
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	211	0.14837629917162426	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	187	0.13149937414736368	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	171	0.12024809079785664	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	7.032052093441908E-4	0.0	0.0
4	0.0	0.0	7.032052093441908E-4	0.0	0.0
5	0.0	0.0	7.032052093441908E-4	0.0	0.0
6	0.0	0.0	7.032052093441908E-4	0.0	0.0
7	0.0	0.0	7.032052093441908E-4	0.0	0.0
8	0.0	0.0	7.032052093441908E-4	0.0	0.0
9	0.0	0.0	0.0014064104186883816	0.0	0.0
10	0.0	0.0	0.0014064104186883816	0.0	0.0
11	0.0	0.0	0.0021096156280325726	7.032052093441908E-4	0.0
12	0.0	0.0	0.0021096156280325726	7.032052093441908E-4	0.0
13	0.0	0.0	0.003516026046720954	7.032052093441908E-4	0.0
14	0.0	0.0	0.003516026046720954	7.032052093441908E-4	0.0
15	0.0	0.0	0.003516026046720954	7.032052093441908E-4	0.0
16	0.0	0.0	0.004219231256065145	7.032052093441908E-4	0.0
17	0.0	0.0	0.004219231256065145	7.032052093441908E-4	0.0
18	0.0	0.0	0.004219231256065145	7.032052093441908E-4	0.0
19	0.0	0.0	0.004219231256065145	7.032052093441908E-4	0.0
20	0.0	0.0	0.004219231256065145	7.032052093441908E-4	0.0
21	0.0	0.0	0.004219231256065145	7.032052093441908E-4	0.0
22	0.0	0.0	0.004219231256065145	7.032052093441908E-4	0.0
23	0.0	0.0	0.004219231256065145	7.032052093441908E-4	0.0
24	0.0	0.0	0.004922436465409336	7.032052093441908E-4	0.0
25	0.0	0.0	0.005625641674753526	7.032052093441908E-4	0.0
26	0.0	0.0	0.005625641674753526	7.032052093441908E-4	0.0
27	0.0	0.0	0.005625641674753526	7.032052093441908E-4	0.0
28	0.0	0.0	0.005625641674753526	0.0021096156280325726	0.0
29	0.0	0.0	0.005625641674753526	0.010548078140162863	0.0
30	0.0	0.0	0.005625641674753526	0.02672179795507925	0.0
31	0.0	0.0	0.005625641674753526	0.06961731572507489	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGGTC	25	1.2281931E-4	37.0	28
CGGTCAC	20	0.0018365176	37.0	30
TTTTTCG	30	3.583777E-4	30.833332	25
GGTATCA	80	1.8189894E-12	30.062502	1
GGGTAGG	25	0.005480361	29.6	1
TTGAACG	25	0.005480361	29.6	10
CAACTAT	25	0.005480361	29.6	1
GAGCTAT	35	8.83107E-4	26.428572	11
TCTATAC	35	8.83107E-4	26.428572	3
CTATAGC	40	0.0019229213	23.125002	4
ATTCCTA	40	0.0019229213	23.125002	11
TGAGCTA	40	0.0019229213	23.125002	10
TAACTAG	40	0.0019229213	23.125002	26
ACTATAG	50	2.6860012E-4	22.2	3
TTCATAC	50	2.6860012E-4	22.2	3
TGTACTG	70	5.050242E-6	21.142859	5
TACCAAC	45	0.0038095906	20.555555	7
ACACCTA	45	0.0038095906	20.555555	12
ATTAACT	45	0.0038095906	20.555555	24
ATTCATA	45	0.0038095906	20.555555	2
>>END_MODULE