##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632836.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	299821
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.708532757878867	33.0	33.0	33.0	27.0	33.0
2	31.215725382811744	33.0	33.0	33.0	27.0	33.0
3	31.662682066966624	33.0	33.0	33.0	27.0	33.0
4	31.794940981452267	33.0	33.0	33.0	27.0	33.0
5	31.9135917764266	33.0	33.0	33.0	33.0	33.0
6	34.827597132955994	37.0	37.0	37.0	27.0	37.0
7	34.977890141117534	37.0	37.0	37.0	33.0	37.0
8	35.1415911493858	37.0	37.0	37.0	33.0	37.0
9	35.22481080377959	37.0	37.0	37.0	33.0	37.0
10	35.262159755320674	37.0	37.0	37.0	33.0	37.0
11	35.27277942505695	37.0	37.0	37.0	33.0	37.0
12	35.23688133919906	37.0	37.0	37.0	33.0	37.0
13	35.270484722551124	37.0	37.0	37.0	33.0	37.0
14	35.2427815263107	37.0	37.0	37.0	33.0	37.0
15	35.251146517422065	37.0	37.0	37.0	33.0	37.0
16	35.248128049736344	37.0	37.0	37.0	33.0	37.0
17	35.211729665367	37.0	37.0	37.0	33.0	37.0
18	35.22789931325691	37.0	37.0	37.0	33.0	37.0
19	35.25160345672918	37.0	37.0	37.0	33.0	37.0
20	35.239356149169005	37.0	37.0	37.0	33.0	37.0
21	35.22134540275698	37.0	37.0	37.0	33.0	37.0
22	35.23201843766781	37.0	37.0	37.0	33.0	37.0
23	35.2264050883694	37.0	37.0	37.0	33.0	37.0
24	35.205719412582845	37.0	37.0	37.0	33.0	37.0
25	35.218093462432584	37.0	37.0	37.0	33.0	37.0
26	35.18522718555405	37.0	37.0	37.0	33.0	37.0
27	35.12836325674319	37.0	37.0	37.0	33.0	37.0
28	35.094659813688835	37.0	37.0	37.0	33.0	37.0
29	35.13833253841459	37.0	37.0	37.0	33.0	37.0
30	35.08764896388178	37.0	37.0	37.0	33.0	37.0
31	35.07790648420224	37.0	37.0	37.0	33.0	37.0
32	35.10373189336304	37.0	37.0	37.0	33.0	37.0
33	35.09269530820056	37.0	37.0	37.0	33.0	37.0
34	35.037152167459915	37.0	37.0	37.0	27.0	37.0
35	35.026245659910415	37.0	37.0	37.0	27.0	37.0
36	35.00954903092178	37.0	37.0	37.0	27.0	37.0
37	35.03485412963068	37.0	37.0	37.0	27.0	37.0
38	34.89290610064005	37.0	37.0	37.0	27.0	37.0
39	34.962747772837794	37.0	37.0	37.0	27.0	37.0
40	35.00139082986182	37.0	37.0	37.0	27.0	37.0
41	35.03245936742256	37.0	37.0	37.0	27.0	37.0
42	34.95621387427832	37.0	37.0	37.0	27.0	37.0
43	34.72775422668859	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	8.0
16	15.0
17	20.0
18	9.0
19	21.0
20	27.0
21	56.0
22	154.0
23	425.0
24	779.0
25	1515.0
26	2489.0
27	3761.0
28	5278.0
29	7267.0
30	9173.0
31	11853.0
32	15125.0
33	19383.0
34	27748.0
35	51008.0
36	143706.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.226335046577795	21.21332394995681	13.522068167339846	25.038272836125554
2	15.894150176271843	23.14414267179417	37.50371054729322	23.45799660464077
3	17.399381631039855	26.981765786919524	30.35144302767318	25.267409554367436
4	12.585842886255467	18.109138452610058	39.57894877276775	29.726069888366723
5	13.5544208044133	37.39397840711624	35.932106156673484	13.119494631796972
6	29.627344315441544	40.305382211386124	17.395712775289258	12.67156069788307
7	26.271008368326438	32.264584535439475	24.247467655701236	17.21693944053285
8	24.320177706031266	36.151570437027424	20.88913051454034	18.639121342400966
9	25.543240800344208	15.327478728974956	20.479219267496273	38.65006120318456
10	14.25517225277749	28.521351072806773	34.88414754136635	22.339329133049386
11	33.8508643490616	23.692136307997107	24.519630045927403	17.937369297013888
12	22.48541629839137	25.77371164794994	30.891765420033952	20.84910663362473
13	27.42302907401416	22.471407940070907	26.437441006467193	23.668121979447736
14	21.547523355602177	20.981185440646254	28.093429079350678	29.377862124400895
