FastQCFastQC Report
Fri 10 Feb 2017
ERR1632836.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632836.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299821
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7790.25982169361052093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5220.17410388198291646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3190.10639681676733784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1500.027.1333331
GTATCAA4300.023.662791
GCACCGC551.896584E-523.54545410
GGTTGAA400.001927876823.1250021
GTCAACC603.714472E-521.58333415
GGGTAAG450.003819313420.5555551
CGGGCAT450.003819313420.55555510
ATTACCC656.880403E-519.9230773
GCGTCAG1052.242814E-819.3809511
TTACTCA801.6101843E-518.54
ACGGGAA801.6101843E-518.515
CTGCGTC902.141438E-618.59
CAGTGGG701.2153864E-418.59
TATACAG701.2153864E-418.55
ACCGACC500.007023055618.4999988
TGCGTCA1002.8573595E-718.49999810
GCATAAG1054.7680987E-717.6190471
AGGTACT852.712261E-517.4117644
GGCACCG752.0608518E-417.2666669
TAGGTAC752.0608518E-417.2666663
Produced by FastQC (version 0.11.5)