FastQCFastQC Report
Fri 10 Feb 2017
ERR1632835.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632835.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299362
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60822.0316539841396035No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT47921.6007375685624763No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23950.8000347405482326No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA19310.645038448433669No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG17130.5722169146384645No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13920.4649888763436909No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13190.44060368383428755No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT12720.4249036283830279No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11960.39951630467460797No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC10160.3393884327336135No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG9230.30832236556409964No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9230.30832236556409964No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC9170.3063181031660665No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9090.30364575330202226No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8890.2969648786419118No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA8260.2759201234625637No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7940.2652307240063869No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7570.25287110588518247No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC7480.24986471228813276No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC7400.24719236242408854No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7310.24418596882703883No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7190.24017744403097255No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA7130.2381731816329394No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC7040.23516678803588964No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA6940.2318263507058344No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT6940.2318263507058344No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6880.22982208830780126No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT6830.22815186964277365No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA6720.22447738857971286No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT6660.22247312618167972No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT6590.22013482005064103No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6550.21879864511861893No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6540.21846460138561338No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6440.21512416405555815No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6150.20543689579839794No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5730.19140705901216587No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5680.18973684034713825No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC5680.18973684034713825No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG5610.1873985342160996No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5560.18572831555107194No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5540.1850602280850609No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA5370.17938148462396697No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5290.17670913475992278No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5230.1747048723618896No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5200.17370274116287304No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4950.1653516478377349No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT4900.16368142917270728No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4880.16301334170669624No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT4780.15967290437664097No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4620.1543282046485526No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4580.15299202971653048No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA4550.1519898985175139No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4540.15165585478450838No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4540.15165585478450838No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4510.1506537235854918No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4490.14998563611948076No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT4420.1476473299884421No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4380.14631115505642No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4240.14163454279434265No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4230.1413004990613371No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4210.14063241159532608No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG4180.13963028039630948No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG4160.13896219293029843No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA4160.13896219293029843No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA4140.1382941054642874No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4140.1382941054642874No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4070.13595579933324872No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG4060.1356217556002432No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC4060.1356217556002432No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4040.13495366813423212No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG4030.1346196244012266No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC4000.13361749320221003No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA3880.12960896840614372No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC3780.12626853107608849No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG3770.12593448734308296No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3740.12493235614406638No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3740.12493235614406638No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3720.12426426867805535No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG3650.12192596254701667No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3630.1212578750810056No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC3630.1212578750810056No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.12058978761499456No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT3610.12058978761499456No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG3590.1199217001489835No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG3540.11825148148395588No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG3540.11825148148395588No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3480.11624721908592273No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3330.11123656309083986No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT3330.11123656309083986No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3320.11090251935783432No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA3290.10990038815881775No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3250.10856421322679566No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3230.10789612576078461No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC3210.10722803829477355No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA3190.1065599508287625No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC3180.10622590709575698No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3180.10622590709575698No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA3150.10522377589674041No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3010.10054716363466305No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGTT200.001839762837.024
GCAACTT250.005489931429.613
AAGACTC250.005489931429.631
GAATCAA250.005489931429.61
GTTAAGA250.005489931429.628
AGACCCG250.005489931429.65
ACTCGGT250.005489931429.634
CAACTTA250.005489931429.614
TATACCC250.005489931429.65
CATTTAC250.005489931429.614
GACTCGG250.005489931429.633
GGTATCA8700.027.2183911
TCTTTGT358.854014E-426.42857237
CTAGTGC509.061792E-625.9000029
TAGTGCA509.061792E-625.90000210
TTGCTCC701.9130493E-723.78571513
CCCACGC701.9130493E-723.78571519
ACTAGTG1103.6379788E-1223.5454548
GGTCGCT400.001927870823.12528
GCTCTCG400.001927870823.12518
Produced by FastQC (version 0.11.5)