15	23.35126625553247	29.255789287608273	24.424239796411857	22.9687046604474
16	22.85430306749694	28.659099929624677	25.391150052864873	23.095446950013507
17	22.022139876793155	28.226842015736054	26.87703663185701	22.873981475613782
18	22.374350028850547	28.03739564606882	27.925995844187028	21.662258480893602
19	23.90693113557756	26.989437030761685	27.467055343021336	21.636576490639413
20	23.253207747289213	27.19322529109035	27.64816340416448	21.905403557455948
21	22.677864459127278	27.4500451936322	27.475727183886384	22.396363163354135
22	22.357006347120446	27.272939520580614	27.57111743340193	22.79893669889701
23	22.540115602309378	27.067817130888095	27.961016739988192	22.43105052681433
24	22.602152617728578	27.742553056657137	26.71493991414878	22.940354411465506
25	22.828621077242754	27.219907878367426	27.551439025285084	22.40003201910473
26	22.639841772257448	27.615143702409107	27.41302310378526	22.33199142154819
27	22.657185453987548	27.673178329736743	26.971092752008698	22.69854346426701
28	22.465070825592605	27.309961610427557	27.883970769225634	22.340996794754204
29	23.42531043522635	27.146197231014508	26.99343941885325	22.435052914905892
30	22.829288141924682	27.20089653493251	27.378669272666023	22.591146050476784
31	22.810943863171694	27.153201410174738	27.489402009865888	22.546452716787684
32	22.11419480289906	27.085827877300122	27.6945243995584	23.10545292024241
33	22.107857688420758	27.15153374846992	28.143458930495196	22.597149632614126
34	22.866643764112588	26.844016930101628	27.698193255309	22.591146050476784
35	22.452396596635992	27.485399621774327	27.57011683637904	22.492086945210644
36	23.253207747289213	27.414023700808148	26.899049766360594	22.43371878554204
37	22.678531523809205	26.80666130791372	27.360658526254	23.154148642023074
38	22.76391580309585	27.36766270541423	27.272939520580614	22.595481970909308
39	22.41237271572038	27.079157230480856	27.65850290673435	22.849967147064415
40	22.432384656178186	27.42002728294549	28.00037355622188	22.147214504654443
41	22.500091721393765	26.759299715496915	27.57078390106097	23.169824662048356
42	22.259948435900085	27.556108478058576	27.5777880802212	22.60615500582014
43	22.697876399585084	26.561181504964633	27.704196837446343	23.036745258003943
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	12.0
2	18.0
3	54.0
4	90.0
5	90.0
6	114.0
7	138.0
8	121.5
9	105.0
10	159.0
11	213.0
12	213.0
13	329.5
14	446.0
15	771.5
16	1097.0
17	1358.5
18	1620.0
19	1620.0
20	1722.5
21	1825.0
22	2241.5
23	2658.0
24	3549.0
25	4440.0
26	4440.0
27	5759.0
28	7078.0
29	8618.5
30	10159.0
31	11820.0
32	13481.0
33	13481.0
34	15312.0
35	17143.0
36	18619.0
37	20095.0
38	21360.0
39	22625.0
40	22625.0
41	23477.0
42	24329.0
43	24430.0
44	24531.0
45	23973.0
46	23415.0
47	23415.0
48	22488.5
49	21562.0
50	21191.5
51	20821.0
52	19626.0
53	18431.0
54	18431.0
55	17408.0
56	16385.0
57	14441.5
58	12498.0
59	11598.5
60	10699.0
61	10699.0
62	9417.0
63	8135.0
64	6822.0
65	5509.0
66	4701.0
67	3893.0
68	3893.0
69	3230.0
70	2567.0
71	2064.0
72	1561.0
73	1286.5
74	1012.0
75	1012.0
76	766.0
77	520.0
78	412.0
79	304.0
80	235.5
81	167.0
82	167.0
83	130.0
84	93.0
85	87.0
86	81.0
87	63.0
88	45.0
89	45.0
90	33.5
91	22.0
92	13.0
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	299821.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.06161105752534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.54394558178008	66.15286535709757
2	5.4388419104990335	7.94741104536497
3	1.4462098583006555	3.1698726652416394
4	0.6714664723481292	1.9623368896347044
5	0.38548987028154835	1.408225548456319
6	0.2659511016157639	1.1658489567942807
7	0.19109953587098813	0.9773427974155825
8	0.14902404363706886	0.8710349371544968
9	0.10716352749886128	0.7046585969106803
>10	0.7246467723622634	10.102750761154283
>50	0.053679970597945256	2.6491566144710275
>100	0.021563748872677826	2.452388939829092
>500	9.176063350075672E-4	0.4361068904753484
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	779	0.25982169361052093	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	522	0.17410388198291646	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	319	0.10639681676733784	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.335323409634415E-4	0.0	0.0	0.0	0.0
3	3.335323409634415E-4	0.0	0.0	0.0	0.0
4	3.335323409634415E-4	0.0	0.0	0.0	0.0
5	3.335323409634415E-4	0.0	3.335323409634415E-4	0.0	0.0
6	3.335323409634415E-4	0.0	0.0010005970228903246	0.0	0.0
7	3.335323409634415E-4	0.0	0.002001194045780649	0.0	0.0
8	3.335323409634415E-4	0.0	0.002334726386744091	0.0	0.0
9	3.335323409634415E-4	0.0	0.002334726386744091	3.335323409634415E-4	0.0
10	6.67064681926883E-4	0.0	0.003335323409634415	3.335323409634415E-4	0.0
11	6.67064681926883E-4	0.0	0.005336517455415064	3.335323409634415E-4	0.0
12	6.67064681926883E-4	0.0	0.007337711501195714	3.335323409634415E-4	0.0
13	6.67064681926883E-4	0.0	0.007337711501195714	3.335323409634415E-4	0.0
14	6.67064681926883E-4	0.0	0.007337711501195714	3.335323409634415E-4	0.0
15	6.67064681926883E-4	0.0	0.00867184086504948	3.335323409634415E-4	0.0
16	6.67064681926883E-4	0.0	0.009672437887939804	3.335323409634415E-4	0.0
17	6.67064681926883E-4	0.0	0.009672437887939804	3.335323409634415E-4	0.0
18	6.67064681926883E-4	0.0	0.009672437887939804	3.335323409634415E-4	0.0
19	6.67064681926883E-4	0.0	0.009672437887939804	3.335323409634415E-4	0.0
20	6.67064681926883E-4	0.0	0.010339502569866687	3.335323409634415E-4	0.0
21	6.67064681926883E-4	0.0	0.010673034910830128	6.67064681926883E-4	0.0
22	6.67064681926883E-4	0.0	0.010673034910830128	6.67064681926883E-4	0.0
23	6.67064681926883E-4	0.0	0.010673034910830128	0.0010005970228903246	0.0
24	6.67064681926883E-4	0.0	0.010673034910830128	0.001334129363853766	0.0
25	6.67064681926883E-4	0.0	0.010673034910830128	0.001334129363853766	0.0
26	6.67064681926883E-4	0.0	0.010673034910830128	0.001334129363853766	0.0
27	6.67064681926883E-4	0.0	0.010673034910830128	0.002334726386744091	0.0
28	6.67064681926883E-4	0.0	0.010673034910830128	0.00867184086504948	0.0
29	6.67064681926883E-4	0.0	0.010673034910830128	0.0216796021626237	0.0
30	6.67064681926883E-4	0.0	0.010673034910830128	0.0450268660300646	0.0
31	6.67064681926883E-4	0.0	0.010673034910830128	0.10539621974444752	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	150	0.0	27.133333	1
GTATCAA	430	0.0	23.66279	1
GCACCGC	55	1.896584E-5	23.545454	10
GGTTGAA	40	0.0019278768	23.125002	1
GTCAACC	60	3.714472E-5	21.583334	15
GGGTAAG	45	0.0038193134	20.555555	1
CGGGCAT	45	0.0038193134	20.555555	10
ATTACCC	65	6.880403E-5	19.923077	3
GCGTCAG	105	2.242814E-8	19.38095	11
TTACTCA	80	1.6101843E-5	18.5	4
ACGGGAA	80	1.6101843E-5	18.5	15
CTGCGTC	90	2.141438E-6	18.5	9
CAGTGGG	70	1.2153864E-4	18.5	9
TATACAG	70	1.2153864E-4	18.5	5
ACCGACC	50	0.0070230556	18.499998	8
TGCGTCA	100	2.8573595E-7	18.499998	10
GCATAAG	105	4.7680987E-7	17.619047	1
AGGTACT	85	2.712261E-5	17.411764	4
GGCACCG	75	2.0608518E-4	17.266666	9
TAGGTAC	75	2.0608518E-4	17.266666	3
>>END_MODULE